ID   NUCS_PYRHO              Reviewed;         253 AA.
AC   O57868;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=PH0128;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; BA000001; BAA29197.1; -; Genomic_DNA.
DR   PIR; F71233; F71233.
DR   RefSeq; WP_010884242.1; NC_000961.1.
DR   AlphaFoldDB; O57868; -.
DR   SMR; O57868; -.
DR   STRING; 70601.3256514; -.
DR   EnsemblBacteria; BAA29197; BAA29197; BAA29197.
DR   GeneID; 1444024; -.
DR   KEGG; pho:PH0128; -.
DR   eggNOG; arCOG01304; Archaea.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 96363at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..253
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155697"
SQ   SEQUENCE   253 AA;  29058 MW;  FD601BFED45D3DCF CRC64;
     MKKVITRENP TVEEVKELLD IAEKHGGVVT IFARCRVYYE GRAKSELGEG DRIVIIKPDG
     SFLIHQNKKR EPVNWQPPGS KVSMRENSII SIRRKPHERL EVELMEVYAV TVFLAEDYEE
     LALTGSEAEM AKLIFENPNV IEEGFKPMFR EKQIKHGIVD IMGLDKDGNI VVLELKRRKA
     DLHAVSQLKR YVDSLKEEYG EKVRGILVAP SLTEGAKKLL EKEGLEFRKL EPPKNNDNKR
     EVKQKTLDFF TPC