ID   ARP2A_DANRE             Reviewed;         394 AA.
AC   Q7SXW6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Actin-related protein 2-A;
DE   AltName: Full=Actin-like protein 2-A;
GN   Name=actr2a; Synonyms=actr2, arp2a;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ATP-binding component of the Arp2/3 complex, a multiprotein
CC       complex that mediates actin polymerization upon stimulation by
CC       nucleation-promoting factor (NPF) (By similarity). The Arp2/3 complex
CC       mediates the formation of branched actin networks in the cytoplasm,
CC       providing the force for cell motility (By similarity). Seems to contact
CC       the pointed end of the daughter actin filament (By similarity). In
CC       addition to its role in the cytoplasmic cytoskeleton, the Arp2/3
CC       complex also promotes actin polymerization in the nucleus, thereby
CC       regulating gene transcription and repair of damaged DNA (By
CC       similarity). The Arp2/3 complex promotes homologous recombination (HR)
CC       repair in response to DNA damage by promoting nuclear actin
CC       polymerization, leading to drive motility of double-strand breaks
CC       (DSBs) (By similarity). {ECO:0000250|UniProtKB:P61160,
CC       ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of actr2/arp2,
CC       actr3/arp3, arpc1b, arpc2, arpc3, arpc4 and arpc5.
CC       {ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q7ZTP2}. Cell projection
CC       {ECO:0000250|UniProtKB:Q7ZTP2}. Nucleus {ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP2 subfamily. {ECO:0000305}.
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DR   EMBL; BC055220; AAH55220.1; -; mRNA.
DR   RefSeq; NP_998664.1; NM_213499.1.
DR   AlphaFoldDB; Q7SXW6; -.
DR   SMR; Q7SXW6; -.
DR   STRING; 7955.ENSDARP00000068783; -.
DR   PaxDb; Q7SXW6; -.
DR   GeneID; 406820; -.
DR   KEGG; dre:406820; -.
DR   CTD; 406820; -.
DR   ZFIN; ZDB-GENE-040426-2894; actr2a.
DR   eggNOG; KOG0677; Eukaryota.
DR   InParanoid; Q7SXW6; -.
DR   OrthoDB; 649708at2759; -.
DR   PhylomeDB; Q7SXW6; -.
DR   Reactome; R-DRE-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR   Reactome; R-DRE-3928662; EPHB-mediated forward signaling.
DR   Reactome; R-DRE-5663213; RHO GTPases Activate WASPs and WAVEs.
DR   Reactome; R-DRE-6798695; Neutrophil degranulation.
DR   Reactome; R-DRE-8856828; Clathrin-mediated endocytosis.
DR   PRO; PR:Q7SXW6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005885; C:Arp2/3 protein complex; ISS:UniProtKB.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
DR   GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR027306; Arp2.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; PTHR11937; 1.
DR   PANTHER; PTHR11937:SF149; PTHR11937:SF149; 1.
DR   Pfam; PF00022; Actin; 1.
DR   PRINTS; PR00190; ACTIN.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding; ATP-binding; Cell projection; Cytoplasm; Cytoskeleton;
KW   Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..394
FT                   /note="Actin-related protein 2-A"
FT                   /id="PRO_0000327247"
FT   BINDING         160..162
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
FT   BINDING         214..218
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
FT   BINDING         305..310
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
SQ   SEQUENCE   394 AA;  44622 MW;  88BD334684ED8E69 CRC64;
     MDSQGKKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTAKVGNI EIKDLMVGDE
     ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DSRNCKILLT EPPMNPTKNR
     EKIIEVMFET YQFSGVYIAI QAVLTLYAQG LLTGVVVDSG DGVTHICPVY EGFSLPHLTR
     RLDIAGRDIT RYLIKLLLLR GYAFNHSADF ETVRMMKEKL CYVGYNIEQE QKLALETTVL
     VESYTLPDGR VIKVGGERFG APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRAEFYKH
     IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVDKLSKF KIRIEDPPRR KHMVFLGGAV
     LADIMKDKDN FWMTREEYQE KGTRVLEKLG VTVR