ID   ARP1_METMF              Reviewed;         174 AA.
AC   A0A0B4HM53;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   04-MAR-2015, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Scytalone dehydratase-like protein Arp1 {ECO:0000305|PubMed:29958281};
DE            EC=4.2.1.- {ECO:0000305|PubMed:29958281};
GN   Name=Arp1 {ECO:0000303|PubMed:29958281}; ORFNames=MAJ_07524;
OS   Metarhizium majus (strain ARSEF 297).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Clavicipitaceae; Metarhizium;
OC   Metarhizium majus.
OX   NCBI_TaxID=1276143;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ARSEF 297;
RX   PubMed=25368161; DOI=10.1073/pnas.1412662111;
RA   Hu X., Xiao G., Zheng P., Shang Y., Su Y., Zhang X., Liu X., Zhan S.,
RA   St Leger R.J., Wang C.;
RT   "Trajectory and genomic determinants of fungal-pathogen speciation and host
RT   adaptation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 111:16796-16801(2014).
RN   [2]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=29958281; DOI=10.1371/journal.pgen.1007472;
RA   Zeng G., Zhang P., Zhang Q., Zhao H., Li Z., Zhang X., Wang C., Yin W.B.,
RA   Fang W.;
RT   "Duplication of a Pks gene cluster and subsequent functional
RT   diversification facilitate environmental adaptation in Metarhizium
RT   species.";
RL   PLoS Genet. 14:E1007472-E1007472(2018).
CC   -!- FUNCTION: Scytalone dehydratase-like protein; part of the Pks2 gene
CC       cluster that mediates the formation of infectious structures
CC       (appressoria), enabling these fungi to kill insects faster
CC       (PubMed:29958281). The product of the Pks2 gene cluster is different
CC       from the one of Pks1 and has still not been identified
CC       (PubMed:29958281). {ECO:0000269|PubMed:29958281}.
CC   -!- SUBUNIT: Homotrimer. Each subunit contains an active site, located in
CC       the central part of the hydrophobic core of the monomer, which
CC       functions independently. {ECO:0000250|UniProtKB:P56221}.
CC   -!- SIMILARITY: Belongs to the scytalone dehydratase family. {ECO:0000305}.
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DR   EMBL; AZNE01000049; KID96468.1; -; Genomic_DNA.
DR   RefSeq; XP_014575462.1; XM_014719976.1.
DR   AlphaFoldDB; A0A0B4HM53; -.
DR   SMR; A0A0B4HM53; -.
DR   EnsemblFungi; KID96468; KID96468; MAJ_07524.
DR   HOGENOM; CLU_101889_1_0_1; -.
DR   OrthoDB; 1377897at2759; -.
DR   Proteomes; UP000031176; Unassembled WGS sequence.
DR   GO; GO:0030411; F:scytalone dehydratase activity; IEA:InterPro.
DR   GO; GO:0006582; P:melanin metabolic process; IEA:InterPro.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR004235; Scytalone_dehydratase.
DR   Pfam; PF02982; Scytalone_dh; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Lyase.
FT   CHAIN           1..174
FT                   /note="Scytalone dehydratase-like protein Arp1"
FT                   /id="PRO_0000445916"
FT   ACT_SITE        84
FT                   /evidence="ECO:0000250|UniProtKB:P56221"
FT   ACT_SITE        109
FT                   /evidence="ECO:0000250|UniProtKB:P56221"
FT   BINDING         49
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P56221"
FT   BINDING         130
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:P56221"
SQ   SEQUENCE   174 AA;  19752 MW;  05E92D849573F022 CRC64;
     MAQSLTPSVE TDSKTGPSKA ISFQDYLDLS ALDWARLRAV LAPTLYVDYT KIGKEKWDAM
     SADDFMAMVS NDDFLGDLCV KTQHLIGATY WERVSESKVI GHHQLRAAHQ VYTSPDLKTV
     KLRGHSHATN EHYYVKSGGV WKFAGLKPEV RWNEYKFEEV FKGSYTQSEK QSLP