A1_BPT5
ID A1_BPT5 Reviewed; 556 AA.
AC Q6QGT3; Q5DMD9; Q66M45;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=Protein A1 {ECO:0000305};
GN Name=A1 {ECO:0000312|EMBL:AAS77051.1};
GN ORFNames=T5.004 {ECO:0000312|EMBL:AAS77051.1},
GN T5p004 {ECO:0000312|EMBL:AAU05157.1};
GN and
GN Name=A1.2 {ECO:0000312|EMBL:AAU05301.1};
GN ORFNames=T5p162 {ECO:0000312|EMBL:AAU05301.1};
OS Escherichia phage T5 (Enterobacteria phage T5).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX NCBI_TaxID=2695836;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12092.1};
RX PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA Hu S.;
RT "Complete genome sequence of bacteriophage T5.";
RL Virology 332:45-65(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT "Bacteriophage T5 complete genome.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=St0 deletion mutant {ECO:0000312|EMBL:AAU05301.1};
RX PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA Boulanger P.;
RT "Insights into bacteriophage T5 structure from analysis of its
RT morphogenesis genes and protein components.";
RL J. Virol. 88:1162-1174(2014).
RN [4]
RP FUNCTION.
RX PubMed=4915289; DOI=10.1016/0022-2836(70)90224-x;
RA McCorquodale D.J., Lanni Y.T.;
RT "Patterns of protein synthesis in Escherichia coli infected by amber
RT mutants in the first-step-transfer DNA of T5.";
RL J. Mol. Biol. 48:133-143(1970).
RN [5]
RP SUBUNIT.
RX PubMed=5136582; DOI=10.1016/0022-2836(71)90155-0;
RA Beckman L.D., Hoffman M.S., McCorquodale D.J.;
RT "Pre-early proteins of bacteriophage T5: structure and function.";
RL J. Mol. Biol. 62:551-564(1971).
RN [6]
RP INDUCTION.
RX PubMed=325230; DOI=10.1128/jvi.22.2.480-488.1977;
RA McCorquodale D.J., Shaw A.R., Shaw P.K., Chinnadurai G.;
RT "Pre-early polypeptides of bacteriophages T5 and BF23.";
RL J. Virol. 22:480-488(1977).
RN [7]
RP FUNCTION, AND IDENTIFICATION IN A COMPLEX WITH HOST RNA POLYMERASE.
RX PubMed=7033565; DOI=10.1128/jvi.40.3.958-962.1981;
RA McCorquodale D.J., Chen C.W., Joseph M.K., Woychik R.;
RT "Modification of RNA polymerase from Escherichia coli by pre-early gene
RT products of bacteriophage T5.";
RL J. Virol. 40:958-962(1981).
CC -!- FUNCTION: Involved in the degradation of host DNA and the shutoff of
CC host genes and phage pre-early genes. Together with A2 protein,
CC involved in the second step transfer (SST) of viral DNA into the host
CC cell, which leads to the complete entry of viral DNA into the host
CC cell. {ECO:0000269|PubMed:4915289, ECO:0000269|PubMed:7033565,
CC ECO:0000303|PubMed:15661140}.
CC -!- SUBUNIT: Homooligomer (PubMed:5136582). Interacts with A2 protein; the
CC two proteins form heterooligomers (PubMed:5136582). Part of a complex
CC containing the host RNA polymerase, protein A1 as well as another viral
CC protein of 11 kDa; these interactions are probably involved in the
CC transcription shutoff of pre-early genes (PubMed:7033565).
CC {ECO:0000269|PubMed:5136582, ECO:0000269|PubMed:7033565}.
CC -!- INDUCTION: Expressed pre-early in the viral replicative cycle.
CC Expressed with a few other proteins from the first step transfer (FST)
CC DNA. FST DNA corresponds to the first small portion of the genome that
CC enters into the host cell at the beginning of infection before pausing.
CC {ECO:0000269|PubMed:325230}.
CC -!- MISCELLANEOUS: This gene is part of the long terminal repeats present
CC at both ends of the viral genome and is thus duplicated.
CC {ECO:0000269|PubMed:15661140}.
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DR EMBL; AY587007; AAX11941.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY587007; AAX12092.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AY543070; AAS77051.1; -; Genomic_DNA.
DR EMBL; AY692264; AAU05301.1; -; Genomic_DNA.
DR EMBL; AY692264; AAU05157.1; -; Genomic_DNA.
DR RefSeq; YP_006832.1; NC_005859.1.
DR GeneID; 2777670; -.
DR KEGG; vg:2777670; -.
DR Proteomes; UP000002107; Genome.
DR Proteomes; UP000002141; Genome.
DR Proteomes; UP000002503; Genome.
DR GO; GO:0099015; P:degradation of host chromosome by virus; IEA:UniProtKB-KW.
DR GO; GO:0039657; P:suppression by virus of host gene expression; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Bacterial host gene expression shutoff by virus;
KW Degradation of host chromosome by virus; Early protein;
KW Host gene expression shutoff by virus; Host-virus interaction;
KW Reference proteome.
FT CHAIN 1..556
FT /note="Protein A1"
FT /id="PRO_0000433206"
SQ SEQUENCE 556 AA; 61463 MW; 56EBDDBBDD37E249 CRC64;
MVISAEKQTV ILKMAADFNF YGKRLRATKL EVCDDISKMV YDTTKHSTAI CDWLEANKPA
KPKAAKVAKA IKNDERPEAA GIVSSTVEQW EVKQGKRFII TSIQNNTFPH KNFLASLEQY
AQFIGADLLV SKFIYNKNGF QNGEGADGIR YDSAFDKYIC NKNVFLNNRR FAFMAEINVL
PTADYPLSGF AETATALNLE GLAIGAAKIT AESVPALKGE IVRRMYSTGT ATLKNYIQQK
AGQKAEALHN FGALIVEFDE DGEFFVRQLE TMDESGVFYD LNTCATPAGC YETTGHVLGL
QYGDIHAEKL DEECAAASWG HGDTYGLVDI LKPKYQFVHD VHDFTSRNHH NRASGVFLAK
QYAAGRDKVL DDLIDTGRVL ESMERDFSQT IIVESNHDLA LSRWLDDRNA NIKDDPANAE
LYHRLNAAIY GAIAEKDDTF NVLDYALRKV AGCEFNAIFL TTDQSFKIAG IECGVHGHNG
INGSRGNPKQ FKKLGKLNTG HTHTASIYGG VYTAGVTGSL DMGYNVGASS WTQTHIITYA
NGQRTLIDFK NGKFFA
