NUF2_EMENI
ID NUF2_EMENI Reviewed; 463 AA.
AC Q5BH14; C8VQ44;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 2.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Probable kinetochore protein nuf2;
GN Name=nuf2; ORFNames=AN0166;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity. {ECO:0000250}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of ndc80, nuf2,
CC spc24 and spc25. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC kinetochore {ECO:0000250}. Note=Associated with kinetochores.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CBF90058.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=EAA66039.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AACD01000005; EAA66039.1; ALT_INIT; Genomic_DNA.
DR EMBL; BN001308; CBF90058.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_657770.1; XM_652678.1.
DR AlphaFoldDB; Q5BH14; -.
DR SMR; Q5BH14; -.
DR STRING; 162425.CADANIAP00002571; -.
DR EnsemblFungi; EAA66039; EAA66039; AN0166.2.
DR GeneID; 2875944; -.
DR KEGG; ani:AN0166.2; -.
DR VEuPathDB; FungiDB:AN0166; -.
DR eggNOG; KOG4438; Eukaryota.
DR HOGENOM; CLU_025461_2_1_1; -.
DR InParanoid; Q5BH14; -.
DR OrthoDB; 752521at2759; -.
DR Proteomes; UP000000560; Chromosome VIII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR Gene3D; 1.10.418.60; -; 1.
DR InterPro; IPR005549; Kinetochore_Nuf2_N.
DR InterPro; IPR041112; Nuf2_DHR10-like.
DR InterPro; IPR038275; Nuf2_N_sf.
DR Pfam; PF18595; DHR10; 1.
DR Pfam; PF03800; Nuf2; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW Kinetochore; Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..463
FT /note="Probable kinetochore protein nuf2"
FT /id="PRO_0000246651"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..354
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 166..455
FT /evidence="ECO:0000255"
FT COMPBIAS 1..16
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 463 AA; 54529 MW; 5640B270B1D47424 CRC64;
MAYNHRISQQ FHSSQQHGRG SRKKEDENDA LMRLPDKEIA GCINDIGIPF TAADLIKPNP
QQVQMVLEWF AELLMNTTRE TVEPAMRAAA DDICGDFPDI VPTDTRNLMG FFVNMRRLMA
ECGVNDFTFT DLTKPTHDRL VKIFSYLINF VRFRESQTAV IDEHFNKTEK TKQRIETLYT
ENQEMEQRLE EMRRVLKANE AEVKEKVRRN DELKSRLREL GRTQEKVAET LERVKAEKAR
QQNLLKEKME RTVRTRQEVE KLRPYVMESP ASLQSSLTEL SESLLREKNQ IDAMEKRARA
LQTSSDTFTV VSNDVQACIK LLEDIAVELQ KEEDEESRAS RNKEAISERG NNVREVEQTE
KLLQRQLARW NERIEALRNT AHEKAQVAQK RMEELREVQI KLREERTEKQ RDMERRRIRI
EQTEKKMADL KESIETEIQS AHDEYLKLES HIKLYITEME KSL
