ID   NUCS_METKA              Reviewed;         260 AA.
AC   Q8TY00;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=MK0507;
OS   Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938).
OC   Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae;
OC   Methanopyrus.
OX   NCBI_TaxID=190192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
RX   PubMed=11930014; DOI=10.1073/pnas.032671499;
RA   Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N.,
RA   Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A.,
RA   Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G.,
RA   Koonin E.V., Kozyavkin S.A.;
RT   "The complete genome of hyperthermophile Methanopyrus kandleri AV19 and
RT   monophyly of archaeal methanogens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AE009439; AAM01722.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8TY00; -.
DR   SMR; Q8TY00; -.
DR   STRING; 190192.MK0507; -.
DR   EnsemblBacteria; AAM01722; AAM01722; MK0507.
DR   KEGG; mka:MK0507; -.
DR   PATRIC; fig|190192.8.peg.538; -.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000001826; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..260
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155692"
SQ   SEQUENCE   260 AA;  29041 MW;  7E099024A5000C57 CRC64;
     MTKVLVEPDP EEVKQLSEAL GRQPVILAGI CEAEYRGRAE SVAGPALRIA MCKPDGTFIL
     HNAMEKREPT NWNPAPSRQS IEVRDGCVVL RSRRLDVPEE VVVYFHKVLL ACSLPKEGAK
     SEDSVFSLFR SEEDMKRVIR EDPSVIEPGF RPVGEEVECG AGVADVVGYD EEGRFVVLEL
     KRTRAGVSAA SQLRRYVEAF REERGEEVRG ILVAPSVTDR CRRLLEKYGL EWKKLEPVPL
     RDDGGKKQCT LTEFLAGEGD