NUC_STAAU
ID NUC_STAAU Reviewed; 231 AA.
AC P00644; P13718; Q53711;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 03-AUG-2022, entry version 189.
DE RecName: Full=Thermonuclease;
DE Short=TNase;
DE EC=3.1.31.1;
DE AltName: Full=Micrococcal nuclease;
DE AltName: Full=Staphylococcal nuclease {ECO:0000303|PubMed:4324890};
DE Contains:
DE RecName: Full=Nuclease B {ECO:0000303|PubMed:893427};
DE Contains:
DE RecName: Full=Nuclease A {ECO:0000303|PubMed:893427};
DE Flags: Precursor;
GN Name=nuc {ECO:0000303|PubMed:6307819};
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 27735 / Foggie;
RX PubMed=6307819; DOI=10.1016/0378-1119(83)90102-6;
RA Shortle D.;
RT "A genetic system for analysis of staphylococcal nuclease.";
RL Gene 22:181-189(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RX PubMed=3921523; DOI=10.1128/jb.162.2.521-528.1985;
RA Kovacevic S., Veal L.E., Hsiung H.M., Miller J.R.;
RT "Secretion of staphylococcal nuclease by Bacillus subtilis.";
RL J. Bacteriol. 162:521-528(1985).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 30-63.
RX PubMed=3112123; DOI=10.1128/jb.169.8.3508-3514.1987;
RA Miller J.R., Kovacevic S., Veal L.E.;
RT "Secretion and processing of staphylococcal nuclease by Bacillus
RT subtilis.";
RL J. Bacteriol. 169:3508-3514(1987).
RN [4]
RP PROTEIN SEQUENCE OF 64-82, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 27735 / Foggie;
RX PubMed=893427; DOI=10.1016/s0021-9258(17)39992-1;
RA Davis A., Moore I.B., Parker D.S., Taniuchi H.;
RT "Nuclease B. A possible precursor of nuclease A, an extracellular nuclease
RT of Staphylococcus aureus.";
RL J. Biol. Chem. 252:6544-6553(1977).
RN [5]
RP PROTEIN SEQUENCE OF 83-231, AND SUBCELLULAR LOCATION.
RC STRAIN=ATCC 27735 / Foggie;
RX PubMed=4324890; DOI=10.1016/s0021-9258(18)62201-x;
RA Cone J.L., Cusumano C.L., Taniuchi H., Anfinsen C.B.;
RT "Staphylococcal nuclease (Foggi strain). II. The amino acid sequence.";
RL J. Biol. Chem. 246:3103-3110(1971).
RN [6]
RP AMIDE ASSIGNMENTS FOR 83-231.
RC STRAIN=ATCC 27735 / Foggie;
RX PubMed=4339720; DOI=10.1016/s0021-9258(19)45023-0;
RA Bohnert J.L., Taniuchi H.;
RT "The examination of the presence of amide groups in glutamic acid and
RT aspartic acid residues of staphylococcal nuclease (Foggi strain).";
RL J. Biol. Chem. 247:4557-4560(1972).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS), ACTIVE SITE, AND PROPOSED CATALYTIC
RP MECHANISM.
RX PubMed=288045; DOI=10.1073/pnas.76.6.2551;
RA Cotton F.A., Hazen E.C. Jr., Legg M.J.;
RT "Staphylococcal nuclease: proposed mechanism of action based on structure
RT of enzyme-thymidine 3',5'-bisphosphate-calcium ion complex at 1.5-A
RT resolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 76:2551-2555(1979).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
RX PubMed=1896431; DOI=10.1002/prot.340100203;
RA Hynes T.R., Fox R.O.;
RT "The crystal structure of staphylococcal nuclease refined at 1.7-A
RT resolution.";
RL Proteins 10:92-105(1991).
RN [9]
RP STRUCTURE BY NMR.
RX PubMed=1731948; DOI=10.1021/bi00118a038;
RA Wang J., Mooberry E.S., Walkenhorst W.F., Markley J.L.;
RT "Solution studies of staphylococcal nuclease H124L. 1. Backbone 1H and 15N
RT resonances and secondary structure of the unligated enzyme as identified by
RT three-dimensional NMR spectroscopy.";
RL Biochemistry 31:911-920(1992).
RN [10]
RP STRUCTURE BY NMR OF 83-218.
RX PubMed=8069625; DOI=10.1016/0969-2126(93)90027-e;
RA Shortle D., Abeygunawardana C.;
RT "NMR analysis of the residual structure in the denatured state of an
RT unusual mutant of staphylococcal nuclease.";
RL Structure 1:121-134(1993).
CC -!- FUNCTION: Enzyme that catalyzes the hydrolysis of both DNA and RNA at
CC the 5' position of the phosphodiester bond.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-
CC phosphooligonucleotide end-products.; EC=3.1.31.1;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10048, ECO:0000255|PROSITE-
CC ProRule:PRU10049};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Note=Binds 1 Ca(2+) ion per subunit.;
CC -!- SUBCELLULAR LOCATION: [Nuclease A]: Secreted
CC {ECO:0000269|PubMed:893427, ECO:0000305|PubMed:4324890}.
CC -!- SUBCELLULAR LOCATION: [Nuclease B]: Membrane.
CC -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC ProRule:PRU00272}.
CC -!- WEB RESOURCE: Name=Worthington enzyme manual;
CC URL="https://www.worthington-biochem.com/NFCP/";
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; V01281; CAA24594.1; -; mRNA.
DR EMBL; M17123; AAA26659.1; -; Genomic_DNA.
DR PIR; A00790; NCSAF.
