NUCS_COREF
ID NUCS_COREF Reviewed; 230 AA.
AC Q8FQ17;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2003, sequence version 2.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=CE1318;
OS Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189
OS / NBRC 100395).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196164;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395;
RX PubMed=12840036; DOI=10.1101/gr.1285603;
RA Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S.,
RA Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.;
RT "Comparative complete genome sequence analysis of the amino acid
RT replacements responsible for the thermostability of Corynebacterium
RT efficiens.";
RL Genome Res. 13:1572-1579(2003).
CC -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC18128.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000035; BAC18128.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_035109476.1; NZ_GG700686.1.
DR AlphaFoldDB; Q8FQ17; -.
DR SMR; Q8FQ17; -.
DR STRING; 196164.23493157; -.
DR EnsemblBacteria; BAC18128; BAC18128; BAC18128.
DR KEGG; cef:CE1318; -.
DR eggNOG; COG1637; Bacteria.
DR HOGENOM; CLU_069350_0_0_11; -.
DR OrthoDB; 1002946at2; -.
DR Proteomes; UP000001409; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_00722; NucS; 1.
DR InterPro; IPR002793; Endonuclease_NucS.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR38814; PTHR38814; 1.
DR Pfam; PF01939; NucS; 1.
DR SUPFAM; SSF52980; SSF52980; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome.
FT CHAIN 1..230
FT /note="Endonuclease NucS"
FT /id="PRO_0000155681"
SQ SEQUENCE 230 AA; 25788 MW; C3BA2EBEB877E74D CRC64;
MRLVIARCSV DYIGRLEAHL PMADRLLMVK ADGSVSIHAD DRAYKPLNWM TPPCTLTETP
ITDEDGEEVG VHLWVVENKK GEQLRITVEK IHSELSHDLG VDPGLVKDGV EDHLQELLAE
HITTLGEGYT LVRREYPTAI GPVDILCRDA DGQTVAVEIK RRGNIDGVEQ LSRYLELLNR
DELLKPVQGV FAAQEIKPQA RTLAEDRGIR CVTLDYQTLR GIESNELTLF