NUP1_ARATH
ID NUP1_ARATH Reviewed; 1309 AA.
AC Q9CAF4; Q9LPN4;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Nuclear pore complex protein NUP1 {ECO:0000303|PubMed:19843313};
DE AltName: Full=Nuclear pore complex protein NUP136 {ECO:0000303|PubMed:21189294};
DE AltName: Full=Nucleoporin 1 {ECO:0000303|PubMed:19843313};
DE AltName: Full=Nucleoporin 136 {ECO:0000303|PubMed:21189294};
GN Name=NUP1 {ECO:0000303|PubMed:19843313};
GN Synonyms=NUP136 {ECO:0000303|PubMed:21189294};
GN OrderedLocusNames=At3g10650 {ECO:0000312|Araport:AT3G10650};
GN ORFNames=F13M14.6 {ECO:0000312|EMBL:AAG51374.1},
GN F18K10.27 {ECO:0000312|EMBL:AAF76371.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION IN THE NUCLEAR PORE COMPLEX BY MASS SPECTROMETRY,
RP SUBCELLULAR LOCATION, FUNCTION, DISRUPTION PHENOTYPE, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=21189294; DOI=10.1105/tpc.110.079947;
RA Tamura K., Fukao Y., Iwamoto M., Haraguchi T., Hara-Nishimura I.;
RT "Identification and characterization of nuclear pore complex components in
RT Arabidopsis thaliana.";
RL Plant Cell 22:4084-4097(2010).
RN [5]
RP FUNCTION, INTERACTION WITH EER5, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=19843313; DOI=10.1111/j.1365-313x.2009.04048.x;
RA Lu Q., Tang X., Tian G., Wang F., Liu K., Nguyen V., Kohalmi S.E.,
RA Keller W.A., Tsang E.W., Harada J.J., Rothstein S.J., Cui Y.;
RT "Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components
RT and anchoring nucleoporin.";
RL Plant J. 61:259-270(2010).
RN [6]
RP FUNCTION.
RX PubMed=21818409; DOI=10.4161/nucl.2.3.16175;
RA Tamura K., Hara-Nishimura I.;
RT "Involvement of the nuclear pore complex in morphology of the plant
RT nucleus.";
RL Nucleus 2:168-172(2011).
RN [7]
RP INTERACTION WITH UCH1 AND UCH2.
RX PubMed=22951400; DOI=10.4161/psb.21899;
RA Tian G., Lu Q., Kohalmi S.E., Rothstein S.J., Cui Y.;
RT "Evidence that the Arabidopsis Ubiquitin C-terminal Hydrolases 1 and 2
RT associate with the 26S proteasome and the TREX-2 complex.";
RL Plant Signal. Behav. 7:1415-1419(2012).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=25081480; DOI=10.1126/science.1253736;
RA Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT culminating in enucleation.";
RL Science 345:933-937(2014).
CC -!- FUNCTION: Nucleoporin required for nuclear mRNA export. Functions as an
CC adapter and/or regulator molecule in the periphery of the nuclear pore
CC complex (NPC). May interact with importin proteins and mediate active
CC nucleocytoplasmic transport through the NPC. Involved in regulation of
CC nuclear morphology. {ECO:0000269|PubMed:19843313,
CC ECO:0000269|PubMed:21818409, ECO:0000305|PubMed:21189294}.
CC -!- SUBUNIT: Part of the nuclear pore complex (NPC). The NPC has an eight-
CC fold symmetrical structure comprising a central transport channel and
CC two rings, the cytoplasmic and nuclear rings, to which eight filaments
CC are attached. The cytoplasmic filaments have loose ends, while the
CC nuclear filaments are joined in a distal ring, forming a nuclear
CC basket. NPCs are highly dynamic in configuration and composition, and
CC can be devided in 3 subcomplexes, the NUP62 subcomplex, the NUP107-160
CC subcomplex and the NUP93 subcomplex, containing approximately 30
CC different nucleoporin proteins. Interacts with EER5, anchoring the
CC TREX-2 complex on the nuclear pore complex (PubMed:19843313). Interacts
CC with UCH1 and UCH2 (PubMed:22951400). {ECO:0000269|PubMed:19843313,
CC ECO:0000269|PubMed:22951400, ECO:0000305|PubMed:21189294}.