DR PDB; 1A2T; X-ray; 1.96 A; A=83-231.
DR PDB; 1A2U; X-ray; 2.00 A; A=83-231.
DR PDB; 1A3T; X-ray; 2.10 A; A=83-231.
DR PDB; 1A3U; X-ray; 2.05 A; A=83-231.
DR PDB; 1A3V; X-ray; 2.20 A; A=83-231.
DR PDB; 1AEX; X-ray; 2.10 A; A=83-231.
DR PDB; 1ENA; X-ray; 2.15 A; A=89-223.
DR PDB; 1ENC; X-ray; 1.95 A; A=83-231.
DR PDB; 1EQV; X-ray; 1.94 A; A=88-223.
DR PDB; 1EY0; X-ray; 1.60 A; A=83-231.
DR PDB; 1EY4; X-ray; 1.60 A; A=83-231.
DR PDB; 1EY5; X-ray; 1.70 A; A=83-231.
DR PDB; 1EY6; X-ray; 1.75 A; A=83-231.
DR PDB; 1EY7; X-ray; 1.88 A; A=83-231.
DR PDB; 1EY8; X-ray; 1.75 A; A=83-231.
DR PDB; 1EY9; X-ray; 1.72 A; A=83-231.
DR PDB; 1EYA; X-ray; 2.00 A; A=83-231.
DR PDB; 1EYC; X-ray; 1.85 A; A=83-231.
DR PDB; 1EYD; X-ray; 1.70 A; A=83-231.
DR PDB; 1EZ6; X-ray; 1.90 A; A=83-231.
DR PDB; 1EZ8; X-ray; 1.85 A; A=83-231.
DR PDB; 1F2M; X-ray; 2.00 A; A=83-231.
DR PDB; 1F2Y; X-ray; 2.00 A; A=83-231.
DR PDB; 1F2Z; X-ray; 1.80 A; A=83-231.
DR PDB; 1IHZ; X-ray; 1.65 A; A=83-231.
DR PDB; 1II3; X-ray; 1.72 A; A=83-231.
DR PDB; 1JOK; NMR; -; A=83-231.
DR PDB; 1JOO; NMR; -; A=83-231.
DR PDB; 1JOQ; NMR; -; A=83-231.
DR PDB; 1JOR; NMR; -; A=83-231.
DR PDB; 1KAA; X-ray; 1.90 A; A=88-223.
DR PDB; 1KAB; X-ray; 1.80 A; A=88-223.
DR PDB; 1KDA; X-ray; 1.90 A; A=83-231.
DR PDB; 1KDB; X-ray; 1.90 A; A=83-231.
DR PDB; 1KDC; X-ray; 2.00 A; A=83-231.
DR PDB; 1NSN; X-ray; 2.80 A; S=83-231.
DR PDB; 1NUC; X-ray; 1.90 A; A=83-231.
DR PDB; 1RKN; NMR; -; A=83-192.
DR PDB; 1SNC; X-ray; 1.65 A; A=83-231.
DR PDB; 1SND; X-ray; 1.84 A; A/B=83-231.
DR PDB; 1SNM; X-ray; 1.74 A; A=83-231.
DR PDB; 1SNO; X-ray; 1.70 A; A=83-231.
DR PDB; 1SNP; X-ray; 1.95 A; A=83-231.
DR PDB; 1SNQ; X-ray; 1.70 A; A=83-231.
DR PDB; 1STA; X-ray; 1.70 A; A=83-231.
DR PDB; 1STB; X-ray; 2.00 A; A=83-230.
DR PDB; 1STG; X-ray; 1.70 A; A=83-231.
DR PDB; 1STH; X-ray; 1.85 A; A=83-231.
DR PDB; 1STN; X-ray; 1.70 A; A=83-231.
DR PDB; 1STY; X-ray; 1.67 A; A=83-231.
DR PDB; 1SYB; X-ray; 1.80 A; A=83-231.
DR PDB; 1SYC; X-ray; 1.80 A; A=83-231.
DR PDB; 1SYD; X-ray; 1.70 A; A=83-231.
DR PDB; 1SYE; X-ray; 1.80 A; A=83-231.
DR PDB; 1SYF; X-ray; 1.80 A; A=83-231.
DR PDB; 1SYG; X-ray; 1.90 A; A=83-231.
DR PDB; 1TQO; X-ray; 2.00 A; A=83-226.
DR PDB; 1TR5; X-ray; 2.10 A; A=83-226.
DR PDB; 1TT2; X-ray; 1.85 A; A=83-226.
DR PDB; 1U9R; X-ray; 2.10 A; A=83-231.
DR PDB; 2ENB; X-ray; 2.05 A; A=89-223.
DR PDB; 2EXZ; X-ray; 1.90 A; A=83-231.
DR PDB; 2EY1; X-ray; 1.85 A; A=83-231.
DR PDB; 2EY2; X-ray; 1.70 A; A=83-231.
DR PDB; 2EY5; X-ray; 2.00 A; A=83-231.
DR PDB; 2EY6; X-ray; 1.65 A; A=83-231.
DR PDB; 2EYF; X-ray; 1.80 A; A=83-231.
DR PDB; 2EYH; X-ray; 1.90 A; A=83-231.
DR PDB; 2EYJ; X-ray; 1.85 A; A=83-231.
DR PDB; 2EYL; X-ray; 1.70 A; A=83-231.
DR PDB; 2EYM; X-ray; 1.80 A; A=83-231.
DR PDB; 2EYO; X-ray; 1.70 A; A=83-231.
