ID   ARP3_CHICK              Reviewed;         418 AA.
AC   Q90WD0;
DT   01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Actin-related protein 3;
DE   AltName: Full=Actin-like protein 3;
GN   Name=ACTR3; Synonyms=ARP3;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Brain;
RX   PubMed=15923655; DOI=10.1242/jcs.02371;
RA   Mingle L.A., Okuhama N.N., Shi J., Singer R.H., Condeelis J., Liu G.;
RT   "Localization of all seven messenger RNAs for the actin-polymerization
RT   nucleator Arp2/3 complex in the protrusions of fibroblasts.";
RL   J. Cell Sci. 118:2425-2433(2005).
CC   -!- FUNCTION: ATP-binding component of the Arp2/3 complex, a multiprotein
CC       complex that mediates actin polymerization upon stimulation by
CC       nucleation-promoting factor (NPF). The Arp2/3 complex mediates the
CC       formation of branched actin networks in the cytoplasm, providing the
CC       force for cell motility (By similarity). Seems to contact the pointed
CC       end of the daughter actin filament (By similarity). In addition to its
CC       role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes
CC       actin polymerization in the nucleus, thereby regulating gene
CC       transcription and repair of damaged DNA (By similarity). The Arp2/3
CC       complex promotes homologous recombination (HR) repair in response to
CC       DNA damage by promoting nuclear actin polymerization, leading to drive
CC       motility of double-strand breaks (DSBs) (By similarity).
CC       {ECO:0000250|UniProtKB:P61158, ECO:0000250|UniProtKB:Q801P7}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of ACTR2/ARP2,
CC       ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC
CC       and ARPC5/p16-ARC. {ECO:0000250|UniProtKB:Q801P7}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC       {ECO:0000250|UniProtKB:Q801P7}. Cell projection
CC       {ECO:0000250|UniProtKB:Q801P7}. Nucleus {ECO:0000250|UniProtKB:P61158}.
CC   -!- TISSUE SPECIFICITY: Detected in fibroblasts.
CC       {ECO:0000269|PubMed:15923655}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP3 subfamily. {ECO:0000305}.
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DR   EMBL; AF288600; AAK83060.1; -; mRNA.
DR   RefSeq; NP_989638.1; NM_204307.1.
DR   AlphaFoldDB; Q90WD0; -.
DR   SMR; Q90WD0; -.
DR   STRING; 9031.ENSGALP00000019832; -.
DR   PaxDb; Q90WD0; -.
DR   Ensembl; ENSGALT00000019859; ENSGALP00000019832; ENSGALG00000012159.
DR   GeneID; 374197; -.
DR   KEGG; gga:374197; -.
DR   CTD; 10096; -.
DR   VEuPathDB; HostDB:geneid_374197; -.
DR   eggNOG; KOG0678; Eukaryota.
DR   GeneTree; ENSGT00940000155065; -.
DR   HOGENOM; CLU_027965_3_0_1; -.
DR   InParanoid; Q90WD0; -.
DR   OMA; TAEFKSY; -.
DR   OrthoDB; 649708at2759; -.
DR   PhylomeDB; Q90WD0; -.
DR   TreeFam; TF300644; -.
DR   Reactome; R-GGA-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR   Reactome; R-GGA-3928662; EPHB-mediated forward signaling.
DR   Reactome; R-GGA-5663213; RHO GTPases Activate WASPs and WAVEs.
DR   Reactome; R-GGA-8856828; Clathrin-mediated endocytosis.
DR   PRO; PR:Q90WD0; -.
DR   Proteomes; UP000000539; Chromosome 7.
DR   Bgee; ENSGALG00000012159; Expressed in spleen and 14 other tissues.
DR   GO; GO:0005885; C:Arp2/3 protein complex; ISS:UniProtKB.
DR   GO; GO:0042995; C:cell projection; IEA:UniProtKB-SubCell.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0035861; C:site of double-strand break; ISS:UniProtKB.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR015623; Arp3.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; PTHR11937; 1.
DR   PANTHER; PTHR11937:SF175; PTHR11937:SF175; 1.
DR   Pfam; PF00022; Actin; 1.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Actin-binding; ATP-binding; Cell projection; Cytoplasm;
KW   Cytoskeleton; Nucleotide-binding; Nucleus; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..418
FT                   /note="Actin-related protein 3"
FT                   /id="PRO_0000342357"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   418 AA;  47421 MW;  832BDBB14FCAFED7 CRC64;
     MAGRLPACVV DCGTGYTKLG YAGNTEPQFI IPSCIAIKES AKVVDQAQRR VLKGVDDLDF
     FIGDEAIEKP TYATKWPIRH GIVEDWDLME RFMEQVIFKY LRAEPEDHYF LLTEPPLNTP
     ENREYTAEIM FESFNVPGLY IAVQAVLALA ASWTSRQVGE RTLTGTVIDS GDGVTHVIPV
     AEGYVIGSCI KHIPIAGRDI TYFIQQLLRE REVGIPPEQS LETAKAVKER FSYVCPDLVK
     EFNKYDTDGT KWIKQYTGIN AISKKEFTID VGYERFLGPE IFFHPEFANP DFTQPISEVV
     DEVIQNCPID VRRPLYKNIV LSGGSTMFRD FGRRLQRDLK RTVDARLKLS EELSGGRLKP
     KPIDVQVITH HMQRYAVWFG GSMLASTPEF YQVCHTKKDY EEIGPSICRH NPVFGVMS