CC -!- INTERACTION:
CC Q9CAF4; Q8GWE6: EER5; NbExp=3; IntAct=EBI-2619711, EBI-2619706;
CC -!- SUBCELLULAR LOCATION: Nucleus envelope {ECO:0000269|PubMed:19843313,
CC ECO:0000269|PubMed:21189294, ECO:0000269|PubMed:25081480}. Nucleus,
CC nuclear pore complex {ECO:0000269|PubMed:21189294}. Cytoplasm, cytosol
CC {ECO:0000269|PubMed:21189294}. Note=Interacts dynamically with the NPC
CC and localizes to the cytosolic pool following nuclear envelope
CC breakdown during mitosis. {ECO:0000269|PubMed:21189294}.
CC -!- DOMAIN: Contains FG repeats mediating the translocation through the NPC
CC by interacting with transport factors. {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Developmental defects, early flowering and
CC altered nuclear morphology. Lethal when homozygous.
CC {ECO:0000269|PubMed:19843313, ECO:0000269|PubMed:21189294}.
CC -!- MISCELLANEOUS: Represents a functional homolog of vertebrate Nup153.
CC {ECO:0000305|PubMed:21189294}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF76371.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011560; AAG51374.1; -; Genomic_DNA.
DR EMBL; AC013428; AAF76371.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74939.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM65285.1; -; Genomic_DNA.
DR EMBL; AY093026; AAM13025.1; -; mRNA.
DR EMBL; BT010595; AAQ89617.1; -; mRNA.
DR RefSeq; NP_001327263.1; NM_001337888.1.
DR RefSeq; NP_187676.1; NM_111901.4.
DR AlphaFoldDB; Q9CAF4; -.
DR BioGRID; 5568; 62.
DR IntAct; Q9CAF4; 1.
DR STRING; 3702.AT3G10650.1; -.
DR iPTMnet; Q9CAF4; -.
DR PaxDb; Q9CAF4; -.
DR PRIDE; Q9CAF4; -.
DR ProteomicsDB; 249353; -.
DR EnsemblPlants; AT3G10650.1; AT3G10650.1; AT3G10650.
DR EnsemblPlants; AT3G10650.2; AT3G10650.2; AT3G10650.
DR GeneID; 820234; -.
DR Gramene; AT3G10650.1; AT3G10650.1; AT3G10650.
DR Gramene; AT3G10650.2; AT3G10650.2; AT3G10650.
DR KEGG; ath:AT3G10650; -.
DR Araport; AT3G10650; -.
DR TAIR; locus:2075785; AT3G10650.
DR eggNOG; KOG0845; Eukaryota.
DR HOGENOM; CLU_006995_1_0_1; -.
DR InParanoid; Q9CAF4; -.
DR OMA; MAEDTDQ; -.
DR OrthoDB; 395110at2759; -.
DR PhylomeDB; Q9CAF4; -.
DR PRO; PR:Q9CAF4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9CAF4; baseline and differential.
DR Genevisible; Q9CAF4; AT.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005635; C:nuclear envelope; IDA:TAIR.
DR GO; GO:0034399; C:nuclear periphery; IDA:TAIR.
DR GO; GO:0005643; C:nuclear pore; IDA:TAIR.
DR GO; GO:0071763; P:nuclear membrane organization; IBA:GO_Central.
DR GO; GO:0006997; P:nucleus organization; IMP:TAIR.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IMP:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cytoplasm; mRNA transport; Nuclear pore complex; Nucleus;
KW Protein transport; Reference proteome; Repeat; Translocation; Transport.