DR PDB; 2EYP; X-ray; 1.80 A; A=83-231.
DR PDB; 2F0D; X-ray; 2.50 A; A=83-231.
DR PDB; 2F0E; X-ray; 1.95 A; A=83-231.
DR PDB; 2F0F; X-ray; 2.15 A; A=83-231.
DR PDB; 2F0G; X-ray; 1.90 A; A=83-231.
DR PDB; 2F0H; X-ray; 2.15 A; A=83-231.
DR PDB; 2F0I; X-ray; 1.80 A; A=83-231.
DR PDB; 2F0J; X-ray; 1.80 A; A=83-231.
DR PDB; 2F0K; X-ray; 1.90 A; A=83-231.
DR PDB; 2F0L; X-ray; 1.90 A; A=83-231.
DR PDB; 2F0M; X-ray; 2.00 A; A=83-231.
DR PDB; 2F0N; X-ray; 1.70 A; A=83-231.
DR PDB; 2F0O; X-ray; 1.90 A; A=83-231.
DR PDB; 2F0P; X-ray; 2.05 A; A=83-231.
DR PDB; 2F0Q; X-ray; 1.95 A; A=83-231.
DR PDB; 2F0S; X-ray; 1.75 A; A=83-231.
DR PDB; 2F0T; X-ray; 1.70 A; A=83-231.
DR PDB; 2F0U; X-ray; 1.83 A; A=83-231.
DR PDB; 2F0V; X-ray; 2.00 A; A=83-231.
DR PDB; 2F0W; X-ray; 2.10 A; A=83-231.
DR PDB; 2F3V; NMR; -; A=83-192.
DR PDB; 2F3W; NMR; -; A=83-192.
DR PDB; 2FXY; NMR; -; A=137-154.
DR PDB; 2FXZ; NMR; -; A=179-191.
DR PDB; 2GSI; X-ray; 2.81 A; W/X/Y/Z=126-136.
DR PDB; 2KHS; NMR; -; A=83-203.
DR PDB; 2NUC; X-ray; 2.00 A; A=83-231.
DR PDB; 2OEO; X-ray; 2.00 A; A=83-226.
DR PDB; 2OF1; X-ray; 1.92 A; A=83-226.
DR PDB; 2PQE; NMR; -; A=83-231.
DR PDB; 2QDB; X-ray; 2.20 A; A=83-231.
DR PDB; 2RBM; X-ray; 1.90 A; A=83-231.
DR PDB; 2RDF; X-ray; 2.01 A; A=83-231.
DR PDB; 2RKS; X-ray; 2.01 A; A=83-231.
DR PDB; 2SNM; X-ray; 1.97 A; A=83-231.
DR PDB; 2SNS; X-ray; 1.50 A; A=83-231.
DR PDB; 2SOB; NMR; -; A=83-185.
DR PDB; 3BDC; X-ray; 1.80 A; A=83-231.
DR PDB; 3C1E; X-ray; 1.90 A; A=83-231.
DR PDB; 3C1F; X-ray; 2.00 A; A=83-231.
DR PDB; 3D4D; X-ray; 2.10 A; A/B=83-231.
DR PDB; 3D6C; X-ray; 2.00 A; A=83-231.
DR PDB; 3DHQ; X-ray; 2.15 A; A=83-231.
DR PDB; 3DMU; X-ray; 1.80 A; A=83-231.
DR PDB; 3E5S; X-ray; 2.00 A; A/B=83-231.
DR PDB; 3EJI; X-ray; 1.90 A; A=83-231.
DR PDB; 3ERO; X-ray; 1.85 A; A=83-231.
DR PDB; 3ERQ; X-ray; 2.10 A; A=83-231.
DR PDB; 3EVQ; X-ray; 2.00 A; A=83-231.
DR PDB; 3H6M; X-ray; 1.70 A; A=83-231.
DR PDB; 3HEJ; X-ray; 1.80 A; A/B/C/D=83-231.
DR PDB; 3HZX; X-ray; 2.00 A; A=83-231.
DR PDB; 3ITP; X-ray; 1.75 A; A=83-231.
DR PDB; 3LX0; X-ray; 1.50 A; A=83-231.
DR PDB; 3MEH; X-ray; 1.50 A; A=83-231.
DR PDB; 3MHB; X-ray; 1.55 A; A=83-231.
DR PDB; 3MXP; X-ray; 1.61 A; A=83-231.
DR PDB; 3MZ5; X-ray; 1.58 A; A=83-231.
DR PDB; 3NHH; X-ray; 2.00 A; A=83-231.
DR PDB; 3NK9; X-ray; 1.65 A; A=83-231.
DR PDB; 3NP8; X-ray; 1.70 A; A=83-231.
DR PDB; 3NQT; X-ray; 1.90 A; A=83-231.
DR PDB; 3NUC; X-ray; 1.90 A; A=83-231.
DR PDB; 3NXW; X-ray; 1.65 A; A=83-231.
DR PDB; 3OSO; X-ray; 1.60 A; A=83-231.
DR PDB; 3OWF; X-ray; 1.85 A; A=83-231.
DR PDB; 3P1H; X-ray; 1.79 A; A=83-231.
DR PDB; 3P75; X-ray; 1.90 A; A=89-223.
DR PDB; 3PMF; X-ray; 1.60 A; A=83-231.
DR PDB; 3QB3; X-ray; 1.63 A; A=83-231.
DR PDB; 3RUZ; X-ray; 1.58 A; A=83-231.
DR PDB; 3S9W; X-ray; 1.90 A; A=83-231.
DR PDB; 3SHL; X-ray; 1.48 A; A=83-231.
