ID   NUCS_BIFLS              Reviewed;         256 AA.
AC   B7GTZ5; E8MP86;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722};
GN   OrderedLocusNames=BLIJ_0316, Blon_0311;
OS   Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM
OS   1222 / NCTC 11817 / S12).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=391904;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12;
RX   PubMed=19033196; DOI=10.1073/pnas.0809584105;
RA   Sela D.A., Chapman J., Adeuya A., Kim J.H., Chen F., Whitehead T.R.,
RA   Lapidus A., Rokhsar D.S., Lebrilla C.B., German J.B., Price N.P.,
RA   Richardson P.M., Mills D.A.;
RT   "The genome sequence of Bifidobacterium longum subsp. infantis reveals
RT   adaptations for milk utilization within the infant microbiome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:18964-18969(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12;
RX   PubMed=21270894; DOI=10.1038/nature09646;
RA   Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T.,
RA   Clarke J.M., Topping D.L., Suzuki T., Taylor T.D., Itoh K., Kikuchi J.,
RA   Morita H., Hattori M., Ohno H.;
RT   "Bifidobacteria can protect from enteropathogenic infection through
RT   production of acetate.";
RL   Nature 469:543-547(2011).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP001095; ACJ51436.1; -; Genomic_DNA.
DR   EMBL; AP010889; BAJ67910.1; -; Genomic_DNA.
DR   RefSeq; WP_007051476.1; NZ_JDTT01000024.1.
DR   AlphaFoldDB; B7GTZ5; -.
DR   SMR; B7GTZ5; -.
DR   PRIDE; B7GTZ5; -.
DR   EnsemblBacteria; ACJ51436; ACJ51436; Blon_0311.
DR   GeneID; 66504483; -.
DR   KEGG; bln:Blon_0311; -.
DR   KEGG; blon:BLIJ_0316; -.
DR   PATRIC; fig|391904.8.peg.319; -.
DR   HOGENOM; CLU_069350_0_1_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000001360; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 2.
DR   Pfam; PF01939; NucS; 2.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..256
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000198196"
FT   REGION          62..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   256 AA;  27867 MW;  428B743E320C7AA0 CRC64;
     MRVIVADCSA EYSGRLNASL PLAKRVLLIK ADSSLLIFSE LGSYKPLNWM TAPCTIREID
     PAAKSAQHSR ESVAGGAVDG DSATHSPESV AAGEPEKVLR VSADKGSDIL EVTLQHIYSD
     QTYDLGEDPG LIKDGVEDHL QRYLAEQIER IGKGAKLVRR EYPTAIGPVD IMAVNAEGEH
     VAVEIKRHGG IDGVEQLTRY CELLNRDPLL APVHGIFAAQ TITPQAQVLA KDRGFTCLIL
     DYDDMKGTED DSLRLF