ID   NUF2_DANRE              Reviewed;         454 AA.
AC   Q7ZW63;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Kinetochore protein Nuf2;
DE   AltName: Full=Cell division cycle-associated protein 1;
GN   Name=nuf2; Synonyms=cdca1; ORFNames=zgc:56708;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=SJD;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which is composed of ndc80,
CC       cdca1, spbc24 and spbc25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Note=Localizes to kinetochores from late
CC       prophase to anaphase. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR   EMBL; BC050181; AAH50181.1; -; mRNA.
DR   RefSeq; NP_956604.1; NM_200310.1.
DR   RefSeq; XP_005155728.1; XM_005155671.2.
DR   AlphaFoldDB; Q7ZW63; -.
DR   SMR; Q7ZW63; -.
DR   STRING; 7955.ENSDARP00000040811; -.
DR   PaxDb; Q7ZW63; -.
DR   PRIDE; Q7ZW63; -.
DR   Ensembl; ENSDART00000040812; ENSDARP00000040811; ENSDARG00000034624.
DR   GeneID; 393280; -.
DR   KEGG; dre:393280; -.
DR   CTD; 83540; -.
DR   ZFIN; ZDB-GENE-040426-1121; nuf2.
DR   eggNOG; KOG4438; Eukaryota.
DR   GeneTree; ENSGT00390000004199; -.
DR   InParanoid; Q7ZW63; -.
DR   OMA; AHIKLYI; -.
DR   OrthoDB; 752521at2759; -.
DR   PhylomeDB; Q7ZW63; -.
DR   TreeFam; TF101067; -.
DR   Reactome; R-DRE-141444; Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
DR   Reactome; R-DRE-2467813; Separation of Sister Chromatids.
DR   Reactome; R-DRE-2500257; Resolution of Sister Chromatid Cohesion.
DR   Reactome; R-DRE-5663220; RHO GTPases Activate Formins.
DR   Reactome; R-DRE-9648025; EML4 and NUDC in mitotic spindle formation.
DR   PRO; PR:Q7ZW63; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 11.
DR   Bgee; ENSDARG00000034624; Expressed in testis and 28 other tissues.
DR   ExpressionAtlas; Q7ZW63; baseline.
DR   GO; GO:0031262; C:Ndc80 complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007507; P:heart development; IMP:ZFIN.
DR   GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR   GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF03800; Nuf2; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..454
FT                   /note="Kinetochore protein Nuf2"
FT                   /id="PRO_0000249817"
FT   COILED          143..347
FT                   /evidence="ECO:0000255"
FT   COILED          375..439
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   454 AA;  53632 MW;  271A221F42BB7D95 CRC64;
     MSENTFPVYK VDVIVQFYRT EVLTGQESKH FTKNDLTPTP KPESVQRLYM RILQLLFRFR
     PECHYTVPLS ENIQYPMLYE SFAPIMSVYM RMCQFLPVCR VYDFSLSDLL NPKTKRTITI
     LSAIQNFLHF RKQRLEITAA HQQSFRADMD RLQAYTREIK EAEKKIEKLT TIPPEQQAEA
     KELASALAEL STNTQHEYQD VSAINEKVAQ FKTEIAELSQ KLTQRKVEVA TLKDEISKLK
     SQIVESPEEL KNEMERMRET AKNIKMSKEL ADERLVELQM LVQCASQVEA EIQILLKQLQ
     DLQSSMSKTK QRKEEVQSLE VMNESLQKEL KSLSSEEAQL KRALTMKLDK ESKQQIRRQK
     KKEVKDQQVK NIYGQYDKMH QKRQEIVKKI EECNRETKQF KEKMQALREN CNQRTQKAQE
     IYERLLTTLE QYHKRIEKIL VETNADALKM KSHF