A1_BPQBE
ID A1_BPQBE Reviewed; 329 AA.
AC Q8LTE1; D0U1F2; D0U1F6; D0U1G0; D0U1G4; G4WZQ8; G4WZR2; G4WZR6;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2017, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Minor capsid protein A1;
DE AltName: Full=A1 read-through protein {ECO:0000312|EMBL:AAM33127.1};
OS Escherichia virus Qbeta (Bacteriophage Q-beta).
OC Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes;
OC Levivirales; Leviviridae; Allolevivirus.
OX NCBI_TaxID=39803 {ECO:0000312|EMBL:AAM33127.1};
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=7506687; DOI=10.1016/0378-1119(93)90261-z;
RA Kozlovska T.M., Cielens I., Dreilinna D., Dislers A., Baumanis V., Ose V.,
RA Pumpens P.;
RT "Recombinant RNA phage Q-beta capsid particles synthesized and self-
RT assembled in Escherichia coli.";
RL Gene 137:133-137(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=14667253; DOI=10.1186/1471-2148-3-24;
RA Bacher J.M., Bull J.J., Ellington A.D.;
RT "Evolution of phage with chemically ambiguous proteomes.";
RL BMC Evol. Biol. 3:24-24(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=QB_1 {ECO:0000312|EMBL:ACY07223.1},
RC QB_2 {ECO:0000312|EMBL:ACY07227.1}, QB_3 {ECO:0000312|EMBL:ACY07231.1}, and
RC QB_ancestral {ECO:0000312|EMBL:ACY07235.1};
RX PubMed=19956760; DOI=10.1371/journal.pgen.1000742;
RA Domingo-Calap P., Cuevas J.M., Sanjuan R.;
RT "The fitness effects of random mutations in single-stranded DNA and RNA
RT bacteriophages.";
RL PLoS Genet. 5:E1000742-E1000742(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Qbeta_1_FR {ECO:0000312|EMBL:AEQ25541.1},
RC Qbeta_2_FR {ECO:0000312|EMBL:AEQ25545.1}, and
RC Qbeta_3_FR {ECO:0000312|EMBL:AEQ25549.1};
RX PubMed=21967437; DOI=10.1111/j.1558-5646.2011.01339.x;
RA Domingo-Calap P., Sanjuan R.;
RT "Experimental evolution of RNA versus DNA viruses.";
RL Evolution 65:2987-2994(2011).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Anc {ECO:0000312|EMBL:BAP18763.1}, and TW18;
RX PubMed=25056887; DOI=10.1128/jvi.01127-14;
RA Kashiwagi A., Sugawara R., Sano-Tsushima F., Kumagai T., Yomo T.;
RT "Contribution of silent mutations to thermal adaptation of RNA
RT bacteriophage Qbeta.";
RL J. Virol. 88:11459-11468(2014).
RN [6]
RP READTHROUGH.
RX PubMed=5288807; DOI=10.1038/newbio234206a0;
RA Weiner A.M., Weber K.;
RT "Natural read-through at the UGA termination signal of Q-beta coat protein
RT cistron.";
RL Nature New Biol. 234:206-209(1971).
RN [7] {ECO:0007744|PDB:3RLC, ECO:0007744|PDB:3RLK}
RP X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 145-329, FUNCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=21805520; DOI=10.1002/pro.704;
RA Rumnieks J., Tars K.;
RT "Crystal structure of the read-through domain from bacteriophage Qbeta A1
RT protein.";
RL Protein Sci. 20:1707-1712(2011).
CC -!- FUNCTION: Minor capsid protein. {ECO:0000269|PubMed:21805520}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000303|PubMed:21805520}.
CC Note=Present in 3-10 copies per virion. The readthrough extensions are
CC probably located on the exterior of the capsid.
CC {ECO:0000303|PubMed:21805520}.
CC -!- MISCELLANEOUS: This protein is translated as a fusion protein by
CC episodic readthrough of the termination codon at the end of the capsid
CC protein. Readthrough of the terminator codon occurs between the codons
CC for Tyr-133 and Trp-134, thereby producing the readthrough extension.
CC {ECO:0000269|PubMed:5288807}.
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DR EMBL; M99039; AAA16663.1; -; Unassigned_DNA.
DR EMBL; AY099114; AAM33127.1; -; Genomic_RNA.
DR EMBL; GQ153928; ACY07223.1; -; Genomic_RNA.
DR EMBL; GQ153929; ACY07227.1; -; Genomic_RNA.
DR EMBL; GQ153930; ACY07231.1; -; Genomic_RNA.
DR EMBL; GQ153931; ACY07235.1; -; Genomic_RNA.
