ID   NUF2_ASHGO              Reviewed;         455 AA.
AC   Q757M3;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Probable kinetochore protein NUF2;
GN   Name=NUF2; OrderedLocusNames=AEL015W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC       SPC24 and SPC25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Note=Associated with kinetochores.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR   EMBL; AE016818; AAS52670.1; -; Genomic_DNA.
DR   RefSeq; NP_984846.1; NM_210200.1.
DR   AlphaFoldDB; Q757M3; -.
DR   SMR; Q757M3; -.
DR   STRING; 33169.AAS52670; -.
DR   EnsemblFungi; AAS52670; AAS52670; AGOS_AEL015W.
DR   GeneID; 4621045; -.
DR   KEGG; ago:AGOS_AEL015W; -.
DR   eggNOG; KOG4438; Eukaryota.
DR   HOGENOM; CLU_025461_2_0_1; -.
DR   InParanoid; Q757M3; -.
DR   OMA; AHIKLYI; -.
DR   Proteomes; UP000000591; Chromosome V.
DR   GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005876; C:spindle microtubule; IEA:EnsemblFungi.
DR   GO; GO:0005816; C:spindle pole body; IEA:EnsemblFungi.
DR   GO; GO:0008017; F:microtubule binding; IEA:EnsemblFungi.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR   GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF03800; Nuf2; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..455
FT                   /note="Probable kinetochore protein NUF2"
FT                   /id="PRO_0000246644"
FT   COILED          184..298
FT                   /evidence="ECO:0000255"
FT   COILED          343..380
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   455 AA;  53091 MW;  2182FDDCA9C212C2 CRC64;
     MSSDNFPLLD IPELVTCLQE CDFSLATIQN VERPSSQFVI TLYKQIIDTF MGVSPDSLLE
     DSRNFVENGD TSEQEVYDAS LVYHDTLKIL ALNKICYKFF LDIGVSDFNV MDLYKPDPHR
     TRRFLSAVAN YARFREEAML ELDGDQHPDG VRYLEQTDKL LKELRLKFDA NNLLHSRVQK
     YKEFQKSDDL EALESDNKNM ESELRHLNQV QETLTVDYER YKAEKQRHLS ELETLGYELI
     ELESERDKLR KYSQTDAEVL RKGVRELSLL LEQQQVQLEK LEQKQKNLQI SFETFQASTQ
     ELYEVLQIVS TDLQDSHLKE STLLDAKHKL MQNHKNLNNL LSAGIMAKIT LLQEQLDSQK
     RKLAELELNT QAKESENSAT LKRLRKQHTD DVLPEVRNIE QRYKTKVADT ITNYESQMRN
     IKVDFDNEVA LIENECSLLF NHIKNYMTTI GEKLG