ARP1_METAQ
ID ARP1_METAQ Reviewed; 446 AA.
AC E9EHG0;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-APR-2011, sequence version 1.
DT 03-AUG-2022, entry version 36.
DE RecName: Full=Scytalone dehydratase-like protein Arp1 {ECO:0000305|PubMed:29958281};
DE EC=4.2.1.- {ECO:0000305|PubMed:29958281};
GN Name=Arp1 {ECO:0000303|PubMed:29958281}; ORFNames=MAC_09308;
OS Metarhizium acridum (strain CQMa 102).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium.
OX NCBI_TaxID=655827;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CQMa 102;
RX PubMed=21253567; DOI=10.1371/journal.pgen.1001264;
RA Gao Q., Jin K., Ying S.-H., Zhang Y., Xiao G., Shang Y., Duan Z., Hu X.,
RA Xie X.-Q., Zhou G., Peng G., Luo Z., Huang W., Wang B., Fang W., Wang S.,
RA Zhong Y., Ma L.-J., St Leger R.J., Zhao G.-P., Pei Y., Feng M.-G., Xia Y.,
RA Wang C.;
RT "Genome sequencing and comparative transcriptomics of the model
RT entomopathogenic fungi Metarhizium anisopliae and M. acridum.";
RL PLoS Genet. 7:E1001264-E1001264(2011).
RN [2]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=29958281; DOI=10.1371/journal.pgen.1007472;
RA Zeng G., Zhang P., Zhang Q., Zhao H., Li Z., Zhang X., Wang C., Yin W.B.,
RA Fang W.;
RT "Duplication of a Pks gene cluster and subsequent functional
RT diversification facilitate environmental adaptation in Metarhizium
RT species.";
RL PLoS Genet. 14:E1007472-E1007472(2018).
CC -!- FUNCTION: Scytalone dehydratase-like protein; part of the Pks2 gene
CC cluster that mediates the formation of infectious structures
CC (appressoria), enabling these fungi to kill insects faster
CC (PubMed:29958281). The product of the Pks2 gene cluster is different
CC from the one of Pks1 and has still not been identified
CC (PubMed:29958281). {ECO:0000269|PubMed:29958281}.
CC -!- SUBUNIT: Homotrimer. Each subunit contains an active site, located in
CC the central part of the hydrophobic core of the monomer, which
CC functions independently. {ECO:0000250|UniProtKB:P56221}.
CC -!- SIMILARITY: Belongs to the scytalone dehydratase family. {ECO:0000305}.
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DR EMBL; GL698613; EFY84643.1; -; Genomic_DNA.
DR RefSeq; XP_007815648.1; XM_007817457.1.
DR AlphaFoldDB; E9EHG0; -.
DR SMR; E9EHG0; -.
DR STRING; 92637.XP_007815648.1; -.
DR EnsemblFungi; EFY84643; EFY84643; MAC_09308.
DR GeneID; 19253619; -.
DR KEGG; maw:MAC_09308; -.
DR eggNOG; KOG1211; Eukaryota.
DR HOGENOM; CLU_614049_0_0_1; -.
DR OrthoDB; 1377897at2759; -.
DR Proteomes; UP000002499; Unassembled WGS sequence.
DR GO; GO:0030411; F:scytalone dehydratase activity; IEA:InterPro.
DR GO; GO:0006582; P:melanin metabolic process; IEA:InterPro.
DR Gene3D; 3.90.1300.10; -; 1.
DR InterPro; IPR000120; Amidase.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR004235; Scytalone_dehydratase.
DR PANTHER; PTHR11895; PTHR11895; 2.
DR Pfam; PF01425; Amidase; 1.
DR Pfam; PF02982; Scytalone_dh; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR SUPFAM; SSF75304; SSF75304; 1.
PE 3: Inferred from homology;
KW Lyase; Reference proteome.
FT CHAIN 1..446
FT /note="Scytalone dehydratase-like protein Arp1"
FT /id="PRO_0000445914"
FT ACT_SITE 358
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT ACT_SITE 383
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 323
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
FT BINDING 404
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P56221"
SQ SEQUENCE 446 AA; 48911 MW; D126AB256C5ADA6A CRC64;
MQNLTDKGAI IFGVQKTSQF ANGETATADW VDYHSPFNPR GDGYQDPATS SAGAGSSIAS
YEWLDLAAGS DTGGSIRGPA AVQGIFGNRP SHGLISLDNA MPLSPKLDTP GFLARDPYLW
NAANAALYRE NPLSRWSWGD SQPSSILSRN KTLFMNWFNE NILPRSSDPL TCSSGLLLHV
DGSAGFISRN GYINPPVPPF GFSNGQISVF AETPDSVFPL GQVPAFSSIT NHTEYLPVTI
NVVAAKGCDV LIAKLAEDMV AAGILKVLKI GAGIEGGEIL MRRYFVRDDG DEVMSRITSR
GRLLFQDWDR LRGILAPTLY VDYTKIGKGK WEAMSADDFM AMVSNDDFLG DLCVKTQHLI
GATSWERVSE SKVVGHHQLR AAHQVYTSPD LKTVKLRGHS HATNEHYYVK SDGVWKFAGL
KPEVRWNEHK FEEVFKGSYT QSEKHS