ID   NUCS_CORGB              Reviewed;         230 AA.
AC   A4QDH7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=cgR_1294;
OS   Corynebacterium glutamicum (strain R).
OC   Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC   Corynebacterium.
OX   NCBI_TaxID=340322;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R;
RX   PubMed=17379713; DOI=10.1099/mic.0.2006/003657-0;
RA   Yukawa H., Omumasaba C.A., Nonaka H., Kos P., Okai N., Suzuki N., Suda M.,
RA   Tsuge Y., Watanabe J., Ikeda Y., Vertes A.A., Inui M.;
RT   "Comparative analysis of the Corynebacterium glutamicum group and complete
RT   genome sequence of strain R.";
RL   Microbiology 153:1042-1058(2007).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AP009044; BAF54274.1; -; Genomic_DNA.
DR   RefSeq; WP_003854828.1; NC_009342.1.
DR   AlphaFoldDB; A4QDH7; -.
DR   SMR; A4QDH7; -.
DR   EnsemblBacteria; BAF54274; BAF54274; cgR_1294.
DR   GeneID; 58309926; -.
DR   KEGG; cgt:cgR_1294; -.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   PhylomeDB; A4QDH7; -.
DR   Proteomes; UP000006698; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..230
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045828"
SQ   SEQUENCE   230 AA;  25616 MW;  A261E8B3944C35F6 CRC64;
     MRLVIARCSV DYVGRLEAHL PSADRLLMVK ADGSVSIHAD DRAYKPLNWM TPPCSLVETP
     ITDEDGEATG ESLWVVENKK GEQLRITVEK IHSEQNFDLG EDPGLVKDGV EDHLQELLAE
     HITTLGDGYT LIRREYPTAI GPVDILCRNS DGETVAVEIK RRGGIDGVEQ LTRYLELLNR
     DELLKPVHGV FAAQEIKPQA KTLAEDRGIK CVTLDYQALR GIESNELTLF