NUP42_MOUSE
ID NUP42_MOUSE Reviewed; 420 AA.
AC Q8CIC2; Q8BJX3; Q8BVP7; Q924S0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Nucleoporin NUP42 {ECO:0000305};
DE AltName: Full=NLP-1;
DE AltName: Full=Nucleoporin-like protein 2;
GN Name=Nup42; Synonyms=Nupl2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC STRAIN=BALB/cJ;
RA Tokuchi H., Fujita J.;
RL Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for the export of mRNAs containing poly(A) tails
CC from the nucleus into the cytoplasm. {ECO:0000250|UniProtKB:O15504}.
CC -!- SUBUNIT: Probable component of the nuclear pore complex (NPC).
CC Interacts with nuclear export protein NXF1. Interacts with GLE1. Able
CC to form a heterotrimer with NUP155 and GLE1 in vitro (By similarity).
CC Interacts with XPO1 (By similarity). {ECO:0000250|UniProtKB:O15504}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:O15504}. Nucleus membrane
CC {ECO:0000250|UniProtKB:O15504}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:O15504}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:O15504}. Note=Excluded from the nucleolus.
CC {ECO:0000250|UniProtKB:O15504}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CIC2-1; Sequence=Displayed;
CC Name=2; Synonyms=T;
CC IsoId=Q8CIC2-2; Sequence=VSP_016483, VSP_016484;
CC -!- DOMAIN: The FG repeats are interaction sites for karyopherins
CC (importins, exportins) and form probably an affinity gradient, guiding
CC the transport proteins unidirectionally with their cargo through the
CC NPC. {ECO:0000250|UniProtKB:O15504}.
CC -!- PTM: O-glycosylated. {ECO:0000250|UniProtKB:O15504}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC36589.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB067574; BAB62307.1; -; mRNA.
DR EMBL; AK077066; BAC36589.1; ALT_FRAME; mRNA.
DR EMBL; AK078478; BAC37297.1; -; mRNA.
DR EMBL; BC033270; AAH33270.1; -; mRNA.
DR CCDS; CCDS19115.1; -. [Q8CIC2-1]
DR RefSeq; NP_001333511.1; NM_001346582.1.
DR RefSeq; NP_694732.3; NM_153092.4.
DR AlphaFoldDB; Q8CIC2; -.
DR SMR; Q8CIC2; -.
DR BioGRID; 231073; 5.
DR ComplexPortal; CPX-4474; Nuclear pore complex.
DR STRING; 10090.ENSMUSP00000062766; -.
DR iPTMnet; Q8CIC2; -.
DR PhosphoSitePlus; Q8CIC2; -.
DR EPD; Q8CIC2; -.
DR MaxQB; Q8CIC2; -.
DR PaxDb; Q8CIC2; -.
DR PeptideAtlas; Q8CIC2; -.
DR PRIDE; Q8CIC2; -.
DR ProteomicsDB; 294255; -. [Q8CIC2-1]
DR ProteomicsDB; 294256; -. [Q8CIC2-2]
DR GeneID; 231042; -.
DR KEGG; mmu:231042; -.
DR CTD; 231042; -.
DR MGI; MGI:2387631; Nupl2.
DR eggNOG; ENOG502R2TD; Eukaryota.
DR InParanoid; Q8CIC2; -.
DR OrthoDB; 1562631at2759; -.
DR PhylomeDB; Q8CIC2; -.
DR Reactome; R-MMU-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-MMU-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-MMU-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-MMU-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-MMU-170822; Regulation of Glucokinase by Glucokinase Regulatory Protein.
DR Reactome; R-MMU-191859; snRNP Assembly.
DR Reactome; R-MMU-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-MMU-3232142; SUMOylation of ubiquitinylation proteins.
DR Reactome; R-MMU-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-MMU-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-MMU-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-MMU-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-MMU-4570464; SUMOylation of RNA binding proteins.
DR Reactome; R-MMU-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-MMU-5578749; Transcriptional regulation by small RNAs.
DR BioGRID-ORCS; 231042; 6 hits in 76 CRISPR screens.
DR ChiTaRS; Nupl2; mouse.
DR PRO; PR:Q8CIC2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8CIC2; protein.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005635; C:nuclear envelope; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IC:ComplexPortal.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0001750; C:photoreceptor outer segment; IDA:MGI.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005049; F:nuclear export signal receptor activity; ISO:MGI.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0006913; P:nucleocytoplasmic transport; IC:ComplexPortal.
DR GO; GO:0006611; P:protein export from nucleus; ISO:MGI.
DR InterPro; IPR000571; Znf_CCCH.
DR SMART; SM00356; ZnF_C3H1; 1.
DR PROSITE; PS50103; ZF_C3H1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Metal-binding;
KW mRNA transport; Nuclear pore complex; Nucleus; Protein transport;
KW Reference proteome; Repeat; Translocation; Transport; Zinc; Zinc-finger.
FT CHAIN 1..420
FT /note="Nucleoporin NUP42"
FT /id="PRO_0000204896"
FT REPEAT 14..15
FT /note="FG 1"
FT REPEAT 82..83
FT /note="FG 2"
FT REPEAT 95..96
FT /note="FG 3"
FT REPEAT 218..219
FT /note="FG 4"
FT REPEAT 220..221
FT /note="FG 5"
FT REPEAT 228..229
FT /note="FG 6"
FT REPEAT 265..266
FT /note="FG 7"
FT REPEAT 271..272
FT /note="FG 8"
FT REPEAT 288..289
FT /note="FG 9"
FT REPEAT 345..346
FT /note="FG 10"
FT REPEAT 364..365
FT /note="FG 11"
FT ZN_FING 1..25
FT /note="C3H1-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT REGION 25..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 365..420
FT /note="Interaction with GLE1"
FT /evidence="ECO:0000250"
FT COMPBIAS 42..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 84..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 149..160
FT /note="GFTDISPEELRL -> AKLCSTACKSVE (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_016483"
FT VAR_SEQ 161..420
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_016484"
FT CONFLICT 137
FT /note="V -> A (in Ref. 2; BAC36589)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="R -> G (in Ref. 2; BAC36589)"
FT /evidence="ECO:0000305"
FT CONFLICT 308
FT /note="A -> G (in Ref. 2; BAC36589/BAC37297)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="V -> A (in Ref. 2; BAC36589/BAC37297)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="A -> T (in Ref. 2; BAC37297)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 420 AA; 44337 MW; FE2CDDBD6F17492A CRC64;
MTICQFFLQG RCRFGDRCWN EHPGARGAGG ARQPPPQQQP PSGNNRRGWN ASSQRYSNVI
QPSSFPKSTP WGGSRDQDKP PFGSFDSGAS TSRGFGSSQN PFASPLSDEQ KDEKKLLEGI
VKDVEVWESS GQWMFSVYSP VRKKPNISGF TDISPEELRL EYHNFLTSNN LQSYLNSVQQ
LVSQWRNRIN ELKNLTMSTK GALLSDVKDG VSQAVPAFGF GSKQAGSFGS PGFPVNNSSS
STVQNFSFKT SPGLATPPSG STSVFGSHPA FGAGPSAGSS ISSSTPAFGL GKPEATSAAS
FSFKSPEASS FASPGFSGFP ASMAASPSGS TTAPPLRSGS SVVGFGSPSP HSQAVFAKPS
TDVFGGSGIS TSVLASGAAD NALFTPRDQL MKEELEQFQS QRFTLGKIPL KPPPVELLTV
