ID   NUF2_KLULA              Reviewed;         451 AA.
AC   Q6CJ06;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Probable kinetochore protein NUF2;
GN   Name=NUF2; OrderedLocusNames=KLLA0F22451g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of NDC80, NUF2,
CC       SPC24 and SPC25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Note=Associated with kinetochores.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98791.1; -; Genomic_DNA.
DR   RefSeq; XP_456083.1; XM_456083.1.
DR   AlphaFoldDB; Q6CJ06; -.
DR   SMR; Q6CJ06; -.
DR   STRING; 28985.XP_456083.1; -.
DR   EnsemblFungi; CAG98791; CAG98791; KLLA0_F22451g.
DR   GeneID; 2895393; -.
DR   KEGG; kla:KLLA0_F22451g; -.
DR   eggNOG; KOG4438; Eukaryota.
DR   HOGENOM; CLU_025461_2_0_1; -.
DR   InParanoid; Q6CJ06; -.
DR   OMA; AHIKLYI; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0031262; C:Ndc80 complex; IEA:EnsemblFungi.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005876; C:spindle microtubule; IEA:EnsemblFungi.
DR   GO; GO:0005816; C:spindle pole body; IEA:EnsemblFungi.
DR   GO; GO:0008017; F:microtubule binding; IEA:EnsemblFungi.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IEA:EnsemblFungi.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF03800; Nuf2; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..451
FT                   /note="Probable kinetochore protein NUF2"
FT                   /id="PRO_0000246654"
FT   COILED          157..368
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   451 AA;  52887 MW;  3E646CB9D2E40B31 CRC64;
     MSKDVFPLLE LSELVTCLQS CDFSLAVEEN ISKPSSQYVI TLYKQIIDTF MGVSPDTLLS
     NEALFDNSGS NDIQQNPAYT ETVKVLALNK ICFKFFQDIG VSDFNMMDLY KPDSLRTRRL
     LSAVVNYARF REERMFDCDK FMSKTEFLLN QLRSKFDDYN YLQQQINKHR NEIELREGET
     FETLQQQNKH LDQQISRLKG LQETLNIDYN AYKSRKQSLL HDLEKFGFEL IELEFERNKL
     EKHSKTDFNE LNLNIEKLST LLATQQTNLN NLEQKHRKLR ISMNTFQTLT HELYDVLQLI
     STDLQESHLR EANLIDMREQ LLQTDQKLNN ILSSGVMVKM KILQSQLDNQ KKKLHQLEGE
     TKAKQIENSE TLQLLQRQYT DEVVPDLRAA EEQVENDLAN GTIKNYDYEI SQLKQAFQTE
     IDAIDLEYSL LSSHINRYME EMLRVMVEVT S