DR PDB; 3SK4; X-ray; 1.85 A; A=83-231.
DR PDB; 3SK5; X-ray; 1.70 A; A=83-231.
DR PDB; 3SK6; X-ray; 1.80 A; A=83-231.
DR PDB; 3SK8; X-ray; 1.90 A; A=83-231.
DR PDB; 3SR1; X-ray; 1.45 A; A=83-231.
DR PDB; 3SXH; X-ray; 1.40 A; A=83-231.
DR PDB; 3T13; X-ray; 1.80 A; A/B=83-231.
DR PDB; 3T16; X-ray; 1.80 A; A=83-231.
DR PDB; 3TME; X-ray; 1.40 A; A=83-231.
DR PDB; 3TP5; X-ray; 1.80 A; A=83-231.
DR PDB; 3TP6; X-ray; 1.80 A; A=83-231.
DR PDB; 3TP7; X-ray; 1.90 A; A=83-231.
DR PDB; 3TP8; X-ray; 1.60 A; A=83-231.
DR PDB; 3U9O; X-ray; 1.95 A; A=83-231.
DR PDB; 3V2T; X-ray; 1.80 A; A=83-231.
DR PDB; 3VA5; X-ray; 1.55 A; A=83-231.
DR PDB; 4DF7; X-ray; 1.50 A; A=83-231.
DR PDB; 4DFA; X-ray; 1.40 A; A=83-231.
DR PDB; 4DGZ; X-ray; 1.47 A; A=83-231.
DR PDB; 4DU9; X-ray; 1.63 A; A=83-231.
DR PDB; 4E6I; X-ray; 2.00 A; A/B=83-231.
DR PDB; 4EQN; X-ray; 1.80 A; A=83-231.
DR PDB; 4EQO; X-ray; 1.70 A; A=83-231.
DR PDB; 4EQP; X-ray; 1.35 A; A=83-231.
DR PDB; 4EVO; X-ray; 1.95 A; A=83-231.
DR PDB; 4F7X; X-ray; 1.70 A; A=83-231.
DR PDB; 4F8M; X-ray; 1.60 A; A=83-231.
DR PDB; 4G57; X-ray; 1.81 A; A=89-223.
DR PDB; 4H7B; X-ray; 1.60 A; A=83-231.
DR PDB; 4HMI; X-ray; 1.75 A; A=83-231.
DR PDB; 4HMJ; X-ray; 1.35 A; A=83-231.
DR PDB; 4HTH; X-ray; 1.75 A; A=83-231.
DR PDB; 4I65; X-ray; 1.61 A; A=88-223.
DR PDB; 4IAL; X-ray; 1.60 A; A=83-231.
DR PDB; 4ID6; X-ray; 1.90 A; A=83-231.
DR PDB; 4IUN; X-ray; 1.60 A; A=83-231.
DR PDB; 4IZ8; X-ray; 1.70 A; A=83-231.
DR PDB; 4J1M; X-ray; 1.65 A; A=83-231.
DR PDB; 4J6H; X-ray; 1.60 A; A=83-231.
DR PDB; 4K14; X-ray; 1.60 A; A=88-223.
DR PDB; 4K2K; X-ray; 1.65 A; A=83-231.
DR PDB; 4K2L; X-ray; 1.55 A; A=83-231.
DR PDB; 4K5V; X-ray; 1.75 A; A=83-231.
DR PDB; 4K5W; X-ray; 1.65 A; A=83-231.
DR PDB; 4K5X; X-ray; 1.65 A; A=83-231.
DR PDB; 4K6D; X-ray; 1.65 A; A=83-231.
DR PDB; 4K8I; X-ray; 2.10 A; A=89-223.
DR PDB; 4K8J; X-ray; 2.00 A; A=89-223.
DR PDB; 4KD3; X-ray; 1.75 A; A=83-231.
DR PDB; 4KD4; X-ray; 1.55 A; A=83-231.
DR PDB; 4KHV; X-ray; 1.60 A; A=83-231.
DR PDB; 4KJN; X-ray; 1.55 A; A=83-231.
DR PDB; 4KJO; X-ray; 1.90 A; A=83-231.
DR PDB; 4KTA; X-ray; 1.95 A; A=83-231.
DR PDB; 4KV6; X-ray; 1.55 A; A=83-231.
DR PDB; 4KY5; X-ray; 1.40 A; A=83-231.
DR PDB; 4KY6; X-ray; 1.70 A; A=83-231.
DR PDB; 4KY7; X-ray; 1.55 A; A=83-231.
DR PDB; 4LAA; X-ray; 1.58 A; A=83-231.
DR PDB; 4ME5; X-ray; 1.80 A; A=83-231.
DR PDB; 4MIU; X-ray; 1.67 A; A=83-231.
DR PDB; 4N9P; X-ray; 1.40 A; A=83-231.
DR PDB; 4N9T; X-ray; 1.60 A; A=83-231.
DR PDB; 4NDX; X-ray; 1.50 A; A=83-231.
DR PDB; 4NKL; X-ray; 1.60 A; A=83-231.
DR PDB; 4NMZ; X-ray; 1.70 A; A=83-231.
DR PDB; 4NP5; X-ray; 1.50 A; A=83-231.
DR PDB; 4ODG; X-ray; 1.73 A; A=83-231.
DR PDB; 4OL7; X-ray; 1.67 A; A/B=83-231.
DR PDB; 4PMB; X-ray; 1.80 A; A=83-231.
DR PDB; 4PMC; X-ray; 1.65 A; A=83-231.
