NUCS_METTH
ID NUCS_METTH Reviewed; 253 AA.
AC O27844;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=MTH_1816;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR EMBL; AE000666; AAB86282.1; -; Genomic_DNA.
DR PIR; G69109; G69109.
DR AlphaFoldDB; O27844; -.
DR SMR; O27844; -.
DR STRING; 187420.MTH_1816; -.
DR EnsemblBacteria; AAB86282; AAB86282; MTH_1816.
DR KEGG; mth:MTH_1816; -.
DR PATRIC; fig|187420.15.peg.1770; -.
DR HOGENOM; CLU_069350_1_0_2; -.
DR OMA; RCSVDYA; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_00722; NucS; 1.
DR InterPro; IPR002793; Endonuclease_NucS.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR38814; PTHR38814; 1.
DR Pfam; PF01939; NucS; 1.
PE 3: Inferred from homology;
KW Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome.
FT CHAIN 1..253
FT /note="Endonuclease NucS"
FT /id="PRO_0000155693"
SQ SEQUENCE 253 AA; 28975 MW; 83BA42407CCF0660 CRC64;
MNCMKCKVSE NPSIKEAYRL IEDGIRKRAL VVILACCSAS YEGRARSRLD AGERLIVIKP
DGTFMVHQDR KVDPVNWQPP KSRSRAYIKR GSLYLESIRR DPEERLEVEI HEAHLVSYYL
ARDVHDLMVA GHENDMGDMI IMHPHLIEKG FRPVAREYAV TSGFIDILGK DENGSLMIIE
LKSRKAGVSA VKQLKRYVDE FREDRVGVRG VLVAPSITHD AMEMLEEEGL EFREIEPPRE
LRSNRGVTLD NFL