ID   NUF2_CRYNJ              Reviewed;         467 AA.
AC   P0CP40; Q55Z49; Q5KNG4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Probable kinetochore protein NUF2;
GN   Name=NUF2; OrderedLocusNames=CNA06580;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of at least
CC       NDC80, NUF2 and SPC25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Note=Associated with kinetochores.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW41179.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AE017341; AAW41179.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_566998.1; XM_566998.1.
DR   AlphaFoldDB; P0CP40; -.
DR   SMR; P0CP40; -.
DR   STRING; 5207.AAW41179; -.
DR   PaxDb; P0CP40; -.
DR   eggNOG; KOG4438; Eukaryota.
DR   InParanoid; P0CP40; -.
DR   OrthoDB; 752521at2759; -.
DR   Proteomes; UP000002149; Chromosome 1.
DR   GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR   GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR041112; Nuf2_DHR10-like.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF18595; DHR10; 1.
DR   Pfam; PF03800; Nuf2; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..467
FT                   /note="Probable kinetochore protein NUF2"
FT                   /id="PRO_0000246649"
FT   COILED          159..249
FT                   /evidence="ECO:0000255"
FT   COILED          282..431
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   467 AA;  54295 MW;  3296A7D1BF52DC6A CRC64;
     MSQQNRRVQQ NTAAFPLLTA HDILECLAAL DIPAQMEDLT KPTAQSTQSI YGSLLEVLMG
     ASINSIEGPK QALLGMMEYK VLSFRSMKRT QINLSRQEMY SDTLQFMMFF KHCRRLALLC
     GIPDFAISDL ARPDANRLRK VLSGIMNFAK FRDERMQTQA RFQENLQKHQ KKAVDLRRKT
     EELETQFQEI TARNAAERPQ SEQAQKRNEL LKSELLELNS QRLKEVQEYE ELKKERQTLL
     EQVNHNNRIV TQLELQIGSA KSRLVQSPDR IKRHISEMSF AIQSEKAKLA SFQQKARELT
     NRLEVIGALE VDLRGLIDLE HSIQDQRAKT EEAKRSKSAL EARLEGRQIE SQGLAAKLEQ
     LQRQLQNASH KLARQEETRK GMRERGARRI DELKAEYKVR ARERGEWQKQ RDDLLAEQKE
     LESEMAAFVT KHENEINEFL HAYWTMRRQA EDYMNTMTVK LGLQTAI