DR PDB; 4PNY; X-ray; 1.68 A; A=83-231.
DR PDB; 4QB4; X-ray; 1.60 A; A=88-223.
DR PDB; 4QF4; X-ray; 1.80 A; A/B=83-231.
DR PDB; 4R8N; X-ray; 1.65 A; A=83-223.
DR PDB; 4RKB; X-ray; 1.88 A; A=83-231.
DR PDB; 4RKL; X-ray; 1.66 A; A=83-231.
DR PDB; 4S3S; X-ray; 1.64 A; A=83-231.
DR PDB; 4TRD; X-ray; 1.60 A; A=83-231.
DR PDB; 4WOR; X-ray; 1.60 A; A=83-231.
DR PDB; 4YIJ; X-ray; 1.64 A; A/B=83-231.
DR PDB; 4ZQ3; X-ray; 1.90 A; A=83-231.
DR PDB; 4ZUI; X-ray; 1.70 A; A=83-231.
DR PDB; 4ZUJ; X-ray; 1.72 A; A=83-231.
DR PDB; 5C3W; X-ray; 1.72 A; A=83-231.
DR PDB; 5C3X; X-ray; 1.95 A; A=83-231.
DR PDB; 5C4H; X-ray; 1.80 A; A=83-231.
DR PDB; 5C4Z; X-ray; 1.62 A; A=83-231.
DR PDB; 5C5L; X-ray; 1.75 A; A=83-231.
DR PDB; 5C5M; X-ray; 1.70 A; A=83-231.
DR PDB; 5C6A; X-ray; 1.85 A; A=83-231.
DR PDB; 5CC4; X-ray; 1.90 A; A=83-231.
DR PDB; 5CGK; X-ray; 1.85 A; A=83-231.
DR PDB; 5CR3; X-ray; 1.80 A; A=83-231.
DR PDB; 5CV4; X-ray; 1.80 A; A=83-231.
DR PDB; 5CV5; X-ray; 1.80 A; A/B=83-231.
DR PDB; 5CV6; X-ray; 1.95 A; A=83-231.
DR PDB; 5CV7; X-ray; 1.65 A; A=83-231.
DR PDB; 5CV8; X-ray; 1.58 A; A=83-231.
DR PDB; 5CV9; X-ray; 1.62 A; A=83-231.
DR PDB; 5DEH; X-ray; 1.70 A; A=83-231.
DR PDB; 5E1F; X-ray; 1.90 A; A=83-231.
DR PDB; 5E3F; X-ray; 1.75 A; A=83-231.
DR PDB; 5EGT; X-ray; 1.85 A; A=83-231.
DR PDB; 5EKK; X-ray; 2.10 A; A=83-231.
DR PDB; 5EKL; X-ray; 2.10 A; A=83-231.
DR PDB; 5F2D; X-ray; 1.70 A; A=83-231.
DR PDB; 5HGT; X-ray; 1.75 A; A=83-231.
DR PDB; 5HUR; X-ray; 1.50 A; A=83-231.
DR PDB; 5I6W; X-ray; 1.90 A; A=83-231.
DR PDB; 5I6Y; X-ray; 1.90 A; A=83-231.
DR PDB; 5I9O; X-ray; 1.95 A; A/B=83-231.
DR PDB; 5I9P; X-ray; 1.85 A; A=83-231.
DR PDB; 5IGB; X-ray; 1.80 A; A=83-231.
DR PDB; 5IGC; X-ray; 1.80 A; A=83-231.
DR PDB; 5IGD; X-ray; 1.80 A; A=83-231.
DR PDB; 5IGE; X-ray; 1.80 A; A=83-231.
DR PDB; 5IGF; X-ray; 1.70 A; A=83-231.
DR PDB; 5IGG; X-ray; 1.70 A; A=83-231.
DR PDB; 5IIF; X-ray; 1.85 A; A=83-231.
DR PDB; 5IOC; X-ray; 1.90 A; A=83-231.
DR PDB; 5IOD; X-ray; 1.60 A; A=83-231.
DR PDB; 5ISR; X-ray; 1.90 A; A=83-231.
DR PDB; 5J1Z; X-ray; 1.73 A; A=83-231.
DR PDB; 5J22; X-ray; 1.90 A; A=83-231.
DR PDB; 5JAV; X-ray; 1.89 A; A=83-231.
DR PDB; 5JOB; X-ray; 1.45 A; A=83-231.
DR PDB; 5K5P; X-ray; 1.83 A; A=83-231.
DR PDB; 5KEE; X-ray; 1.50 A; A=83-231.
DR PDB; 5KGU; X-ray; 1.90 A; A=83-231.
DR PDB; 5KIX; X-ray; 1.75 A; A=83-231.
DR PDB; 5KRU; X-ray; 1.60 A; A=83-231.
DR PDB; 5KYI; X-ray; 1.70 A; A=83-231.
DR PDB; 5KYL; X-ray; 1.85 A; A=83-231.
DR PDB; 5NUC; X-ray; 2.10 A; A=83-231.
DR PDB; 6AMF; X-ray; 1.85 A; A=83-231.
DR PDB; 6B8R; X-ray; 1.65 A; A=83-231.
DR PDB; 6EEG; X-ray; 1.95 A; A=83-231.
DR PDB; 6EEK; X-ray; 2.20 A; A=83-231.
DR PDB; 6OK8; X-ray; 1.80 A; A=83-231.
DR PDB; 6U0W; X-ray; 1.90 A; A=83-231.
DR PDB; 6U0X; X-ray; 1.86 A; A=83-231.
