ID   NUC_SHIFL               Reviewed;         174 AA.
AC   P29769;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Micrococcal nuclease;
DE            EC=3.1.31.1;
DE   Flags: Precursor;
GN   Name=nuc;
OS   Shigella flexneri.
OG   Plasmid IncW pSa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1560781; DOI=10.1111/j.1365-2958.1992.tb01497.x;
RA   Close S.M., Kado C.I.;
RT   "A gene near the plasmid pSa origin of replication encodes a nuclease.";
RL   Mol. Microbiol. 6:521-527(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-
CC         phosphooligonucleotide end-products.; EC=3.1.31.1;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10048, ECO:0000255|PROSITE-
CC         ProRule:PRU10049};
CC   -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00272}.
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DR   EMBL; U30471; AAA75246.1; -; Genomic_DNA.
DR   PIR; S20541; NCEBPX.
DR   AlphaFoldDB; P29769; -.
DR   SMR; P29769; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 2.40.50.90; -; 1.
DR   InterPro; IPR035437; SNase_OB-fold_sf.
DR   InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR   InterPro; IPR002071; Thermonucl_AS.
DR   Pfam; PF00565; SNase; 1.
DR   SMART; SM00318; SNc; 1.
DR   SUPFAM; SSF50199; SSF50199; 1.
DR   PROSITE; PS01123; TNASE_1; 1.
DR   PROSITE; PS01284; TNASE_2; 1.
DR   PROSITE; PS50830; TNASE_3; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..174
FT                   /note="Micrococcal nuclease"
FT                   /id="PRO_0000034394"
FT   ACT_SITE        52
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        60
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        94
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   174 AA;  19689 MW;  9B2B37729E4F30D7 CRC64;
     MKSALAALRA VAAAVVLIVS VPAWADFRGE VVRILDGDTI DVLVNRQTIR VRLADIDAPE
     SGQAFGSRAR QRLADLTFRQ EVQVTEKEVD RYGRTLGVVY APLQYPGGQT QLTNINAIMV
     QEGMAWAYRY YGKPTDAQMY EYEKEARRQR LGLWSDPNAQ EPWKWRRASK NATN