NUP35_DROME
ID NUP35_DROME Reviewed; 331 AA.
AC Q9VWS2;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Nucleoporin Nup35 {ECO:0000305};
DE AltName: Full=35 kDa nucleoporin {ECO:0000305};
DE AltName: Full=Nuclear pore complex protein Nup35 {ECO:0000312|FlyBase:FBgn0030943};
DE AltName: Full=Nucleoporin Nup53 {ECO:0000303|PubMed:22718353};
GN Name=Nup35 {ECO:0000312|FlyBase:FBgn0030943};
GN Synonyms=Nup53 {ECO:0000303|PubMed:22718353};
GN ORFNames=CG6540 {ECO:0000312|FlyBase:FBgn0030943};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL28866.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH NUP154.
RX PubMed=22718353; DOI=10.1242/jcs.105809;
RA Busayavalasa K., Chen X., Farrants A.K., Wagner N., Sabri N.;
RT "The Nup155-mediated organisation of inner nuclear membrane proteins is
RT independent of Nup155 anchoring to the metazoan nuclear pore complex.";
RL J. Cell Sci. 125:4214-4218(2012).
CC -!- FUNCTION: Functions as a component of the nuclear pore complex (NPC)
CC (By similarity). May have a role in the organization of the inner
CC nuclear membrane proteins at the nuclear envelope together with Nup154
CC (PubMed:22718353). {ECO:0000250|UniProtKB:Q8NFH5,
CC ECO:0000269|PubMed:22718353}.
CC -!- SUBUNIT: Interacts with Nup154. {ECO:0000269|PubMed:22718353}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000255|PIRNR:PIRNR038119}.
CC -!- SIMILARITY: Belongs to the Nup35 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF48866.1; -; Genomic_DNA.
DR EMBL; AY061318; AAL28866.1; -; mRNA.
DR RefSeq; NP_573314.1; NM_133086.4.
DR AlphaFoldDB; Q9VWS2; -.
DR SMR; Q9VWS2; -.
DR IntAct; Q9VWS2; 7.
DR MINT; Q9VWS2; -.
DR STRING; 7227.FBpp0074372; -.
DR PaxDb; Q9VWS2; -.
DR PRIDE; Q9VWS2; -.
DR DNASU; 32851; -.
DR EnsemblMetazoa; FBtr0074601; FBpp0074372; FBgn0030943.
DR GeneID; 32851; -.
DR KEGG; dme:Dmel_CG6540; -.
DR UCSC; CG6540-RA; d. melanogaster.
DR CTD; 129401; -.
DR FlyBase; FBgn0030943; Nup35.
DR VEuPathDB; VectorBase:FBgn0030943; -.
DR eggNOG; KOG4285; Eukaryota.
DR GeneTree; ENSGT00390000005923; -.
DR HOGENOM; CLU_056189_0_0_1; -.
DR InParanoid; Q9VWS2; -.
DR OMA; FWVTIFG; -.
DR OrthoDB; 1163548at2759; -.
DR PhylomeDB; Q9VWS2; -.
DR Reactome; R-DME-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-DME-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-DME-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-DME-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DME-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-DME-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-DME-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-DME-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-DME-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-DME-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-DME-5578749; Transcriptional regulation by small RNAs.
DR BioGRID-ORCS; 32851; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32851; -.
DR PRO; PR:Q9VWS2; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030943; Expressed in egg cell and 28 other tissues.
DR GO; GO:0031965; C:nuclear membrane; IEA:InterPro.
DR GO; GO:0044613; C:nuclear pore central transport channel; ISS:FlyBase.
DR GO; GO:0044615; C:nuclear pore nuclear basket; IBA:GO_Central.
DR GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0017056; F:structural constituent of nuclear pore; ISS:FlyBase.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006607; P:NLS-bearing protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006999; P:nuclear pore organization; ISS:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR017389; Nucleoporin_NUP53.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR007846; RRM_NUP35_dom.
DR PANTHER; PTHR21527; PTHR21527; 1.
DR Pfam; PF05172; Nup35_RRM; 1.
DR PIRSF; PIRSF038119; Nucleoporin_NUP53; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS51472; RRM_NUP35; 1.
PE 1: Evidence at protein level;
KW mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Translocation; Transport.
FT CHAIN 1..331
FT /note="Nucleoporin Nup35"
FT /id="PRO_0000443735"
FT DOMAIN 187..268
FT /note="RRM Nup35-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00804"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 79..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 33..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 331 AA; 35099 MW; 455B6F2158727B26 CRC64;
MEPMNLGSPV NSPGSNQTQY LPPFLLGDPQ GITPHKNTLS PKTGRSNISF ATSPGGSSPH
ELNRSALSTR TLFAAQGGAH TAVGANSSTT AHGQTHSHHQ TGPPTQGLFD SLREQSVTPK
KKNHLGMLQL QSPNQSYQSS YHTNDSFAPG APNAINASMR ALCSPLGATA SPAGGLGTSV
TTGGNSRLSD FWVTIFGFPP GAGSMVLQHF TVCGTIVDVV HAPQNGNWMY VRYSSRIESD
KALNYNEKII AGNVMVGVSR CTDRSVIDKE NIGSVPNAEI GDPPTSPSAI RPFSQQSYKL
ARKDNIISPQ KDVPQKSSGL MDKAMDLIFG W