ID   NUCS_MYCLE              Reviewed;         220 AA.
AC   P53524; Q9CC77;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=ML1155;
GN   ORFNames=B1549_C3_223;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Smith D.R., Robison K.;
RL   Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA50894.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U00014; AAA50894.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AL583921; CAC31536.1; -; Genomic_DNA.
DR   PIR; E87053; E87053.
DR   PIR; S72806; S72806.
DR   RefSeq; NP_301846.1; NC_002677.1.
DR   AlphaFoldDB; P53524; -.
DR   SMR; P53524; -.
DR   STRING; 272631.ML1155; -.
DR   EnsemblBacteria; CAC31536; CAC31536; CAC31536.
DR   KEGG; mle:ML1155; -.
DR   PATRIC; fig|272631.5.peg.2086; -.
DR   Leproma; ML1155; -.
DR   eggNOG; COG1637; Bacteria.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..220
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155684"
SQ   SEQUENCE   220 AA;  24404 MW;  F0884930C4E0E565 CRC64;
     MIAQCTVDYL GRLTAHLPSA RRLLLFKADG SVSVHADDRA YKPLNWMSPP CWLTEDVAGA
     SPAWVVENKA GEQLRITVEE VEHDSCHDLG VDPGLVKDGV EAHLQTLLAE QVQLLGEGYT
     LVRCEYMTAI GPVDLLCRDE QGGSVAVEIK RRGEIDGVEQ LTRYLALLNR NSVLAPVRGV
     FAAQHIKPQA RTLATDRGIR CVTLDYDKMR GMDSDEYRLF