ID   NUCS_SACS2              Reviewed;         236 AA.
AC   Q97WK8;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=SSO2208;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; AE006641; AAK42378.1; -; Genomic_DNA.
DR   PIR; C90390; C90390.
DR   RefSeq; WP_009992078.1; NC_002754.1.
DR   AlphaFoldDB; Q97WK8; -.
DR   SMR; Q97WK8; -.
DR   STRING; 273057.SSO2208; -.
DR   EnsemblBacteria; AAK42378; AAK42378; SSO2208.
DR   GeneID; 44127943; -.
DR   KEGG; sso:SSO2208; -.
DR   PATRIC; fig|273057.12.peg.2304; -.
DR   eggNOG; arCOG01304; Archaea.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   InParanoid; Q97WK8; -.
DR   OMA; RCSVDYA; -.
DR   PhylomeDB; Q97WK8; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..236
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155700"
SQ   SEQUENCE   236 AA;  27265 MW;  6930125F102BBAC8 CRC64;
     MYSVLLNPSN TEIYSFLTDR IYRELIVIFA TCKVNYKGRA ESVASESPRL IILKPDGTVI
     IHESVKREPL NWQPPGTKIE IMNDYPLKIV AQRNRPKEVI EIDLKEVFYI TSAEVKEGDF
     TIKGREIDIV NTIIQNPSMI EEGFVPLTRE YNTPYGKIDL IGLDKKGNFV IIEVKRSKAQ
     LNAISQLYRY YLHMREIKGD KIRGILVAPD LTIHARELLQ KLGLEFIRYD IKNYSS