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NUCS_MYCA9
ID   NUCS_MYCA9              Reviewed;         232 AA.
AC   B1MLW9;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=MAB_1460;
OS   Mycobacteroides abscessus (strain ATCC 19977 / DSM 44196 / CIP 104536 / JCM
OS   13569 / NCTC 13031 / TMC 1543) (Mycobacterium abscessus).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacteroides; Mycobacteroides abscessus.
OX   NCBI_TaxID=561007;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19977 / DSM 44196 / CIP 104536 / JCM 13569 / NCTC 13031 / TMC
RC   1543;
RX   PubMed=19543527; DOI=10.1371/journal.pone.0005660;
RA   Ripoll F., Pasek S., Schenowitz C., Dossat C., Barbe V., Rottman M.,
RA   Macheras E., Heym B., Herrmann J.L., Daffe M., Brosch R., Risler J.L.,
RA   Gaillard J.L.;
RT   "Non mycobacterial virulence genes in the genome of the emerging pathogen
RT   Mycobacterium abscessus.";
RL   PLoS ONE 4:E5660-E5660(2009).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CU458896; CAM61546.1; -; Genomic_DNA.
DR   RefSeq; WP_005059861.1; NZ_MLCG01000002.1.
DR   AlphaFoldDB; B1MLW9; -.
DR   SMR; B1MLW9; -.
DR   EnsemblBacteria; CAM61546; CAM61546; MAB_1460.
DR   GeneID; 66971844; -.
DR   KEGG; mab:MAB_1460; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000007137; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..232
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000198201"
SQ   SEQUENCE   232 AA;  25523 MW;  AFC2C4D93BF0CFC2 CRC64;
     MRLVVAQCTV NYVGRLTAHL PSAKRLLLIK ADGSVSVHAD DRAYKPLNWM SPPCWLTETE
     GEGGDDTSES GAPTVWVVEN KAGEQLRITI ESIEHDSAHE LGVDPGLVKD GVEAHLQELL
     AEHVALLGDG YTLVRREYMT PIGPVDLLCR DADGATVAVE IKRRGEIDGV EQLTRYLELL
     NRDTTLAPVA GVFAAQQIKP QARTLAEDRG IRCLTLDYDA MRGMDSDEFR LF
 
 
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