ID   NUJM_NEUCR              Reviewed;         200 AA.
AC   P25710; Q7S588;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=NADH-ubiquinone oxidoreductase 21.3 kDa subunit;
DE            EC=7.1.1.2;
GN   ORFNames=B13D24.010, NCU02280;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=74-ORS-6a / FGSC 4200;
RX   PubMed=1825789; DOI=10.1016/0167-4781(91)90074-v;
RA   Nehls U., Hemmer S., Roehlen D.-A., van der Pas J.C., Preis D.,
RA   Sackmann U., Weiss H.;
RT   "cDNA and genomic DNA sequence of the 21.3 kDa subunit of NADH:ubiquinone
RT   reductase (complex I) from Neurospora crassa.";
RL   Biochim. Biophys. Acta 1088:325-326(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Transfer of electrons from NADH to the respiratory chain. The
CC       immediate electron acceptor for the enzyme is believed to be
CC       ubiquinone.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBUNIT: Complex I is composed of about 40 different subunits.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC       Multi-pass membrane protein {ECO:0000305}.
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DR   EMBL; X56612; CAA39949.1; -; Genomic_DNA.
DR   EMBL; BX908789; CAF05866.1; -; Genomic_DNA.
DR   EMBL; CM002242; EAA30688.1; -; Genomic_DNA.
DR   PIR; S14277; S14277.
DR   RefSeq; XP_959924.1; XM_954831.2.
DR   AlphaFoldDB; P25710; -.
DR   SMR; P25710; -.
DR   STRING; 5141.EFNCRP00000003209; -.
DR   TCDB; 3.D.1.6.2; the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.
DR   PRIDE; P25710; -.
DR   EnsemblFungi; EAA30688; EAA30688; NCU02280.
DR   GeneID; 3876046; -.
DR   KEGG; ncr:NCU02280; -.
DR   VEuPathDB; FungiDB:NCU02280; -.
DR   HOGENOM; CLU_088319_0_0_1; -.
DR   InParanoid; P25710; -.
DR   OMA; WGVFTRT; -.
DR   Proteomes; UP000001805; Chromosome 7, Linkage Group VII.
DR   GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
PE   4: Predicted;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; NAD; Oxidoreductase;
KW   Reference proteome; Translocase; Transmembrane; Transmembrane helix;
KW   Ubiquinone.
FT   CHAIN           1..200
FT                   /note="NADH-ubiquinone oxidoreductase 21.3 kDa subunit"
FT                   /id="PRO_0000118738"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        48..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        105..125
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   200 AA;  21349 MW;  B74C1F11CDFAA3DF CRC64;
     MAPQGDDTVF QPKDAIKSGV SGALFSGGAG LLMASLRTSM KKNNVGSMHV FTHGGGTIIS
     FTLAGGIYRF AQQASANLRE KEDGWNHAIG AFLGGSVMGL RSLRFPVILG FGAMAGSVVG
     AFAFSGGLTG WGRDPNVDEF ERKEAMRLNR RRPVEETLAE VGEGRGIYPP GYQERRRQRL
     LEKYGVEVKP VSADPNVASA