NUF2B_XENLA
ID NUF2B_XENLA Reviewed; 462 AA.
AC Q6GQ71; Q498I2;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2006, sequence version 2.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=Kinetochore protein Nuf2-B;
DE AltName: Full=Cell division cycle-associated protein 1-B;
GN Name=nuf2-b; Synonyms=cdca1-b;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity. Required for kinetochore integrity and the
CC organization of stable microtubule binding sites in the outer plate of
CC the kinetochore. The NDC80 complex synergistically enhances the
CC affinity of the SKA1 complex for microtubules and may allow the NDC80
CC complex to track depolymerizing microtubules.
CC {ECO:0000250|UniProtKB:Q9BZD4}.
CC -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC cdca1, spbc24 and spbc25. The NDC80 complex interacts with mis12 and
CC zwint (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC kinetochore {ECO:0000250}. Note=Localizes to kinetochores from late
CC prophase to anaphase. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH72877.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305};
CC Sequence=AAI00208.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BC072877; AAH72877.1; ALT_SEQ; mRNA.
DR EMBL; BC100207; AAI00208.1; ALT_INIT; mRNA.
DR RefSeq; XP_018116557.1; XM_018261068.1.
DR AlphaFoldDB; Q6GQ71; -.
DR SMR; Q6GQ71; -.
DR GeneID; 443588; -.
DR KEGG; xla:443588; -.
DR CTD; 443588; -.
DR Xenbase; XB-GENE-6252105; nuf2.S.
DR OrthoDB; 752521at2759; -.
DR Proteomes; UP000186698; Chromosome 4S.
DR Bgee; 443588; Expressed in blastula and 11 other tissues.
DR GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR Gene3D; 1.10.418.60; -; 1.
DR InterPro; IPR005549; Kinetochore_Nuf2_N.
DR InterPro; IPR038275; Nuf2_N_sf.
DR Pfam; PF03800; Nuf2; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW Kinetochore; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..462
FT /note="Kinetochore protein Nuf2-B"
FT /id="PRO_0000249819"
FT REGION 236..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 143..462
FT /evidence="ECO:0000255"
FT CONFLICT 390
FT /note="R -> H (in Ref. 1; AAH72877)"
FT /evidence="ECO:0000305"
FT CONFLICT 423
FT /note="I -> V (in Ref. 1; AAH72877)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 462 AA; 54631 MW; 0A4D7726AC21B7F5 CRC64;
MDKLTFPIFP AADLVNFFRQ NILTGTEAKN FNKNDLYPNP KPEMVQKLYM RILQQVFSYG
VEQFYMVPMD LDIQYPHLVE GFAPVANILK LMARLLPMCR VYDFHPSDVL NPKGKRTLHL
LSGIFNFLQF RTTQREVYME YCSGYKSALE NVRQLQKTNH EAEIKIEKLT TVPPEQQAEF
KALSSEIHDL QQIISQEYRA KDVMFQEKIA QRKAEFAEKN KRLNEQKLTI ATMKEEQERM
KSQIVESPEQ RKSKTERMKE TVHRLKQARQ ETSDKCDHYR DRVALAFMWQ SDVQGYLKKL
QNIDANLEIH RKIHEEIRHI EEQLMNLNLE LKSLSNEDAQ LKRIILVKKE KLAKVDIKNK
KKQEDFNQQK QEILEVCSHI QEKRQVIHGR VAQVLQEIQQ TISKKEQLLE TTEAGKNKCQ
EVITDFRAAL EKYHDSLQKA SERSADRRRE KIAELNRRLS RQ