ID   NUCS_RHOE4              Reviewed;         224 AA.
AC   C1A1X1;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=RER_38980;
OS   Rhodococcus erythropolis (strain PR4 / NBRC 100887).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC   Rhodococcus erythropolis group.
OX   NCBI_TaxID=234621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PR4 / NBRC 100887;
RA   Takarada H., Sekine M., Hosoyama A., Yamada R., Fujisawa T., Omata S.,
RA   Shimizu A., Tsukatani N., Tanikawa S., Fujita N., Harayama S.;
RT   "Comparison of the complete genome sequences of Rhodococcus erythropolis
RT   PR4 and Rhodococcus opacus B4.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AP008957; BAH34606.1; -; Genomic_DNA.
DR   RefSeq; WP_003945160.1; NC_012490.1.
DR   AlphaFoldDB; C1A1X1; -.
DR   SMR; C1A1X1; -.
DR   STRING; 234621.RER_38980; -.
DR   EnsemblBacteria; BAH34606; BAH34606; RER_38980.
DR   GeneID; 64141687; -.
DR   KEGG; rer:RER_38980; -.
DR   eggNOG; COG1637; Bacteria.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000002204; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..224
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000212716"
SQ   SEQUENCE   224 AA;  25019 MW;  AC6AC5F2F486F333 CRC64;
     MRLVIARCRV DYVGRLTSHL PTARRLLLVK ADGSVSIHAD DRAYKPLNWM SPPCWLVETT
     GDSDDILWVV TNKAGEELRI TLEEVEHDSS YELGLDPGLI KDGVEAHLQE LLAEHVETLG
     PGYTLVRREY MTAIGPVDLL VRDADGASVA VEIKRRGEID GVEQLTRYLE LLNRDPLLAP
     VTGVFAAQQI KPQAKTLAND RGIRCLVLDY EALRGTESTE FRLF