ID   NUF2_NEUCR              Reviewed;         464 AA.
AC   Q7S9H0;
DT   25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Probable kinetochore protein nuf2;
DE   AltName: Full=Kinetochore protein 2;
GN   Name=kpr-2; Synonyms=nuf2; ORFNames=NCU06568;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC       NDC80 complex, which is required for chromosome segregation and spindle
CC       checkpoint activity. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the NDC80 complex, which consists of kpr-1/ndc80,
CC       kpr-2/nuf2, kpr-3/spc24 and kpr-4/spc25. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Note=Associated with kinetochores.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR   EMBL; CM002239; EAA33039.1; -; Genomic_DNA.
DR   RefSeq; XP_962275.1; XM_957182.2.
DR   AlphaFoldDB; Q7S9H0; -.
DR   SMR; Q7S9H0; -.
DR   STRING; 5141.EFNCRP00000006296; -.
DR   PRIDE; Q7S9H0; -.
DR   EnsemblFungi; EAA33039; EAA33039; NCU06568.
DR   GeneID; 3878398; -.
DR   KEGG; ncr:NCU06568; -.
DR   VEuPathDB; FungiDB:NCU06568; -.
DR   HOGENOM; CLU_025461_2_1_1; -.
DR   InParanoid; Q7S9H0; -.
DR   OMA; AHIKLYI; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0031262; C:Ndc80 complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR   GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR   GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR   GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR   Gene3D; 1.10.418.60; -; 1.
DR   InterPro; IPR005549; Kinetochore_Nuf2_N.
DR   InterPro; IPR041112; Nuf2_DHR10-like.
DR   InterPro; IPR038275; Nuf2_N_sf.
DR   Pfam; PF18595; DHR10; 1.
DR   Pfam; PF03800; Nuf2; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Coiled coil;
KW   Kinetochore; Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..464
FT                   /note="Probable kinetochore protein nuf2"
FT                   /id="PRO_0000246655"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          159..454
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   464 AA;  53827 MW;  CBACD19707B34D55 CRC64;
     MAFNPRMSIL PPQPQQSRQR KKEEEAAYAN MRLPDREIVG CINELGIPFT LADLQKPNPI
     QVQMIFEWFG ELLMNKTRQT VDPAMRAAAE DVCGPELGEA MMPSDTRNLL GFYVSLRRLM
     LDCGVNDFSF NDLYKPTHDR LVRMLSYVIN FVRFRESQTS VIDEHCNKAE QTKARIEQLY
     VENQNMEAQL EEMRHNRRAM EVLVQEKTVR NEELKKRLLE LRRSQEKVAA RLEEAKTKKG
     ELAAELEEKT ATKIALKQES AKLRPYVLQS PSALQASLAE LSNTLNNDKA HIDALDRRSR
     ALQTSTDSFS VVASDVASCI KLLEEIAIEL AKEEEENVKN ARQRDALTER GHNVREVERT
     EALLKRQLAK WVDRTAQLRT QSEEKAQKAM EKMEELRAVH RKLTEERSEK GKDIERRRVR
     IEQTEKKMLD LKENIENEVH AAHDEYLKME AHIKLYITEM EQAI