DR PDB; 6XSB; X-ray; 1.95 A; A=83-231.
DR PDB; 6XSC; X-ray; 2.00 A; A=83-231.
DR PDB; 6XSE; X-ray; 2.10 A; A=83-231.
DR PDB; 6XSF; X-ray; 1.60 A; A=83-231.
DR PDB; 6XSG; X-ray; 2.00 A; A=83-231.
DR PDB; 6XSH; X-ray; 2.00 A; A=83-231.
DR PDB; 6XSI; X-ray; 2.00 A; A=83-231.
DR PDBsum; 1A2T; -.
DR PDBsum; 1A2U; -.
DR PDBsum; 1A3T; -.
DR PDBsum; 1A3U; -.
DR PDBsum; 1A3V; -.
DR PDBsum; 1AEX; -.
DR PDBsum; 1ENA; -.
DR PDBsum; 1ENC; -.
DR PDBsum; 1EQV; -.
DR PDBsum; 1EY0; -.
DR PDBsum; 1EY4; -.
DR PDBsum; 1EY5; -.
DR PDBsum; 1EY6; -.
DR PDBsum; 1EY7; -.
DR PDBsum; 1EY8; -.
DR PDBsum; 1EY9; -.
DR PDBsum; 1EYA; -.
DR PDBsum; 1EYC; -.
DR PDBsum; 1EYD; -.
DR PDBsum; 1EZ6; -.
DR PDBsum; 1EZ8; -.
DR PDBsum; 1F2M; -.
DR PDBsum; 1F2Y; -.
DR PDBsum; 1F2Z; -.
DR PDBsum; 1IHZ; -.
DR PDBsum; 1II3; -.
DR PDBsum; 1JOK; -.
DR PDBsum; 1JOO; -.
DR PDBsum; 1JOQ; -.
DR PDBsum; 1JOR; -.
DR PDBsum; 1KAA; -.
DR PDBsum; 1KAB; -.
DR PDBsum; 1KDA; -.
DR PDBsum; 1KDB; -.
DR PDBsum; 1KDC; -.
DR PDBsum; 1NSN; -.
DR PDBsum; 1NUC; -.
DR PDBsum; 1RKN; -.
DR PDBsum; 1SNC; -.
DR PDBsum; 1SND; -.
DR PDBsum; 1SNM; -.
DR PDBsum; 1SNO; -.
DR PDBsum; 1SNP; -.
DR PDBsum; 1SNQ; -.
DR PDBsum; 1STA; -.
DR PDBsum; 1STB; -.
DR PDBsum; 1STG; -.
DR PDBsum; 1STH; -.
DR PDBsum; 1STN; -.
DR PDBsum; 1STY; -.
DR PDBsum; 1SYB; -.
DR PDBsum; 1SYC; -.
DR PDBsum; 1SYD; -.
DR PDBsum; 1SYE; -.
DR PDBsum; 1SYF; -.
DR PDBsum; 1SYG; -.
DR PDBsum; 1TQO; -.
DR PDBsum; 1TR5; -.
DR PDBsum; 1TT2; -.
DR PDBsum; 1U9R; -.
DR PDBsum; 2ENB; -.
DR PDBsum; 2EXZ; -.
DR PDBsum; 2EY1; -.
DR PDBsum; 2EY2; -.
DR PDBsum; 2EY5; -.
DR PDBsum; 2EY6; -.
DR PDBsum; 2EYF; -.
DR PDBsum; 2EYH; -.
DR PDBsum; 2EYJ; -.
DR PDBsum; 2EYL; -.
DR PDBsum; 2EYM; -.
DR PDBsum; 2EYO; -.
DR PDBsum; 2EYP; -.
DR PDBsum; 2F0D; -.
DR PDBsum; 2F0E; -.
DR PDBsum; 2F0F; -.
DR PDBsum; 2F0G; -.
DR PDBsum; 2F0H; -.
DR PDBsum; 2F0I; -.
DR PDBsum; 2F0J; -.
DR PDBsum; 2F0K; -.
DR PDBsum; 2F0L; -.
DR PDBsum; 2F0M; -.
DR PDBsum; 2F0N; -.
DR PDBsum; 2F0O; -.
DR PDBsum; 2F0P; -.
DR PDBsum; 2F0Q; -.
DR PDBsum; 2F0S; -.
DR PDBsum; 2F0T; -.
DR PDBsum; 2F0U; -.
DR PDBsum; 2F0V; -.
DR PDBsum; 2F0W; -.
DR PDBsum; 2F3V; -.
DR PDBsum; 2F3W; -.
DR PDBsum; 2FXY; -.
DR PDBsum; 2FXZ; -.
DR PDBsum; 2GSI; -.
DR PDBsum; 2KHS; -.
DR PDBsum; 2NUC; -.
DR PDBsum; 2OEO; -.
DR PDBsum; 2OF1; -.
DR PDBsum; 2PQE; -.
DR PDBsum; 2QDB; -.
DR PDBsum; 2RBM; -.
DR PDBsum; 2RDF; -.
DR PDBsum; 2RKS; -.
DR PDBsum; 2SNM; -.
DR PDBsum; 2SNS; -.
DR PDBsum; 2SOB; -.
DR PDBsum; 3BDC; -.
DR PDBsum; 3C1E; -.
DR PDBsum; 3C1F; -.
DR PDBsum; 3D4D; -.
DR PDBsum; 3D6C; -.
DR PDBsum; 3DHQ; -.
DR PDBsum; 3DMU; -.
DR PDBsum; 3E5S; -.
DR PDBsum; 3EJI; -.
DR PDBsum; 3ERO; -.
