ID   NUCS_PYRAE              Reviewed;         250 AA.
AC   Q8ZWG7;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=PAE1797;
OS   Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP
OS   104966 / NBRC 100827 / IM2).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=178306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2;
RX   PubMed=11792869; DOI=10.1073/pnas.241636498;
RA   Fitz-Gibbon S.T., Ladner H., Kim U.-J., Stetter K.O., Simon M.I.,
RA   Miller J.H.;
RT   "Genome sequence of the hyperthermophilic crenarchaeon Pyrobaculum
RT   aerophilum.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:984-989(2002).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AE009441; AAL63735.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8ZWG7; -.
DR   SMR; Q8ZWG7; -.
DR   STRING; 178306.PAE1797; -.
DR   DNASU; 1465987; -.
DR   EnsemblBacteria; AAL63735; AAL63735; PAE1797.
DR   KEGG; pai:PAE1797; -.
DR   PATRIC; fig|178306.9.peg.1328; -.
DR   eggNOG; arCOG01304; Archaea.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   InParanoid; Q8ZWG7; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000002439; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..250
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155695"
SQ   SEQUENCE   250 AA;  27450 MW;  53149557E391A526 CRC64;
     MQQAVGMYVE APTPDEAARL INSGRKLGIV IAVGVCEVFY TGRAAASLGP TRRLVILKRD
     GTLLVHEAEK AQPKIWNPPG SSTAAYVEGG KLVVKSIRSR PFETVRVIFD KVDFIAAFDV
     GATELTLVGS ERDVVGALVK NPSIIEEGLE VVGVEVPTDV GHLDILARDK NGRYVIIEVK
     RDLATHEAVF QLARYVELYR RKGYDVRGIL VAGDITASAY DYLKRYGLNF VKINPRELMV
     LTNKNPLNAK