FT CHAIN 1..1309
FT /note="Nuclear pore complex protein NUP1"
FT /id="PRO_0000431091"
FT REPEAT 711..712
FT /note="1"
FT REPEAT 804..805
FT /note="2"
FT REPEAT 831..832
FT /note="3"
FT REPEAT 861..862
FT /note="4"
FT REPEAT 869..870
FT /note="5"
FT REPEAT 883..884
FT /note="6"
FT REPEAT 898..899
FT /note="7"
FT REPEAT 927..928
FT /note="8"
FT REPEAT 956..957
FT /note="9"
FT REPEAT 983..984
FT /note="10"
FT REPEAT 1004..1005
FT /note="11"
FT REPEAT 1029..1030
FT /note="12"
FT REPEAT 1038..1039
FT /note="13"
FT REPEAT 1053..1054
FT /note="14"
FT REPEAT 1089..1090
FT /note="15"
FT REPEAT 1121..1122
FT /note="16"
FT REPEAT 1137..1138
FT /note="17"
FT REPEAT 1151..1152
FT /note="18"
FT REPEAT 1153..1154
FT /note="19"
FT REPEAT 1166..1167
FT /note="20"
FT REPEAT 1177..1178
FT /note="21"
FT REPEAT 1186..1187
FT /note="22"
FT REPEAT 1224..1225
FT /note="23"
FT REPEAT 1238..1239
FT /note="24"
FT REPEAT 1255..1256
FT /note="25"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2..677
FT /note="25 X 2 AA repeats of F-G"
FT REGION 83..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..210
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..296
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 329..348
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..417
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 467..538
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 594..617
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 719..865
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1004..1028
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1068..1105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1278..1309
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 87..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 474..516
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 635..660
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 721..832
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 840..865
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1309 AA; 135933 MW; C95FAE517A250557 CRC64;
MASAARGESS NPYGGGLGTG GKFRKPTARR SQKTPYDRPT TSVRNAGLGG GDVRGGGWLS
KLVDPAQRLI TYSAQRLFGS LSRKRLGSGE TPLQSPEQQK QLPERGVNQE TKVGHKEDVS
NLSMKNGLIR MEDTNASVDP PKDGFTDLEK ILQGKTFTRS EVDRLTTLLR SKAADSSTMN
EEQRNEVGMV VRHPPSHERD RTHPDNGSMN TLVSTPPGSL RTLDECIASP AQLAKAYMGS
RPSEVTPSML GLRGQAGRED SVFLNRTPFP QKSPTMSLVT KPSGQRPLEN GFVTPRSRGR
SAVYSMARTP YSRPQSSVKI GSLFQASPSK WEESLPSGSR QGFQSGLKRR SSVLDNDIGS
VGPVRRIRQK SNLSSRSLAL PVSESPLSVR ANGGEKTTHT SKDSAEDIPG SSFNLVPTKS
SEMASKILQQ LDKLVSTREK SPSKLSPSML RGPALKSLQN VEAPKFLGNL PEKKANSPDS
SYQKQEISRE SVSREVLAQS EKTGDAVDGT SKTGSSKDQD MRGKGVYMPL TNSLEEHPPK
KRSFRMSAHE DFLELDDDLG AASTPCEVAE KQNAFEVEKS HISMPIGEKP LTPSEAMPST
SYISNGDASQ GTSNGSLETE RNKFVAFPIE AVQQSNMASE PTSKFIQGTE KSSISSGKPT
SEEKRIPLEE PKKPAAVFPN ISFSPPATGL LNQNSGASAD IKLEKTSSTA FGVSEAWAKP
TESKKTFSNS ASGAESSTSA APTLNGSIFS AGANAVTPPP SNGSLTSSPS FPPSISNIPS
DNSVGDMPST VQSFAATHNS SSIFGKLPTS NDSNSQSTSA SPLSSTSPFK FGQPAAPFSA
PAVSESSGQI SKETEVKNAT FGNTSTFKFG GMASADQSTG IVFGAKSAEN KSRPGFVFGS
SSVVGGSTLN PSTAAASAPE SSGSLIFGVT SSSTPGTETS KISASSAATN TGNSVFGTSS
FAFTSSGSSM VGGVSASTGS SVFGFNAVSS ASATSSQSQA SNLFGAGNAQ TGNTGSGTTT
STQSIPFQFG SSPSAPSFGL SGNSSLASNS SPFGFSKSEP AVFTSVSTPQ LSSTNSSASS
SSTMSSPLFG TSWQAPNSSP NSGPVFSSSF TTSSTPTTFS FGGSSAATVS STTTPIFGAS
TNNTPSPSPI FGFGSTPPTT PQQPVFGNSG TPSQSLFGNS TPGFAFGAPN NGNGINNNQQ
VSMEDSMAED TDQANRASMV APMFGQAAVS MPQPNFAFGG GAATQPPSMA NPFQFGGQPM
ASTLQNASPF QASQSLEFQG GGSFSLGSTG GGDKSGRRIF KAKKSTRKK
