ID   NUDJ_ECOK1              Reviewed;         153 AA.
AC   A1AA28;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Phosphatase NudJ;
DE            EC=3.6.1.-;
GN   Name=nudJ; OrderedLocusNames=Ecok1_10240; ORFNames=APECO1_216;
OS   Escherichia coli O1:K1 / APEC.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=405955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17293413; DOI=10.1128/jb.01726-06;
RA   Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J.,
RA   Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
RT   "The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7
RT   shares strong similarities with human extraintestinal pathogenic E. coli
RT   genomes.";
RL   J. Bacteriol. 189:3228-3236(2007).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Nudix hydrolase family. NudJ subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000468; ABJ00518.1; -; Genomic_DNA.
DR   RefSeq; WP_000476093.1; NC_008563.1.
DR   PDB; 3DKU; X-ray; 2.69 A; A/B/C/D/E/F/G/H=1-153.
DR   PDB; 3SHD; X-ray; 2.50 A; A/B/C/D/E/F/G/H/I/J/K/L=1-153.
DR   PDBsum; 3DKU; -.
DR   PDBsum; 3SHD; -.
DR   AlphaFoldDB; A1AA28; -.
DR   SMR; A1AA28; -.
DR   EnsemblBacteria; ABJ00518; ABJ00518; APECO1_216.
DR   GeneID; 66670599; -.
DR   KEGG; ecv:APECO1_216; -.
DR   HOGENOM; CLU_037162_6_1_6; -.
DR   OMA; QPAGHLD; -.
DR   EvolutionaryTrace; A1AA28; -.
DR   Proteomes; UP000008216; Chromosome.
DR   GO; GO:0017110; F:nucleoside-diphosphatase activity; IEA:InterPro.
DR   GO; GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
DR   GO; GO:0004787; F:thiamine-diphosphatase activity; IEA:InterPro.
DR   CDD; cd03675; Nudix_Hydrolase_2; 1.
DR   InterPro; IPR020476; Nudix_hydrolase.
DR   InterPro; IPR015797; NUDIX_hydrolase-like_dom_sf.
DR   InterPro; IPR020084; NUDIX_hydrolase_CS.
DR   InterPro; IPR000086; NUDIX_hydrolase_dom.
DR   InterPro; IPR033713; NudJ.
DR   Pfam; PF00293; NUDIX; 1.
DR   PRINTS; PR00502; NUDIXFAMILY.
DR   SUPFAM; SSF55811; SSF55811; 1.
DR   PROSITE; PS51462; NUDIX; 1.
DR   PROSITE; PS00893; NUDIX_BOX; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Magnesium.
FT   CHAIN           1..153
FT                   /note="Phosphatase NudJ"
FT                   /id="PRO_0000342642"
FT   DOMAIN          3..131
FT                   /note="Nudix hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00794"
FT   MOTIF           36..57
FT                   /note="Nudix box"
FT   STRAND          5..14
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          17..25
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          28..32
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          34..37
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   HELIX           44..56
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          64..71
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          79..87
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          103..107
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   HELIX           109..113
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          118..120
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   HELIX           122..131
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   HELIX           138..141
FT                   /evidence="ECO:0007829|PDB:3SHD"
FT   STRAND          143..145
FT                   /evidence="ECO:0007829|PDB:3SHD"
SQ   SEQUENCE   153 AA;  17433 MW;  BDB7423E97D6735A CRC64;
     MFKPHVTVAC VVHAEGKFLV VEETINGKAL WNQPAGHLEA DETLVEAAAR ELWEETGISA
     QPQHFIRMHQ WIAPDKTPFL RFLFAIELEQ ICPTQPHDSD IDCCRWVSAE EILQASNLRS
     PLVAESIRCY QSGQRYPLEM IGDFNWPFTK GVI