NUF2_ARATH
ID NUF2_ARATH Reviewed; 440 AA.
AC Q8RXJ0; Q9C953;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 122.
DE RecName: Full=Kinetochore protein NUF2 homolog {ECO:0000303|PubMed:29356153};
GN Name=NUF2 {ECO:0000303|PubMed:29356153};
GN OrderedLocusNames=At1g61000 {ECO:0000312|Araport:AT1G61000};
GN ORFNames=T7P1.14 {ECO:0000312|EMBL:AAG51636.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=29356153; DOI=10.1111/tpj.13823;
RA Shin J., Jeong G., Park J.Y., Kim H., Lee I.;
RT "MUN (MERISTEM UNSTRUCTURED), encoding a SPC24 homolog of NDC80 kinetochore
RT complex, affects development through cell division in Arabidopsis
RT thaliana.";
RL Plant J. 93:977-991(2018).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity to ensure proper cell division.
CC {ECO:0000305|PubMed:29356153}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC SPC24 and SPC25. {ECO:0000269|PubMed:29356153}.
CC -!- SUBCELLULAR LOCATION: Chromosome, centromere
CC {ECO:0000269|PubMed:29356153}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to division arrest before
CC the globular stage. {ECO:0000269|PubMed:29356153}.
CC -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51636.1; Type=Erroneous gene model prediction; Note=The predicted gene has been split into 2 genes: At1g60995 and At1g61000.; Evidence={ECO:0000305};
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DR EMBL; AC018908; AAG51636.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33765.1; -; Genomic_DNA.
DR EMBL; AY080858; AAL87331.1; -; mRNA.
DR EMBL; AY133856; AAM91790.1; -; mRNA.
DR PIR; F96635; F96635.
DR RefSeq; NP_176296.2; NM_104781.3.
DR AlphaFoldDB; Q8RXJ0; -.
DR SMR; Q8RXJ0; -.
DR STRING; 3702.AT1G61000.1; -.
DR iPTMnet; Q8RXJ0; -.
DR PaxDb; Q8RXJ0; -.
DR PRIDE; Q8RXJ0; -.
DR ProteomicsDB; 248749; -.
DR EnsemblPlants; AT1G61000.1; AT1G61000.1; AT1G61000.
DR GeneID; 842392; -.
DR Gramene; AT1G61000.1; AT1G61000.1; AT1G61000.
DR KEGG; ath:AT1G61000; -.
DR Araport; AT1G61000; -.
DR TAIR; locus:2206071; AT1G61000.
DR eggNOG; KOG4438; Eukaryota.
DR HOGENOM; CLU_025461_0_0_1; -.
DR InParanoid; Q8RXJ0; -.
DR OMA; AHIKLYI; -.
DR OrthoDB; 752521at2759; -.
DR PhylomeDB; Q8RXJ0; -.
DR PRO; PR:Q8RXJ0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RXJ0; baseline and differential.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:TAIR.
DR GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0010342; P:endosperm cellularization; IMP:TAIR.
DR GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR GO; GO:0000070; P:mitotic sister chromatid segregation; IMP:TAIR.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:TAIR.
DR Gene3D; 1.10.418.60; -; 1.
DR InterPro; IPR005549; Kinetochore_Nuf2_N.
DR InterPro; IPR038275; Nuf2_N_sf.
DR Pfam; PF03800; Nuf2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Mitosis;
KW Reference proteome.
FT CHAIN 1..440
FT /note="Kinetochore protein NUF2 homolog"
FT /id="PRO_0000444103"
FT COILED 142..239
FT /evidence="ECO:0000255"
FT COILED 299..386
FT /evidence="ECO:0000255"
SQ SEQUENCE 440 AA; 50532 MW; D45735074FDC5743 CRC64;
MSAYEYPRLS RSDIITALKD AQIASVTETD LKTPTSDFVS ELYTRILIYL DALDEEEKGQ
VDFEALEQLE NPDHHATSMQ AMKLYCKVKD MLEMLDCPLP ISFKDLLRPE SSRTEFFISA
LLNYGLYKDS KMDLIRPKAE ELGLLDEQRK QCEAKVAQLN AEIGEFDEAV ERDLPFVQEL
EANIEQLNKK ILELNNQQMS LRATFQKMRE KSTQMDNEIS KAEFDLVETV QENANLRSQI
VQSPDKLQGA LEEKKLVLGE TKKAEQSAMV TFQEKAAILE VFEKALKKIL KSSSQLQLIN
EQVTNAKTVE KEFKALKDKL SEDGVAYKSL EAKVVERERI VEQLNESLKQ LEKEKAVMFD
DWTKQLNELK VEVESRRREL ETRQTNVESV VAMVDDNTAK TNQVRQSGEA KVKKLAAKYE
EIVKQFHEYT VSFDAFLPSL
