ID   NUCS_SULAC              Reviewed;         250 AA.
AC   Q4JC60;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=Saci_0200;
OS   Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC
OS   15157 / NCIMB 11770).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=330779;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770;
RX   PubMed=15995215; DOI=10.1128/jb.187.14.4992-4999.2005;
RA   Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E.,
RA   Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.;
RT   "The genome of Sulfolobus acidocaldarius, a model organism of the
RT   Crenarchaeota.";
RL   J. Bacteriol. 187:4992-4999(2005).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP000077; AAY79619.1; -; Genomic_DNA.
DR   RefSeq; WP_011277120.1; NC_007181.1.
DR   AlphaFoldDB; Q4JC60; -.
DR   SMR; Q4JC60; -.
DR   STRING; 330779.Saci_0200; -.
DR   EnsemblBacteria; AAY79619; AAY79619; Saci_0200.
DR   GeneID; 3474013; -.
DR   KEGG; sai:Saci_0200; -.
DR   PATRIC; fig|330779.12.peg.192; -.
DR   eggNOG; arCOG01304; Archaea.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   OMA; RCSVDYA; -.
DR   Proteomes; UP000001018; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..250
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155699"
SQ   SEQUENCE   250 AA;  28418 MW;  8FD14BE893CC1679 CRC64;
     MFKVLLEPDL QEALIFLNES VNALLTIYSE CEILYSGRAK SRASLSPRLT IIKPDGSVII
     HGPTKREPVN WQPPGSRIEY SIESGVLTVN AERKRPKERL SILHHRVYYI TSSEVKPGEF
     FLVGREKDEV DFIINNPDVI EGGFKPIHRE YRTPYGTVDL IGKDKEGNLV VLEFKRAKAS
     LQAVSQLYRY IMYFKEIGEN ARGILVAPGI SENALNLLKR LELEYVNISD KLGDSTISRP
     INYVPNLQRD