NUG2_ORYSJ
ID NUG2_ORYSJ Reviewed; 535 AA.
AC Q10LF7; A0A0P0VYB6; A3AHY4;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Nuclear/nucleolar GTPase 2 {ECO:0000303|PubMed:21205822};
DE Short=OsNug2 {ECO:0000303|PubMed:21205822};
GN Name=NUG2 {ECO:0000303|PubMed:21205822};
GN OrderedLocusNames=Os03g0352400 {ECO:0000312|EMBL:BAF12037.1},
GN LOC_Os03g22890 {ECO:0000312|EMBL:ABF95948.1};
GN ORFNames=OsJ_10852 {ECO:0000312|EMBL:EAZ26923.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|Proteomes:UP000059680};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ACTIVITY
RP REGULATION, SUBCELLULAR LOCATION, AND INTERACTION WITH RPL10A.
RX PubMed=21205822; DOI=10.1074/jbc.m110.200816;
RA Im C.H., Hwang S.M., Son Y.S., Heo J.B., Bang W.Y., Suwastika I.N.,
RA Shiina T., Bahk J.D.;
RT "Nuclear/nucleolar GTPase 2 proteins as a subfamily of YlqF/YawG GTPases
RT function in pre-60S ribosomal subunit maturation of mono- and
RT dicotyledonous plants.";
RL J. Biol. Chem. 286:8620-8632(2011).
CC -!- FUNCTION: GTPase involved in pre-60S ribosomal subunit maturation.
CC {ECO:0000269|PubMed:21205822}.
CC -!- ACTIVITY REGULATION: The GTPase activity is stimulated in the presence
CC of ribosomes, particularly of the 60S subunit.
CC {ECO:0000269|PubMed:21205822}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC Note=kcat is 0.12 min(-1) for GTP. {ECO:0000269|PubMed:21205822};
CC -!- SUBUNIT: Interacts (via N-terminus) with the 60S ribosomal proteins
CC RPL10A. This interaction is enhanced by the addition of GTP.
CC {ECO:0000269|PubMed:21205822}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:21205822}.
CC Nucleus {ECO:0000269|PubMed:21205822}. Note=The N-terminal region (1-
CC 231) is necessary for targeting to the nucleus.
CC {ECO:0000269|PubMed:21205822}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, shoot apical meristem, leaves,
CC leaf sheaths and flowers. {ECO:0000269|PubMed:21205822}.
CC -!- SIMILARITY: Belongs to the TRAFAC class YlqF/YawG GTPase family. RsgA
CC subfamily. {ECO:0000255|PROSITE-ProRule:PRU01058}.
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DR EMBL; DP000009; ABF95948.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF95949.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12037.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS84207.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ26923.1; -; Genomic_DNA.
DR EMBL; AK103394; BAG96058.1; -; mRNA.
DR EMBL; AK105418; BAG97236.1; -; mRNA.
DR EMBL; AK119231; BAG99592.1; -; mRNA.
DR RefSeq; XP_015632277.1; XM_015776791.1.
DR AlphaFoldDB; Q10LF7; -.
DR SMR; Q10LF7; -.
DR STRING; 4530.OS03T0352400-01; -.
DR PaxDb; Q10LF7; -.
DR PRIDE; Q10LF7; -.
DR EnsemblPlants; Os03t0352400-01; Os03t0352400-01; Os03g0352400.
DR GeneID; 4332852; -.
DR Gramene; Os03t0352400-01; Os03t0352400-01; Os03g0352400.
DR KEGG; osa:4332852; -.
DR eggNOG; KOG2423; Eukaryota.
DR HOGENOM; CLU_011106_4_2_1; -.
DR InParanoid; Q10LF7; -.
DR OMA; PAPFQGR; -.
DR OrthoDB; 1210675at2759; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10LF7; OS.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1580.10; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR030378; G_CP_dom.
DR InterPro; IPR006073; GTP-bd.
DR InterPro; IPR023179; GTP-bd_ortho_bundle_sf.
DR InterPro; IPR024929; NOG2.
DR InterPro; IPR012971; NOG2_N_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11089:SF9; PTHR11089:SF9; 1.
DR Pfam; PF01926; MMR_HSR1; 1.
DR Pfam; PF08153; NGP1NT; 1.
DR PRINTS; PR00326; GTP1OBG.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51721; G_CP; 1.
PE 1: Evidence at protein level;
KW GTP-binding; Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..535
FT /note="Nuclear/nucleolar GTPase 2"
FT /id="PRO_0000432557"
FT DOMAIN 213..374
FT /note="CP-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..264
FT /note="G4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 290..292
FT /note="G5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 323..330
FT /note="G1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 349..353
FT /note="G2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 367..370
FT /note="G3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01058"
FT REGION 464..494
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..24
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..494
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 292..293
FT /note="NS -> YR (in Ref. 5; EAZ26923)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 535 AA; 60181 MW; 846C410770E2A8B8 CRC64;
MAKKKERAVN VSGKPRHSLD VNRANDKKGA GGGAGGGGGG RSAATVRRLK MYKMRPLRDR
GGKILKHDLQ SKELPNTRIE PDRRWFGNTR VVNQKELEFF REELQSRLSN NYNVILKERK
LPLSLLQDHQ KQARAHLLDT EPFEHAFGPK GKRKRPKLMA LDYESLLKKA DDSQGAFEDK
HATAKLLKEE EEDGLRDLVR HTMFEKGQSK RIWGELYKVI DSSDVVVQVL DARDPMGTRC
YHLEKHLKEN AKHKHLVFLL NKCDLVPAWA TKGWLRTLSK DYPTLAFHAS INSSFGKGSL
LSVLRQFARL KSDKQAISVG FVGYPNVGKS SVINTLRSKS VCKVAPIPGE TKVWQYITLT
KRIFLIDCPG VVYQNNDSET DIVLKGVVRV TNLADASEHI GEVLRRVKKE HLKRAYKIED
WVDDNDFLVQ LSKTTGKLLR GGEPDLTTTA KMVLHDWQRG KIPFFVPPPQ QGEDSPSETA
EPVEKSDEEG VSSDRTAAAM KAIAGIISSQ QQMNVPCQKE FGVTNEDSEV AEQSE
