ID   NUCS_MYCS2              Reviewed;         224 AA.
AC   A0R1Z0; I7GED9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722};
GN   OrderedLocusNames=MSMEG_4923, MSMEI_4796;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AFP41244.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000480; ABK73844.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP41244.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_011730195.1; NZ_SIJM01000024.1.
DR   RefSeq; YP_889178.1; NC_008596.1.
DR   AlphaFoldDB; A0R1Z0; -.
DR   SMR; A0R1Z0; -.
DR   STRING; 246196.MSMEI_4796; -.
DR   EnsemblBacteria; ABK73844; ABK73844; MSMEG_4923.
DR   EnsemblBacteria; AFP41244; AFP41244; MSMEI_4796.
DR   GeneID; 66736227; -.
DR   KEGG; msg:MSMEI_4796; -.
DR   KEGG; msm:MSMEG_4923; -.
DR   PATRIC; fig|246196.19.peg.4804; -.
DR   eggNOG; COG1637; Bacteria.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 1002946at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..224
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045832"
SQ   SEQUENCE   224 AA;  25099 MW;  0AD91A3A6AB878EB CRC64;
     MRLVIAQCTV DYVGRLTAHL PSARRLLLFK ADGSVSVHAD DRAYKPLNWM SPPCWVTEQD
     TETGVALWVV ENKTGEQLRI TVEDIEHDSH HELGVDPGLV KDGVEAHLQA LLAEHVELLG
     AGYTLVRREY PTPIGPVDLL CRDELGRSVA VEIKRRGEID GVEQLTRYLE LLNRDSLLAP
     VAGVFAAQQI KPQARTLATD RGIRCVTLDY DQMRGMDSDE YRLF