ID   NUCS_SULTO              Reviewed;         252 AA.
AC   Q96Y77;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN   Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=STK_22900;
OS   Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
OS   (Sulfolobus tokodaii).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Sulfurisphaera.
OX   NCBI_TaxID=273063;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7;
RX   PubMed=11572479; DOI=10.1093/dnares/8.4.123;
RA   Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M.,
RA   Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y.,
RA   Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T.,
RA   Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K.,
RA   Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.;
RT   "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon,
RT   Sulfolobus tokodaii strain7.";
RL   DNA Res. 8:123-140(2001).
CC   -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC       structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC   -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC       {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR   EMBL; BA000023; BAB67400.1; -; Genomic_DNA.
DR   RefSeq; WP_010980375.1; NC_003106.2.
DR   AlphaFoldDB; Q96Y77; -.
DR   SMR; Q96Y77; -.
DR   STRING; 273063.STK_22900; -.
DR   EnsemblBacteria; BAB67400; BAB67400; STK_22900.
DR   GeneID; 1460373; -.
DR   KEGG; sto:STK_22900; -.
DR   PATRIC; fig|273063.9.peg.2597; -.
DR   eggNOG; arCOG01304; Archaea.
DR   OMA; RCSVDYA; -.
DR   OrthoDB; 96363at2157; -.
DR   Proteomes; UP000001015; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   HAMAP; MF_00722; NucS; 1.
DR   InterPro; IPR002793; Endonuclease_NucS.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   PANTHER; PTHR38814; PTHR38814; 1.
DR   Pfam; PF01939; NucS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW   Reference proteome.
FT   CHAIN           1..252
FT                   /note="Endonuclease NucS"
FT                   /id="PRO_0000155701"
SQ   SEQUENCE   252 AA;  29060 MW;  248855E7FE5AB583 CRC64;
     MTQVLLNPSL EEAYHFLVKN MYNNLITLFG DCEIQYEGRA YSHASLASRL IIIKVDGSVI
     VHEHTKREPI NWQPPGSVIV VRKESGVLSI ESIRKRPKER LYIILRRLYY LTSAEVNSGN
     FDIKGTEKDI VDLVLENPNL IEEGFKPIQR EYRIPYGIVD LFGYDKFGTP FVLEFKRSKA
     TLQAVSQLHR YYMFFLESYG KARGALVSPG ISEKALNLIE KLRLEYIDAN RIFDSITNSS
     RYTINISNIQ RS