ID   ARP2_CHICK              Reviewed;         394 AA.
AC   P53488;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Actin-related protein 2;
DE   AltName: Full=Actin-like protein 2;
DE   AltName: Full=Actin-like protein ACTL;
GN   Name=ACTR2; Synonyms=ARP2;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7890165; DOI=10.1016/0378-1119(94)00865-p;
RA   Michaille J.J., Gouy M., Blanchet S., Duret L.;
RT   "Isolation and characterization of a cDNA encoding a chicken actin-like
RT   protein.";
RL   Gene 154:205-209(1995).
CC   -!- FUNCTION: ATP-binding component of the Arp2/3 complex, a multiprotein
CC       complex that mediates actin polymerization upon stimulation by
CC       nucleation-promoting factor (NPF) (By similarity). The Arp2/3 complex
CC       mediates the formation of branched actin networks in the cytoplasm,
CC       providing the force for cell motility (By similarity). Seems to contact
CC       the pointed end of the daughter actin filament (By similarity). In
CC       addition to its role in the cytoplasmic cytoskeleton, the Arp2/3
CC       complex also promotes actin polymerization in the nucleus, thereby
CC       regulating gene transcription and repair of damaged DNA (By
CC       similarity). The Arp2/3 complex promotes homologous recombination (HR)
CC       repair in response to DNA damage by promoting nuclear actin
CC       polymerization, leading to drive motility of double-strand breaks
CC       (DSBs) (By similarity). {ECO:0000250|UniProtKB:P61160,
CC       ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of ACTR2/ARP2,
CC       ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC
CC       and ARPC5/p16-ARC. {ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q7ZTP2}. Cell projection
CC       {ECO:0000250|UniProtKB:Q7ZTP2}. Nucleus {ECO:0000250|UniProtKB:Q7ZTP2}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP2 subfamily. {ECO:0000305}.
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DR   EMBL; X73971; CAA52151.1; -; mRNA.
DR   PIR; JC4039; JC4039.
DR   RefSeq; NP_990555.1; NM_205224.1.
DR   AlphaFoldDB; P53488; -.
DR   SMR; P53488; -.
DR   STRING; 9031.ENSGALP00000014260; -.
DR   PaxDb; P53488; -.
DR   PRIDE; P53488; -.
DR   Ensembl; ENSGALT00000093476; ENSGALP00000070985; ENSGALG00000008779.
DR   GeneID; 396147; -.
DR   KEGG; gga:396147; -.
DR   CTD; 10097; -.
DR   VEuPathDB; HostDB:geneid_396147; -.
DR   eggNOG; KOG0677; Eukaryota.
DR   GeneTree; ENSGT00940000154556; -.
DR   HOGENOM; CLU_027965_0_0_1; -.
DR   InParanoid; P53488; -.
DR   OMA; WEDMQHL; -.
DR   OrthoDB; 649708at2759; -.
DR   PhylomeDB; P53488; -.
DR   PRO; PR:P53488; -.
DR   Proteomes; UP000000539; Chromosome 3.
DR   Bgee; ENSGALG00000008779; Expressed in spleen and 14 other tissues.
DR   ExpressionAtlas; P53488; baseline and differential.
DR   GO; GO:0005885; C:Arp2/3 protein complex; ISS:UniProtKB.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
DR   GO; GO:1905168; P:positive regulation of double-strand break repair via homologous recombination; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; PTHR11937; 1.
DR   Pfam; PF00022; Actin; 1.
DR   PRINTS; PR00190; ACTIN.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Actin-binding; ATP-binding; Cell projection; Cytoplasm; Cytoskeleton;
KW   Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..394
FT                   /note="Actin-related protein 2"
FT                   /id="PRO_0000089071"
FT   BINDING         160..162
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
FT   BINDING         214..218
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
FT   BINDING         305..310
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250|UniProtKB:A7MB62"
SQ   SEQUENCE   394 AA;  44702 MW;  827564C4F213E934 CRC64;
     MDTLGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTAKVGNI EIKDLMVGDE
     ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DTKNCKILLT EPPMNPTKNR
     EKIVEVMFET YQFSGVYVAI QAVLTLYAQG LLTGVVVDSG DGVTHICPVY EGFSLPHLTR
     RLDIAGRDIT RYLIKLLLLR GYAFNHSADF ETVRMIKEKL CYVGYNIEQE QKLALETTVL
     VESYTLPDGR IIKVGGERFE APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRSEFYKH
     IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVEKLSKF KIRIEDPPRR KHMVFLGGAV
     LADIMKDKDN FWMTRQEYQE KGVRVLEKLG VTVR