ID   NUCS_PYRFU              Reviewed;         267 AA.
AC   Q8U4R1;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=PF0012;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AE009950; AAL80136.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8U4R1; -.
DR   SMR; Q8U4R1; -.
DR   STRING; 186497.PF0012; -.
DR   EnsemblBacteria; AAL80136; AAL80136; PF0012.
DR   KEGG; pfu:PF0012; -.
DR   PATRIC; fig|186497.12.peg.13; -.
DR   eggNOG; arCOG01304; Archaea.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   OMA; RCSVDYA; -.
DR   PhylomeDB; Q8U4R1; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..267
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155696"
SQ   SEQUENCE   267 AA;  30211 MW;  3941EF555CDC0002 CRC64;
     MEMTKAIVKE NPRIEEIKEL LEVAESREGL LTIFARCTVY YEGRAKSELG EGDRIIIIKP
     DGSFLIHQKK KREPVNWQPP GSKVKMEGNS LISIRRNPKE TLKVDIIEAY AAVLFMAEDY
     EELTLTGSEA EMAELIFQNP NVIEEGFKPM FREKPIKHGI VDVLGVDREG NIVVLELKRR
     RADLHAVSQL KRYVDALKEE HGNKVRGILV APSLTEGAKK LLEKLGLEFR KLEPPKKGKK
     KSSKQKTLDF LNDTVRITGA SPPEAIQ