NUF2_CHICK
ID NUF2_CHICK Reviewed; 469 AA.
AC Q76I90; Q5ZI77;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Kinetochore protein Nuf2;
DE AltName: Full=Cell division cycle-associated protein 1;
GN Name=NUF2; Synonyms=CDCA1; ORFNames=RCJMB04_29i8;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NDC80, AND
RP SUBCELLULAR LOCATION.
RX PubMed=12829748; DOI=10.1242/jcs.00645;
RA Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T.;
RT "Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and
RT centromere and is essential for mitotic progression in vertebrate cells.";
RL J. Cell Sci. 116:3347-3362(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
RN [3]
RP INTERACTION WITH CENPH.
RX PubMed=15713649; DOI=10.1128/mcb.25.5.1958-1970.2005;
RA Mikami Y., Hori T., Kimura H., Fukagawa T.;
RT "The functional region of CENP-H interacts with the Nuf2 complex that
RT localizes to centromere during mitosis.";
RL Mol. Cell. Biol. 25:1958-1970(2005).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=15870271; DOI=10.1128/mcb.25.10.3967-3981.2005;
RA Regnier V., Vagnarelli P., Fukagawa T., Zerjal T., Burns E., Trouche D.,
RA Earnshaw W., Brown W.;
RT "CENP-A is required for accurate chromosome segregation and sustained
RT kinetochore association of BubR1.";
RL Mol. Cell. Biol. 25:3967-3981(2005).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity (PubMed:12829748). Required for kinetochore
CC integrity and the organization of stable microtubule binding sites in
CC the outer plate of the kinetochore (By similarity). The NDC80 complex
CC synergistically enhances the affinity of the SKA1 complex for
CC microtubules and may allow the NDC80 complex to track depolymerizing
CC microtubules (By similarity). {ECO:0000250|UniProtKB:Q9BZD4,
CC ECO:0000269|PubMed:12829748}.
CC -!- SUBUNIT: Component of the NDC80 complex, which is composed of at least
CC NDC80/HEC1 and CDCA1.
CC -!- INTERACTION:
CC Q76I90; Q76I89: NDC80; NbExp=3; IntAct=EBI-1003866, EBI-1003779;
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, centromere,
CC kinetochore. Cytoplasm, cytoskeleton, microtubule organizing center,
CC centrosome. Note=Localizes to kinetochores from late prophase to
CC anaphase and to the centrosome during G1 and S phase.
CC -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR EMBL; AB098621; BAC81641.1; -; mRNA.
DR EMBL; AJ720907; CAG32566.1; -; mRNA.
DR RefSeq; NP_989809.2; NM_204478.3.
DR AlphaFoldDB; Q76I90; -.
DR SMR; Q76I90; -.
DR BioGRID; 675432; 2.
DR IntAct; Q76I90; 3.
DR STRING; 9031.ENSGALP00000038992; -.
DR PaxDb; Q76I90; -.
DR GeneID; 395135; -.
DR KEGG; gga:395135; -.
DR CTD; 83540; -.
DR VEuPathDB; HostDB:geneid_395135; -.
DR eggNOG; KOG4438; Eukaryota.
DR HOGENOM; CLU_589957_0_0_1; -.
DR InParanoid; Q76I90; -.
DR OrthoDB; 752521at2759; -.
DR PhylomeDB; Q76I90; -.
DR TreeFam; TF101067; -.
DR PRO; PR:Q76I90; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR Gene3D; 1.10.418.60; -; 1.
DR InterPro; IPR005549; Kinetochore_Nuf2_N.
DR InterPro; IPR038275; Nuf2_N_sf.
DR Pfam; PF03800; Nuf2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..469
FT /note="Kinetochore protein Nuf2"
FT /id="PRO_0000249816"
FT COILED 135..444
FT /evidence="ECO:0000255"
FT CONFLICT 411
FT /note="S -> N (in Ref. 2; CAG32566)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 469 AA; 55127 MW; 5327A06E09028104 CRC64;
MEALTFPRYS PDDIVAYLRS HVLVGAEARN LTKADLFATL KPEVLHMIFI RILQKVYGIR
LEHFYMMPVN VDIVYPQIFE GFLPVCNLYI HMERFLPVCR VNDFQMSDVI NPKAKRTARF
LSGILNFVHF RECRREAYLE LQLNYKSAME KHQQLETANQ ELEMKLEKLN TVPVEQQAEF
KQLSDDIQEL EQLLSHDYRR KTAALQEVIS QKKSDITERT RKLNELKVTM ATLKEEQEQL
KSKIVESPEE LKNYKELMKE TVKKLKKSKQ EVIEKYEGYR DLVEVLPSCQ LEVQLYQKKM
ERQAANVERL ASVLSEVRNL EDQLESAQIE LKKGKTDEMS LKRLVTAKHE RLSTAEIRIK
KKREDVEQYK HTVFEYFNRV QEKRGVVYDK VTAIQKEIQQ MRFKIQQLNE SAEEEEMKAK
EIYQNLKAGL EKRHDSLIKT AKNYAASRED KIAELQKGLL RVQSPGSSS
