ID   NUOJ_RHOCA              Reviewed;         202 AA.
AC   P50975;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=NADH-quinone oxidoreductase subunit J;
DE            EC=7.1.1.-;
DE   AltName: Full=NADH dehydrogenase I subunit J;
DE   AltName: Full=NDH-1 subunit J;
GN   Name=nuoJ;
OS   Rhodobacter capsulatus (Rhodopseudomonas capsulata).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC   Rhodobacteraceae; Rhodobacter.
OX   NCBI_TaxID=1061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=8566820; DOI=10.1016/0378-1119(95)00693-1;
RA   Dupuis A., Peinnequin A., Chevallet M., Lunardi J., Darrouzet E.,
RA   Pierrard B., Procaccio V., Issartel J.P.;
RT   "Identification of five Rhodobacter capsulatus genes encoding the
RT   equivalent of ND subunits of the mitochondrial NADH-ubiquinone
RT   oxidoreductase.";
RL   Gene 167:99-104(1995).
RN   [2]
RP   SUBCELLULAR LOCATION IN CHROMATOPHORE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33303 / B10;
RX   PubMed=9632256; DOI=10.1046/j.1365-2958.1998.00814.x;
RA   Dupuis A., Darrouzet E., Duborjal H., Pierrard B., Chevallet M.,
RA   van Belzen R., Albracht S.P.J., Lunardi J.;
RT   "Distal genes of the nuo operon of Rhodobacter capsulatus equivalent to the
RT   mitochondrial ND subunits are all essential for the biogenesis of the
RT   respiratory NADH-ubiquinone oxidoreductase.";
RL   Mol. Microbiol. 28:531-541(1998).
CC   -!- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur
CC       (Fe-S) centers, to quinones in the respiratory chain. The immediate
CC       electron acceptor for the enzyme in this species is believed to be
CC       ubiquinone. Couples the redox reaction to proton translocation (for
CC       every two electrons transferred, four hydrogen ions are translocated
CC       across the cytoplasmic membrane), and thus conserves the redox energy
CC       in a proton gradient.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
CC   -!- SUBUNIT: NDH-1 is composed of 14 different subunits. Subunits NuoA, H,
CC       J, K, L, M, N constitute the membrane sector of the complex (Probable).
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Cellular chromatophore membrane
CC       {ECO:0000269|PubMed:9632256}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:9632256}.
CC   -!- DISRUPTION PHENOTYPE: No functional NADH-quinone oxidoreductase
CC       complex. Cells lacking this gene have a nearly normal respiratory
CC       growth phenotype on lactate, however they are unable to perform
CC       anaerobic photosynthesis. It is suggested that in R.capsulatus this
CC       complex may function in reverse flow under physiological conditions.
CC       {ECO:0000269|PubMed:9632256}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 6 family. {ECO:0000305}.
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DR   EMBL; AF029365; AAC25001.1; -; Genomic_DNA.
DR   AlphaFoldDB; P50975; -.
DR   SMR; P50975; -.
DR   GeneID; 31490413; -.
DR   OMA; MLVNKRE; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042717; C:plasma membrane-derived chromatophore membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.1200; -; 1.
DR   InterPro; IPR001457; NADH_UbQ/plastoQ_OxRdtase_su6.
DR   InterPro; IPR042106; Nuo/plastoQ_OxRdtase_6_NuoJ.
DR   Pfam; PF00499; Oxidored_q3; 1.
PE   3: Inferred from homology;
KW   Membrane; NAD; Quinone; Translocase; Transmembrane; Transmembrane helix;
KW   Ubiquinone.
FT   CHAIN           1..202
FT                   /note="NADH-quinone oxidoreductase subunit J"
FT                   /id="PRO_0000118377"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..166
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   202 AA;  21733 MW;  C020DA24BEDDCB9F CRC64;
     MTVFVFAFYL FAVVAVVAGL MVVLSKNPVH AVLWLILTFL SAAGLFVLMG AEFVAMLLII
     VYVGAVAVLF LFVVMMLDID FAALRGQLVR YAPVGGLIAL VMLAQLATGL MVWNTADAAN
     GLRAAPAPEG VENTAALGMI LYDRYLYLFQ GAGLVLLVAM IGAILLTLRH RTDIKRQNVL
     AQMHRDPAKA LEMVDVKPGQ GL