ID   NUCS_RHOJR              Reviewed;         224 AA.
AC   Q0SGQ9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722};
GN   OrderedLocusNames=RHA1_ro01459;
OS   Rhodococcus jostii (strain RHA1).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus.
OX   NCBI_TaxID=101510;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RHA1;
RX   PubMed=17030794; DOI=10.1073/pnas.0607048103;
RA   McLeod M.P., Warren R.L., Hsiao W.W.L., Araki N., Myhre M., Fernandes C.,
RA   Miyazawa D., Wong W., Lillquist A.L., Wang D., Dosanjh M., Hara H.,
RA   Petrescu A., Morin R.D., Yang G., Stott J.M., Schein J.E., Shin H.,
RA   Smailus D., Siddiqui A.S., Marra M.A., Jones S.J.M., Holt R.,
RA   Brinkman F.S.L., Miyauchi K., Fukuda M., Davies J.E., Mohn W.W.,
RA   Eltis L.D.;
RT   "The complete genome of Rhodococcus sp. RHA1 provides insights into a
RT   catabolic powerhouse.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15582-15587(2006).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000431; ABG93277.1; -; Genomic_DNA.
DR   RefSeq; WP_005248359.1; NC_008268.1.
DR   AlphaFoldDB; Q0SGQ9; -.
DR   SMR; Q0SGQ9; -.
DR   STRING; 101510.RHA1_ro01459; -.
DR   EnsemblBacteria; ABG93277; ABG93277; RHA1_ro01459.
DR   GeneID; 66787979; -.
DR   KEGG; rha:RHA1_ro01459; -.
DR   eggNOG; COG1637; Bacteria.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000008710; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..224
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000045835"
SQ   SEQUENCE   224 AA;  24955 MW;  9DE31E4A41613E2D CRC64;
     MRLVIARCRV DYVGRLTAHL PTARRLLLIK ADGSVSIHAD DRAYKPLNWM SPPCWLVEDT
     VEDAEALWVV TNKAGEELRI TIEEIEHDSS HELGVDPGLV KDGVEAHLQE LLAEHVETLG
     SGYTLVRREY MTAIGPVDLL CRNADGASVA VEIKRRGDID GVEQLTRYLE LLNRDPLLAP
     VSGVFAAQQI KPQAKTLATD RGIRCLVLDY EALRGTESTE FRLF