ID   NUCS_STRCO              Reviewed;         223 AA.
AC   Q9K4C3;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=SCO5388;
GN   ORFNames=2SC6G5.32c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB94559.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL939123; CAB94559.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_629527.1; NC_003888.3.
DR   RefSeq; WP_003973608.1; NC_003888.3.
DR   RefSeq; WP_007387684.1; NZ_VNID01000011.1.
DR   AlphaFoldDB; Q9K4C3; -.
DR   SMR; Q9K4C3; -.
DR   STRING; 100226.SCO5388; -.
DR   GeneID; 1100828; -.
DR   KEGG; sco:SCO5388; -.
DR   PATRIC; fig|100226.15.peg.5469; -.
DR   eggNOG; COG1637; Bacteria.
DR   HOGENOM; CLU_069350_0_0_11; -.
DR   InParanoid; Q9K4C3; -.
DR   PhylomeDB; Q9K4C3; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..223
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155687"
SQ   SEQUENCE   223 AA;  24601 MW;  67632C365191290A CRC64;
     MRLVIARCSV DYAGRLTAHL PSAPRLILVK ADGSVSIHAD DRAYKPLNWM SPPCALKEGT
     GEEEGVWTVV NKAGEKLIIT MEEILHDSSH ELGVDPGLIK DGVEAHLQEL LADRIDTLGE
     GYTLIRREYM TAIGPVDILC RDAQGGTVAV EIKRRGEIDG VEQLTRYLEL LNRDPHLAPV
     RGVFAAQEIK PQARVLATDR GIGCQVLDYD ALRGIEDDKL RLF