DR PDBsum; 3ERQ; -.
DR PDBsum; 3EVQ; -.
DR PDBsum; 3H6M; -.
DR PDBsum; 3HEJ; -.
DR PDBsum; 3HZX; -.
DR PDBsum; 3ITP; -.
DR PDBsum; 3LX0; -.
DR PDBsum; 3MEH; -.
DR PDBsum; 3MHB; -.
DR PDBsum; 3MXP; -.
DR PDBsum; 3MZ5; -.
DR PDBsum; 3NHH; -.
DR PDBsum; 3NK9; -.
DR PDBsum; 3NP8; -.
DR PDBsum; 3NQT; -.
DR PDBsum; 3NUC; -.
DR PDBsum; 3NXW; -.
DR PDBsum; 3OSO; -.
DR PDBsum; 3OWF; -.
DR PDBsum; 3P1H; -.
DR PDBsum; 3P75; -.
DR PDBsum; 3PMF; -.
DR PDBsum; 3QB3; -.
DR PDBsum; 3RUZ; -.
DR PDBsum; 3S9W; -.
DR PDBsum; 3SHL; -.
DR PDBsum; 3SK4; -.
DR PDBsum; 3SK5; -.
DR PDBsum; 3SK6; -.
DR PDBsum; 3SK8; -.
DR PDBsum; 3SR1; -.
DR PDBsum; 3SXH; -.
DR PDBsum; 3T13; -.
DR PDBsum; 3T16; -.
DR PDBsum; 3TME; -.
DR PDBsum; 3TP5; -.
DR PDBsum; 3TP6; -.
DR PDBsum; 3TP7; -.
DR PDBsum; 3TP8; -.
DR PDBsum; 3U9O; -.
DR PDBsum; 3V2T; -.
DR PDBsum; 3VA5; -.
DR PDBsum; 4DF7; -.
DR PDBsum; 4DFA; -.
DR PDBsum; 4DGZ; -.
DR PDBsum; 4DU9; -.
DR PDBsum; 4E6I; -.
DR PDBsum; 4EQN; -.
DR PDBsum; 4EQO; -.
DR PDBsum; 4EQP; -.
DR PDBsum; 4EVO; -.
DR PDBsum; 4F7X; -.
DR PDBsum; 4F8M; -.
DR PDBsum; 4G57; -.
DR PDBsum; 4H7B; -.
DR PDBsum; 4HMI; -.
DR PDBsum; 4HMJ; -.
DR PDBsum; 4HTH; -.
DR PDBsum; 4I65; -.
DR PDBsum; 4IAL; -.
DR PDBsum; 4ID6; -.
DR PDBsum; 4IUN; -.
DR PDBsum; 4IZ8; -.
DR PDBsum; 4J1M; -.
DR PDBsum; 4J6H; -.
DR PDBsum; 4K14; -.
DR PDBsum; 4K2K; -.
DR PDBsum; 4K2L; -.
DR PDBsum; 4K5V; -.
DR PDBsum; 4K5W; -.
DR PDBsum; 4K5X; -.
DR PDBsum; 4K6D; -.
DR PDBsum; 4K8I; -.
DR PDBsum; 4K8J; -.
DR PDBsum; 4KD3; -.
DR PDBsum; 4KD4; -.
DR PDBsum; 4KHV; -.
DR PDBsum; 4KJN; -.
DR PDBsum; 4KJO; -.
DR PDBsum; 4KTA; -.
DR PDBsum; 4KV6; -.
DR PDBsum; 4KY5; -.
DR PDBsum; 4KY6; -.
DR PDBsum; 4KY7; -.
DR PDBsum; 4LAA; -.
DR PDBsum; 4ME5; -.
DR PDBsum; 4MIU; -.
DR PDBsum; 4N9P; -.
DR PDBsum; 4N9T; -.
DR PDBsum; 4NDX; -.
DR PDBsum; 4NKL; -.
DR PDBsum; 4NMZ; -.
DR PDBsum; 4NP5; -.
DR PDBsum; 4ODG; -.
DR PDBsum; 4OL7; -.
DR PDBsum; 4PMB; -.
DR PDBsum; 4PMC; -.
DR PDBsum; 4PNY; -.
DR PDBsum; 4QB4; -.
DR PDBsum; 4QF4; -.
DR PDBsum; 4R8N; -.
DR PDBsum; 4RKB; -.
DR PDBsum; 4RKL; -.
DR PDBsum; 4S3S; -.
DR PDBsum; 4TRD; -.
DR PDBsum; 4WOR; -.
DR PDBsum; 4YIJ; -.
DR PDBsum; 4ZQ3; -.
DR PDBsum; 4ZUI; -.
DR PDBsum; 4ZUJ; -.
DR PDBsum; 5C3W; -.
DR PDBsum; 5C3X; -.
DR PDBsum; 5C4H; -.
DR PDBsum; 5C4Z; -.
DR PDBsum; 5C5L; -.
DR PDBsum; 5C5M; -.
DR PDBsum; 5C6A; -.
DR PDBsum; 5CC4; -.
DR PDBsum; 5CGK; -.
DR PDBsum; 5CR3; -.
DR PDBsum; 5CV4; -.
DR PDBsum; 5CV5; -.
DR PDBsum; 5CV6; -.
DR PDBsum; 5CV7; -.
DR PDBsum; 5CV8; -.
DR PDBsum; 5CV9; -.
DR PDBsum; 5DEH; -.
DR PDBsum; 5E1F; -.
DR PDBsum; 5E3F; -.
DR PDBsum; 5EGT; -.
