NUF2_SCHPO
ID NUF2_SCHPO Reviewed; 441 AA.
AC Q10173;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Kinetochore protein nuf2;
DE AltName: Full=NMS complex subunit nuf2;
GN Name=nuf2; ORFNames=SPAC27F1.04c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11685532; DOI=10.1007/s004120100153;
RA Nabetani A., Koujin T., Tsutsumi C., Haraguchi T., Hiraoka Y.;
RT "A conserved protein, Nuf2, is implicated in connecting the centromere to
RT the spindle during chromosome segregation: a link between the kinetochore
RT function and the spindle checkpoint.";
RL Chromosoma 110:322-334(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP IDENTIFICATION IN THE NDC80 COMPLEX, IDENTIFICATION IN THE NMS COMPLEX, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
RN [4]
RP FUNCTION OF THE NDC80 COMPLEX, SELF-ASSOCIATION, INTERACTION WITH NDC80,
RP AND SUBCELLULAR LOCATION.
RX PubMed=15728720; DOI=10.1091/mbc.e04-11-0996;
RA Asakawa H., Hayashi A., Haraguchi T., Hiraoka Y.;
RT "Dissociation of the Nuf2-Ndc80 complex releases centromeres from the
RT spindle-pole body during meiotic prophase in fission yeast.";
RL Mol. Biol. Cell 16:2325-2338(2005).
RN [5]
RP IDENTIFICATION IN THE NMS COMPLEX.
RX PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT "Reconstruction of the kinetochore during meiosis in fission yeast
RT Schizosaccharomyces pombe.";
RL Mol. Biol. Cell 17:5173-5184(2006).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143 AND SER-242, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: Acts as a component of the NMS (Ndc80-MIND-Spc7) super
CC complex which has a role in kinetochore function during late meiotic
CC prophase and throughout the mitotic cell cycle. Acts as a component of
CC the essential kinetochore-associated NDC80 complex, which is required
CC for chromosome segregation and spindle checkpoint activity.
CC {ECO:0000269|PubMed:11685532, ECO:0000269|PubMed:15728720}.
CC -!- SUBUNIT: Component of the NDC80 complex, which consists of ndc80, nuf2,
CC spc24 and spc25. Can self-associate. Component of the NMS super complex
CC which consists of mis12, mis13, mis14, ndc80, nnf1, nuf2, sos7, spc7,
CC spc24 and spc25. {ECO:0000269|PubMed:16079914,
CC ECO:0000269|PubMed:17035632}.
CC -!- INTERACTION:
CC Q10173; Q9URY2: alp7; NbExp=3; IntAct=EBI-1002565, EBI-1556697;
CC Q10173; Q10198: ndc80; NbExp=3; IntAct=EBI-1002565, EBI-1002524;
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore.
CC Cytoplasm, cytoskeleton, microtubule organizing center, spindle pole
CC body. Note=Associated with kinetochores.
CC -!- SIMILARITY: Belongs to the NUF2 family. {ECO:0000305}.
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DR EMBL; CU329670; CAA93293.1; -; Genomic_DNA.
DR PIR; T38462; T38462.
DR RefSeq; NP_594532.1; NM_001019961.2.
DR AlphaFoldDB; Q10173; -.
DR SMR; Q10173; -.
DR BioGRID; 278487; 19.
DR ComplexPortal; CPX-549; Ndc80 complex.
DR DIP; DIP-36501N; -.
DR IntAct; Q10173; 7.
DR STRING; 4896.SPAC27F1.04c.1; -.
DR iPTMnet; Q10173; -.
DR MaxQB; Q10173; -.
DR PaxDb; Q10173; -.
DR PRIDE; Q10173; -.
DR EnsemblFungi; SPAC27F1.04c.1; SPAC27F1.04c.1:pep; SPAC27F1.04c.
DR GeneID; 2542004; -.
DR KEGG; spo:SPAC27F1.04c; -.
DR PomBase; SPAC27F1.04c; nuf2.
DR VEuPathDB; FungiDB:SPAC27F1.04c; -.
DR eggNOG; KOG4438; Eukaryota.
DR HOGENOM; CLU_025461_1_1_1; -.
DR InParanoid; Q10173; -.
DR OMA; AHIKLYI; -.
DR PhylomeDB; Q10173; -.
DR PRO; PR:Q10173; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000775; C:chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0035974; C:meiotic spindle pole body; IDA:PomBase.
DR GO; GO:0031262; C:Ndc80 complex; IDA:PomBase.
DR GO; GO:0031617; C:NMS complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; IC:PomBase.
DR GO; GO:0140483; F:kinetochore adaptor activity; IPI:PomBase.
DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IMP:PomBase.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IC:ComplexPortal.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IC:ComplexPortal.
DR GO; GO:0140456; P:initial meiotic spindle pole body separation; IDA:PomBase.
DR GO; GO:0000073; P:initial mitotic spindle pole body separation; IDA:PomBase.
DR GO; GO:0051383; P:kinetochore organization; IBA:GO_Central.
DR GO; GO:1990571; P:meiotic centromere clustering; IMP:PomBase.
DR GO; GO:0045132; P:meiotic chromosome segregation; IBA:GO_Central.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; IC:ComplexPortal.
DR GO; GO:0007052; P:mitotic spindle organization; IBA:GO_Central.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR Gene3D; 1.10.418.60; -; 1.
DR InterPro; IPR005549; Kinetochore_Nuf2_N.
DR InterPro; IPR041112; Nuf2_DHR10-like.
DR InterPro; IPR038275; Nuf2_N_sf.
DR Pfam; PF18595; DHR10; 1.
DR Pfam; PF03800; Nuf2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Meiosis; Mitosis; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..441
FT /note="Kinetochore protein nuf2"
FT /id="PRO_0000057994"
FT COILED 51..80
FT /evidence="ECO:0000255"
FT COILED 128..186
FT /evidence="ECO:0000255"
FT COILED 316..426
FT /evidence="ECO:0000255"
FT MOD_RES 143
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 242
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 441 AA; 51911 MW; D716FE83223BD16E CRC64;
MARKHTFPSL KRAEILECID GLGIPFTAKE LDQPTSKAVI PLYEEFLDLF MGLTRQNLEE
KVNSLQDSVE NFEIIHESLR FTVFYQILSQ FMQNICFHDF TIQDLLKPDR NRLQLILSAV
INFAKLREER LQQFDDDIQK RESLLETYTL LDAQRKDLEE KVLLSQDRKL ESEAIIKQNE
ERNEEMFQSL IEDKRLCSQV RTEYDRIRME ASELKIRYHN VDSLMASTLE EIEKLQSSIV
HSPEKLKGKI ADTSLRIQND RSQQVELDKK SKILHTKLNS LQLIEGDLNA CLKVLEECLV
ELDKLEHATV LLSTNQELCD QIEINKKKLE FRKEQLLKQL SNAQEKLEHE QHSRNQKLEA
AKQRMDNIRE EYKVITQERN KKIQETEKKN AMIEMTEQKI AGMREELESQ ISSITMEFEK
LKSHVELYIA ELLRNLRSSN S
