ID   NUF2A_XENLA             Reviewed;         462 AA.
AC   Q8AWF4;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Kinetochore protein Nuf2-A;
DE            Short=xNuf2;
DE   AltName: Full=Cell division cycle-associated protein 1-A;
GN   Name=nuf2-a; Synonyms=cdca1-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NDC80, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=12514103; DOI=10.1101/gad.1040903;
RA   McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J.,
RA   Burke D.J., Stukenberg P.T.;
RT   "The highly conserved Ndc80 complex is required for kinetochore assembly,
RT   chromosome congression, and spindle checkpoint activity.";
RL   Genes Dev. 17:101-114(2003).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE NDC80
RP   COMPLEX.
RX   PubMed=14738735; DOI=10.1016/j.cub.2003.12.058;
RA   McCleland M.L., Kallio M.J., Barrett-Wilt G.A., Kestner C.A.,
RA   Shabanowitz J., Hunt D.F., Gorbsky G.J., Stukenberg P.T.;
RT   "The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are
RT   required to establish and maintain kinetochore-microtubule attachment.";
RL   Curr. Biol. 14:131-137(2004).
RN   [3]
RP   CHARACTERIZATION OF THE NDC80 COMPLEX, INTERACTION OF THE NDC80 COMPLEX
RP   WITH MIS12 AND ZWINT, AND SUBCELLULAR LOCATION.
RX   PubMed=16079178; DOI=10.1091/mbc.e05-03-0239;
RA   Emanuele M.J., McCleland M.L., Satinover D.L., Stukenberg P.T.;
RT   "Measuring the stoichiometry and physical interactions between components
RT   elucidates the architecture of the vertebrate kinetochore.";
RL   Mol. Biol. Cell 16:4882-4892(2005).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity (PubMed:12514103). Required for kinetochore
CC       integrity and the organization of stable microtubule binding sites in
CC       the outer plate of the kinetochore (By similarity). The NDC80 complex
CC       synergistically enhances the affinity of the SKA1 complex for
CC       microtubules and may allow the NDC80 complex to track depolymerizing
CC       microtubules (By similarity). {ECO:0000250|UniProtKB:Q9BZD4,
CC       ECO:0000269|PubMed:12514103}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC       zwint. {ECO:0000269|PubMed:12514103, ECO:0000269|PubMed:14738735,
CC       ECO:0000269|PubMed:16079178}.
CC   -!- INTERACTION:
CC       Q8AWF4; Q8AWF5: ndc80; NbExp=8; IntAct=EBI-1000342, EBI-1000337;
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC       Note=Localizes to kinetochores from late prophase to anaphase.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR   EMBL; AY168014; AAN87032.1; -; mRNA.
DR   RefSeq; NP_001082370.1; NM_001088901.1.
DR   RefSeq; XP_018112035.1; XM_018256546.1.
DR   AlphaFoldDB; Q8AWF4; -.
DR   SMR; Q8AWF4; -.
DR   IntAct; Q8AWF4; 5.
DR   GeneID; 398428; -.
DR   KEGG; xla:398428; -.
DR   CTD; 398428; -.
DR   Xenbase; XB-GENE-1001444; nuf2.L.
DR   OMA; QCDTERQ; -.
DR   OrthoDB; 752521at2759; -.
DR   Proteomes; UP000186698; Chromosome 4L.
DR   Bgee; 398428; Expressed in testis and 18 other tissues.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF03800; Nuf2; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..462
FT                   /note="Kinetochore protein Nuf2-A"
FT                   /id="PRO_0000249818"
FT   REGION          239..259
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          143..277
FT                   /evidence="ECO:0000255"
FT   COILED          308..461
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   462 AA;  54453 MW;  4C2E5299D9ED6CB0 CRC64;
     MDKLTFPIFP AADLVNFFRQ NILTGTEAKN FNKNDIFPNP KPEMVQKLYM RILQQVFNYG
     VEQFYMVPMD LDIQYPHLVE GFAPVANILK LMARFLPMCR VYDFHPSDVL NPKGKRTLHS
     LSGIVNFLHF SATRKEVYFE YCSSYKSALE NVRQLQKANQ EAEIKIEKLT TVPPEQQAEF
     KALSSEIHDL QQIISQEYRA KDVAFQEKIA QRKTEFAEKN KRLNEQKLAI ATMKEEQERM
     KSQIVESPEQ RKSKTERMKE TVHRLKQARQ ETNDKCDYYR DRVAFACMWQ TDVQGYLKKL
     QGIDTNLEIH RKIREEIRHS EEQVVNLNLE LKSLSNEDAQ LKRIILVKKE KLAKVDIKNK
     KKQEDFNQQK QEILEVCSRI QEKRQVVHGR VAQVLQEIQQ TIGKKEQLLE TTEAGKKKCQ
     EVITDFRAAL EKYHDSLQKA SERSADRRRE KIAELNRRLS RR