NUCS_THEKO
ID NUCS_THEKO Reviewed; 252 AA.
AC Q5JER9;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Endonuclease NucS {ECO:0000255|HAMAP-Rule:MF_00722};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00722};
GN Name=nucS {ECO:0000255|HAMAP-Rule:MF_00722}; OrderedLocusNames=TK1898;
OS Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS (Pyrococcus kodakaraensis (strain KOD1)).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Thermococcus.
OX NCBI_TaxID=69014;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX PubMed=15710748; DOI=10.1101/gr.3003105;
RA Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL Genome Res. 15:352-363(2005).
CC -!- FUNCTION: Cleaves both 3' and 5' ssDNA extremities of branched DNA
CC structures. {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00722}.
CC -!- SIMILARITY: Belongs to the NucS endonuclease family.
CC {ECO:0000255|HAMAP-Rule:MF_00722}.
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DR EMBL; AP006878; BAD86087.1; -; Genomic_DNA.
DR PDB; 5GKE; X-ray; 2.40 A; A/B=1-252.
DR PDB; 5GKF; X-ray; 2.80 A; A/B=1-252.
DR PDB; 5GKG; X-ray; 2.60 A; A/B=1-252.
DR PDB; 5GKH; X-ray; 2.90 A; A/B=1-252.
DR PDB; 5GKI; X-ray; 2.90 A; A/B=1-252.
DR PDB; 5GKJ; X-ray; 3.20 A; A/B=1-252.
DR PDBsum; 5GKE; -.
DR PDBsum; 5GKF; -.
DR PDBsum; 5GKG; -.
DR PDBsum; 5GKH; -.
DR PDBsum; 5GKI; -.
DR PDBsum; 5GKJ; -.
DR AlphaFoldDB; Q5JER9; -.
DR SMR; Q5JER9; -.
DR STRING; 69014.TK1898; -.
DR EnsemblBacteria; BAD86087; BAD86087; TK1898.
DR KEGG; tko:TK1898; -.
DR PATRIC; fig|69014.16.peg.1856; -.
DR eggNOG; arCOG01304; Archaea.
DR HOGENOM; CLU_069350_1_0_2; -.
DR InParanoid; Q5JER9; -.
DR OMA; RCSVDYA; -.
DR PhylomeDB; Q5JER9; -.
DR BRENDA; 3.1.21.B3; 5246.
DR Proteomes; UP000000536; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000014; F:single-stranded DNA endodeoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1350.10; -; 1.
DR HAMAP; MF_00722; NucS; 1.
DR InterPro; IPR002793; Endonuclease_NucS.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR PANTHER; PTHR38814; PTHR38814; 1.
DR Pfam; PF01939; NucS; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome.
FT CHAIN 1..252
FT /note="Endonuclease NucS"
FT /id="PRO_0000155698"
FT STRAND 4..11
FT /evidence="ECO:0007829|PDB:5GKE"
FT HELIX 14..27
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 30..58
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 64..67
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 69..73
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 86..94
FT /evidence="ECO:0007829|PDB:5GKE"
FT TURN 99..102
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 103..118
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 127..130
FT /evidence="ECO:0007829|PDB:5GKE"
FT HELIX 132..141
FT /evidence="ECO:0007829|PDB:5GKE"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 151..158
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 160..169
FT /evidence="ECO:0007829|PDB:5GKE"
FT TURN 171..173
FT /evidence="ECO:0007829|PDB:5GKJ"
FT STRAND 175..180
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:5GKF"
FT HELIX 187..204
FT /evidence="ECO:0007829|PDB:5GKE"
FT HELIX 205..207
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 208..215
FT /evidence="ECO:0007829|PDB:5GKE"
FT HELIX 219..227
FT /evidence="ECO:0007829|PDB:5GKE"
FT STRAND 231..234
FT /evidence="ECO:0007829|PDB:5GKE"
SQ SEQUENCE 252 AA; 28595 MW; A9F0F53540EBBF7D CRC64;
MSKDKVTVIT SPSTEELVSL VNSALLEEAM LTIFARCKVH YDGRAKSELG SGDRVIIVKP
DGSFLIHQSK KREPVNWQPP GSRVRLELRE NPVLVSIRRK PRETLEVELE EVYMVSVFRA
EDYEELALTG SEAEMAELIF ENPEVIEPGF KPLFREKAIG TGIVDVLGRD SDGNIVVLEL
KRRRAELHAV RQLKSYVEIL REEYGDKVRG ILVAPSLTSG AKRLLEKEGL EFRKLEPPKR
DSKKKGRQKT LF
