ID   NUCS_MYCSJ              Reviewed;         223 AA.
AC   A3Q3A2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=Mjls_3853;
OS   Mycobacterium sp. (strain JLS).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; unclassified Mycobacterium.
OX   NCBI_TaxID=164757;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JLS;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Miller C.D., Anderson A.J.,
RA   Sims R.C., Richardson P.;
RT   "Complete sequence of Mycobacterium sp. JLS.";
RL   Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP000580; ABN99629.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3Q3A2; -.
DR   SMR; A3Q3A2; -.
DR   STRING; 164757.Mjls_3853; -.
DR   KEGG; mjl:Mjls_3853; -.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   BioCyc; MSP164757:G1G8C-3890-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..223
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045833"
SQ   SEQUENCE   223 AA;  25027 MW;  F3124CBAA4F7977A CRC64;
     MRLVIAQCTV DYVGRLTAHL PSARRLLLIK SDGSVSVHAD DRAYKPLNWM SPPCRLSEES
     GDPHPVWVVE NKTGEQLRIT VEEIEHDSSH DLGVDPGLVK DGVEAHLQKL LAEHVELLGA
     GYTLVRREYM TAIGPVDLLC RDETGRSVAV EIKRRGEIDG VEQLTRYLEL LNRDTLLAPV
     SGVFAAQQIK PQARTLANDR GIRCLILDYD QMRGMDSDEY RLF