DR EMBL; JF719735; AEQ25541.1; -; Genomic_RNA.
DR EMBL; JF719736; AEQ25545.1; -; Genomic_RNA.
DR EMBL; JF719737; AEQ25549.1; -; Genomic_RNA.
DR EMBL; AB971354; BAP18763.1; -; Genomic_RNA.
DR PDB; 3RLC; X-ray; 2.90 A; A=145-329.
DR PDB; 3RLK; X-ray; 1.76 A; A=145-329.
DR PDBsum; 3RLC; -.
DR PDBsum; 3RLK; -.
DR SMR; Q8LTE1; -.
DR Proteomes; UP000185268; Genome.
DR Proteomes; UP000305125; Genome.
DR Proteomes; UP000306921; Genome.
DR Proteomes; UP000309733; Genome.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR Gene3D; 3.30.380.10; -; 1.
DR InterPro; IPR002703; Levivir_coat.
DR InterPro; IPR015954; Phage_RNA-type_capsid.
DR InterPro; IPR031819; Read-through_dom.
DR Pfam; PF01819; Levi_coat; 1.
DR Pfam; PF16814; Read-through; 1.
DR SUPFAM; SSF55405; SSF55405; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Capsid protein; Reference proteome; Virion.
FT CHAIN 1..329
FT /note="Minor capsid protein A1"
FT /id="PRO_0000438882"
FT VARIANT 34
FT /note="A -> S (in strain: Qbeta_3_FR)"
FT /evidence="ECO:0000269|PubMed:21967437"
FT VARIANT 76
FT /note="T -> A (in strain: QB_1 and Qbeta_1_FR)"
FT /evidence="ECO:0000269|PubMed:19956760,
FT ECO:0000269|PubMed:21967437"
FT VARIANT 182
FT /note="T -> A (in strain: QB_3, Qbeta_3_FR)"
FT /evidence="ECO:0000269|PubMed:19956760,
FT ECO:0000269|PubMed:21967437"
FT VARIANT 191
FT /note="N -> S (in strain: QB_3, Qbeta_3_FR)"
FT /evidence="ECO:0000269|PubMed:19956760,
FT ECO:0000269|PubMed:21967437"
FT VARIANT 207
FT /note="L -> R (in strain: Qbeta_2_FR)"
FT /evidence="ECO:0000269|PubMed:21967437"
FT VARIANT 211
FT /note="T -> A (in strain: Qbeta_3_FR)"
FT /evidence="ECO:0000269|PubMed:19956760,
FT ECO:0000269|PubMed:21967437"
FT VARIANT 225
FT /note="V -> F (in strain: QB_2, TW18 and Anc)"
FT /evidence="ECO:0000269|PubMed:14667253,
FT ECO:0000269|PubMed:19956760, ECO:0000269|PubMed:25056887,
FT ECO:0000269|PubMed:7506687"
FT VARIANT 292
FT /note="V -> M (in strain: Qbeta_1_FR and Qbeta_3_FR)"
FT /evidence="ECO:0000269|PubMed:21967437"
FT STRAND 171..179
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 183..185
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 189..195
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 199..202
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 204..207
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 208..211
FT /evidence="ECO:0007829|PDB:3RLK"
FT TURN 219..222
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 233..235
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 238..241
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 244..249
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 254..258
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 260..263
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 266..272
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 273..275
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 277..280
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 281..284
FT /evidence="ECO:0007829|PDB:3RLK"
FT TURN 287..289
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 291..296
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 301..304
FT /evidence="ECO:0007829|PDB:3RLK"
FT HELIX 310..312
FT /evidence="ECO:0007829|PDB:3RLK"
FT TURN 315..317
FT /evidence="ECO:0007829|PDB:3RLK"
FT STRAND 320..325
FT /evidence="ECO:0007829|PDB:3RLK"
SQ SEQUENCE 329 AA; 36086 MW; F5F24701F984969B CRC64;
MAKLETVTLG NIGKDGKQTL VLNPRGVNPT NGVASLSQAG AVPALEKRVT VSVSQPSRNR
KNYKVQVKIQ NPTACTANGS CDPSVTRQAY ADVTFSFTQY STDEERAFVR TELAALLASP
LLIDAIDQLN PAYWTLLIAG GGSGSKPDPV IPDPPIDPPP GTGKYTCPFA IWSLEEVYEP
PTKNRPWPIY NAVELQPREF DVALKDLLGN TKWRDWDSRL SYTTVRGCRG NGYIDLDATY
LATDQAMRDQ KYDIREGKKP GAFGNIERFI YLKSINAYCS LSDIAAYHAD GVIVGFWRDP
SSGGAIPFDF TKFDKTKCPI QAVIVVPRA
