ID   NUCS_SULIL              Reviewed;         237 AA.
AC   C3MJA1;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=LS215_0025;
OS   Sulfolobus islandicus (strain L.S.2.15 / Lassen #1).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfolobus.
OX   NCBI_TaxID=429572;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L.S.2.15 / Lassen #1;
RX   PubMed=19435847; DOI=10.1073/pnas.0808945106;
RA   Reno M.L., Held N.L., Fields C.J., Burke P.V., Whitaker R.J.;
RT   "Biogeography of the Sulfolobus islandicus pan-genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:8605-8610(2009).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; CP001399; ACP34179.1; -; Genomic_DNA.
DR   RefSeq; WP_012710204.1; NC_012589.1.
DR   AlphaFoldDB; C3MJA1; -.
DR   SMR; C3MJA1; -.
DR   EnsemblBacteria; ACP34179; ACP34179; LS215_0025.
DR   GeneID; 7812085; -.
DR   GeneID; 7939724; -.
DR   GeneID; 8759864; -.
DR   KEGG; sis:LS215_0025; -.
DR   HOGENOM; CLU_069350_1_0_2; -.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 96363at2157; -.
DR   Proteomes; UP000001747; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011335; Restrct_endonuc-II-like.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
DR   SUPFAM; SSF52980; SSF52980; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease.
FT   CHAIN           1..237
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_1000212718"
SQ   SEQUENCE   237 AA;  27401 MW;  EC53D749733EDB84 CRC64;
     MYSVLLNPSN TEIYSFLTER IYRELVVIFA TCKVNYKGRA ESVASESPRL IILKPDGTVI
     IHESVKREPL NWQPPGTKIE IMNDYPLKIV AQRKRPKEVI EIDLKEVFYI TSAEVKDGDF
     VIKGREIDIV NTIIQNPSMI EEGFVPLTRE YNTPYGKIDL VGLDKKGNFV IIEVKRSKAQ
     LSAVSQLYRY YLHIRETKED KVRGILVAPD LTTHARELLQ KLGLEFIRYD IQKYSSY