DR PDBsum; 5EKK; -.
DR PDBsum; 5EKL; -.
DR PDBsum; 5F2D; -.
DR PDBsum; 5HGT; -.
DR PDBsum; 5HUR; -.
DR PDBsum; 5I6W; -.
DR PDBsum; 5I6Y; -.
DR PDBsum; 5I9O; -.
DR PDBsum; 5I9P; -.
DR PDBsum; 5IGB; -.
DR PDBsum; 5IGC; -.
DR PDBsum; 5IGD; -.
DR PDBsum; 5IGE; -.
DR PDBsum; 5IGF; -.
DR PDBsum; 5IGG; -.
DR PDBsum; 5IIF; -.
DR PDBsum; 5IOC; -.
DR PDBsum; 5IOD; -.
DR PDBsum; 5ISR; -.
DR PDBsum; 5J1Z; -.
DR PDBsum; 5J22; -.
DR PDBsum; 5JAV; -.
DR PDBsum; 5JOB; -.
DR PDBsum; 5K5P; -.
DR PDBsum; 5KEE; -.
DR PDBsum; 5KGU; -.
DR PDBsum; 5KIX; -.
DR PDBsum; 5KRU; -.
DR PDBsum; 5KYI; -.
DR PDBsum; 5KYL; -.
DR PDBsum; 5NUC; -.
DR PDBsum; 6AMF; -.
DR PDBsum; 6B8R; -.
DR PDBsum; 6EEG; -.
DR PDBsum; 6EEK; -.
DR PDBsum; 6OK8; -.
DR PDBsum; 6U0W; -.
DR PDBsum; 6U0X; -.
DR PDBsum; 6XSB; -.
DR PDBsum; 6XSC; -.
DR PDBsum; 6XSE; -.
DR PDBsum; 6XSF; -.
DR PDBsum; 6XSG; -.
DR PDBsum; 6XSH; -.
DR PDBsum; 6XSI; -.
DR AlphaFoldDB; P00644; -.
DR BMRB; P00644; -.
DR SMR; P00644; -.
DR DrugBank; DB03654; S,S-Propylthiocysteine.
DR DrugBank; DB04205; Thymidine-3',5'-Diphosphate.
DR ABCD; P00644; 2 sequenced antibodies.
DR BRENDA; 3.1.31.1; 3352.
DR EvolutionaryTrace; P00644; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR Gene3D; 2.40.50.90; -; 1.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR InterPro; IPR002071; Thermonucl_AS.
DR Pfam; PF00565; SNase; 1.
DR SMART; SM00318; SNc; 1.
DR SUPFAM; SSF50199; SSF50199; 1.
DR PROSITE; PS01123; TNASE_1; 1.
DR PROSITE; PS01284; TNASE_2; 1.
DR PROSITE; PS50830; TNASE_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Direct protein sequencing; Endonuclease; Hydrolase;
KW Membrane; Metal-binding; Nuclease; Secreted; Signal; Zymogen.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT PROPEP 27..63
FT /evidence="ECO:0000269|PubMed:893427"
FT /id="PRO_0000045226"
FT CHAIN 64..231
FT /note="Nuclease B"
FT /id="PRO_0000034389"
FT PROPEP 64..82
FT /evidence="ECO:0000269|PubMed:4324890"
FT /id="PRO_0000034390"
FT CHAIN 83..231
FT /note="Nuclease A"
FT /id="PRO_0000034391"
FT REGION 61..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 203..223
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 117
FT /evidence="ECO:0000269|PubMed:288045"
FT ACT_SITE 125
FT /evidence="ECO:0000269|PubMed:288045"
FT ACT_SITE 169
FT /evidence="ECO:0000269|PubMed:288045"
FT BINDING 103
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 122
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 123
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT CONFLICT 30
FT /note="V -> M (in Ref. 3; AAA26659)"
FT /evidence="ECO:0000305"
FT TURN 84..86
FT /evidence="ECO:0007829|PDB:2SNS"
FT STRAND 91..99
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 101..109
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 112..118
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 126..128
FT /evidence="ECO:0007829|PDB:4H7B"
FT STRAND 129..131
FT /evidence="ECO:0007829|PDB:2SNS"
FT STRAND 134..136
FT /evidence="ECO:0007829|PDB:1JOK"
FT HELIX 137..150
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 166..168
FT /evidence="ECO:0007829|PDB:1NSN"
FT STRAND 170..176
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 177..179
FT /evidence="ECO:0007829|PDB:2SOB"
FT HELIX 181..187
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 190..193
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:2SNS"
FT TURN 201..203
FT /evidence="ECO:0007829|PDB:1ENA"
FT HELIX 204..216
FT /evidence="ECO:0007829|PDB:4EQP"
FT HELIX 220..222
FT /evidence="ECO:0007829|PDB:4EQP"
FT STRAND 227..229
FT /evidence="ECO:0007829|PDB:1JOK"
SQ SEQUENCE 231 AA; 25471 MW; AD5558AA28E855D7 CRC64;
MLVMTEYLLS AGICMAIVSI LLIGMAISNV SKGQYAKRFF FFATSCLVLT LVVVSSLSSS
ANASQTDNGV NRSGSEDPTV YSATSTKKLH KEPATLIKAI DGDTVKLMYK GQPMTFRLLL
VDTPETKHPK KGVEKYGPEA SAFTKKMVEN AKKIEVEFDK GQRTDKYGRG LAYIYADGKM
VNEALVRQGL AKVAYVYKPN NTHEQHLRKS EAQAKKEKLN IWSEDNADSG Q
