NUOM_PSEAE
ID NUOM_PSEAE Reviewed; 509 AA.
AC Q9I0J0;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NADH-quinone oxidoreductase subunit M;
DE EC=7.1.1.-;
DE AltName: Full=NADH dehydrogenase I subunit M;
DE AltName: Full=NDH-1 subunit M;
GN Name=nuoM; OrderedLocusNames=PA2648;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur
CC (Fe-S) centers, to quinones in the respiratory chain. The immediate
CC electron acceptor for the enzyme in this species is believed to be
CC ubiquinone. Couples the redox reaction to proton translocation (for
CC every two electrons transferred, four hydrogen ions are translocated
CC across the cytoplasmic membrane), and thus conserves the redox energy
CC in a proton gradient (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) +
CC NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
CC -!- SUBUNIT: Composed of 13 different subunits. Subunits NuoA, H, J, K, L,
CC M, N constitute the membrane sector of the complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the complex I subunit 4 family. {ECO:0000305}.
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DR EMBL; AE004091; AAG06036.1; -; Genomic_DNA.
DR PIR; E83315; E83315.
DR RefSeq; NP_251338.1; NC_002516.2.
DR RefSeq; WP_003097684.1; NZ_QZGE01000008.1.
DR AlphaFoldDB; Q9I0J0; -.
DR SMR; Q9I0J0; -.
DR STRING; 287.DR97_5314; -.
DR PaxDb; Q9I0J0; -.
DR PRIDE; Q9I0J0; -.
DR DNASU; 882357; -.
DR EnsemblBacteria; AAG06036; AAG06036; PA2648.
DR GeneID; 882357; -.
DR KEGG; pae:PA2648; -.
DR PATRIC; fig|208964.12.peg.2771; -.
DR PseudoCAP; PA2648; -.
DR HOGENOM; CLU_007100_4_4_6; -.
DR InParanoid; Q9I0J0; -.
DR OMA; ITRWGNQ; -.
DR PhylomeDB; Q9I0J0; -.
DR BioCyc; PAER208964:G1FZ6-2688-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0045272; C:plasma membrane respiratory chain complex I; IBA:GO_Central.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0048039; F:ubiquinone binding; IBA:GO_Central.
DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central.
DR GO; GO:0042773; P:ATP synthesis coupled electron transport; IEA:InterPro.
DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central.
DR InterPro; IPR010227; NADH_Q_OxRdtase_chainM/4.
DR InterPro; IPR003918; NADH_UbQ_OxRdtase.
DR InterPro; IPR001750; ND/Mrp_mem.
DR PANTHER; PTHR43507; PTHR43507; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR PRINTS; PR01437; NUOXDRDTASE4.
DR TIGRFAMs; TIGR01972; NDH_I_M; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; NAD; Quinone;
KW Reference proteome; Translocase; Transmembrane; Transmembrane helix;
KW Ubiquinone.
FT CHAIN 1..509
FT /note="NADH-quinone oxidoreductase subunit M"
FT /id="PRO_0000287830"
FT TRANSMEM 1..21
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..143
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 316..336
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 467..487
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 509 AA; 55704 MW; E502ABB2EC8A293C CRC64;
MILPWLILIP FIGGFLCWIA EHSSKTLPRW IALLSMTLVL ILSLWIWATG DFQLAPAPGG
EPEWTLQFKV LWIERLGISI HLAMDGLSLL MVALTGLLGV LSVLCSWNEI QRRIGFFHLN
LLWILGGVIG VFLAIDLFLF FFFWEMMLVP MYFLIALWGH SSDDGKKTRI YAATKFFIFT
QASGLVMLVA ILGLVFVNFN ATGVITFDYA TLLKTQLSPH VEWLLMLGFF VAFAVKMPVV
PVHSWLPDAH AQAPTAGSVD LAGILLKTAA YGLIRFALPL FPNASAEFAP IAMWLGIIGI
FYGALLSFAQ TDIKRLVAYS SVSHMGFVMI GIYSGSQVAL QGVVVQMIAH GLSAAALFIL
CGQLYERLHT RDMRKMGGLW SRMPYLPAIS LFFASASLGL PGTGNFVGEF LILIGAFKVV
PVIIVIATFG LVFASVYSLI MIHRAYFGPS QSDEPILGLD ARELSMVLGL AVLLVLLGVY
PQPVLDISAA SMHGVQQWLG AALSTLAGR
