ID   NUP49_CHATD             Reviewed;         470 AA.
AC   G0S4X2; G3EQ72;
DT   24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Nucleoporin NUP49 {ECO:0000303|PubMed:21784248};
DE   AltName: Full=Nuclear pore protein NUP49;
GN   Name=NUP49; ORFNames=CTHT_0031980;
OS   Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Chaetomiaceae; Chaetomium.
OX   NCBI_TaxID=759272;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 1495 / CBS 144.50 / IMI 039719;
RX   PubMed=21784248; DOI=10.1016/j.cell.2011.06.039;
RA   Amlacher S., Sarges P., Flemming D., van Noort V., Kunze R., Devos D.P.,
RA   Arumugam M., Bork P., Hurt E.;
RT   "Insight into structure and assembly of the nuclear pore complex by
RT   utilizing the genome of a eukaryotic thermophile.";
RL   Cell 146:277-289(2011).
CC   -!- FUNCTION: Functions as a component of the nuclear pore complex (NPC).
CC       NPC components, collectively referred to as nucleoporins (NUPs), can
CC       play the role of both NPC structural components and of docking or
CC       interaction partners for transiently associated nuclear transport
CC       factors. Active directional transport is assured by both, a Phe-Gly
CC       (FG) repeat affinity gradient for these transport factors across the
CC       NPC and a transport cofactor concentration gradient across the nuclear
CC       envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in
CC       the nucleus, with GDP in the cytoplasm). NUP49 plays an important role
CC       in several nuclear transport pathways including poly(A)+ RNA, tRNA, and
CC       pre-ribosome transport. {ECO:0000250|UniProtKB:Q02199}.
CC   -!- SUBUNIT: Component of the nuclear pore complex (NPC). NPC constitutes
CC       the exclusive means of nucleocytoplasmic transport. NPCs allow the
CC       passive diffusion of ions and small molecules and the active, nuclear
CC       transport receptor-mediated bidirectional transport of macromolecules
CC       such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal
CC       subunits across the nuclear envelope. Due to its 8-fold rotational
CC       symmetry, all subunits are present with 8 copies or multiples thereof.
CC       {ECO:0000250|UniProtKB:Q02199, ECO:0000305|PubMed:21784248}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nuclear pore complex
CC       {ECO:0000250|UniProtKB:Q02199}. Nucleus membrane
CC       {ECO:0000250|UniProtKB:Q02199}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q02199}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q02199}. Nucleus membrane
CC       {ECO:0000250|UniProtKB:Q02199}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q02199}; Nucleoplasmic side
CC       {ECO:0000250|UniProtKB:Q02199}. Note=Symmetric distribution.
CC       {ECO:0000250|UniProtKB:Q02199}.
CC   -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC       karyopherins (importins, exportins) and form probably an affinity
CC       gradient, guiding the transport proteins unidirectionally with their
CC       cargo through the NPC. FG repeat regions are highly flexible and lack
CC       ordered secondary structure. The overall conservation of FG repeats
CC       regarding exact sequence, spacing, and repeat unit length is limited.
CC       FG repeat types and their physico-chemical environment change across
CC       the NPC from the nucleoplasmic to the cytoplasmic side: GLFG repeats
CC       are especially abundant in NUPs in the central region (lacking a
CC       charged environment but are enriched in Ser, Thr, Gln, and Asn).
CC       {ECO:0000250|UniProtKB:Q02199}.
CC   -!- SIMILARITY: Belongs to the nucleoporin GLFG family. {ECO:0000305}.
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DR   EMBL; GL988041; EGS21343.1; -; Genomic_DNA.
DR   EMBL; JF276282; AEN86175.1; -; Genomic_DNA.
DR   RefSeq; XP_006693639.1; XM_006693576.1.
DR   PDB; 5CWS; X-ray; 3.77 A; D/J=246-470.
DR   PDBsum; 5CWS; -.
DR   AlphaFoldDB; G0S4X2; -.
DR   SMR; G0S4X2; -.
DR   DIP; DIP-61841N; -.
DR   IntAct; G0S4X2; 5.
DR   STRING; 759272.G0S4X2; -.
DR   TCDB; 1.I.1.1.2; the nuclear pore complex (npc) family.
DR   ABCD; G0S4X2; 1 sequenced antibody.
DR   EnsemblFungi; EGS21343; EGS21343; CTHT_0031980.
DR   GeneID; 18257236; -.
DR   KEGG; cthr:CTHT_0031980; -.
DR   eggNOG; KOG0845; Eukaryota.
DR   HOGENOM; CLU_027081_1_0_1; -.
DR   OrthoDB; 1043980at2759; -.
DR   Proteomes; UP000008066; Unassembled WGS sequence.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR   GO; GO:0008139; F:nuclear localization sequence binding; IEA:InterPro.
DR   GO; GO:0017056; F:structural constituent of nuclear pore; IEA:InterPro.
DR   GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR025574; Nucleoporin_FG_rpt.
DR   InterPro; IPR024882; Nucleoporin_p58/p45.
DR   PANTHER; PTHR13437; PTHR13437; 1.
DR   Pfam; PF13634; Nucleoporin_FG; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Coiled coil; Membrane; mRNA transport; Nuclear pore complex;
KW   Nucleus; Protein transport; Reference proteome; Repeat; Translocation;
KW   Transport.
FT   CHAIN           1..470
FT                   /note="Nucleoporin NUP49"
FT                   /id="PRO_0000433176"
FT   REPEAT          17..20
FT                   /note="GLFG 1"
FT   REPEAT          45..48
FT                   /note="GLFG 2"
FT   REPEAT          63..66
FT                   /note="GLFG 3"
FT   REPEAT          79..82
FT                   /note="GLFG 4"
FT   REPEAT          96..99
FT                   /note="GLFG 5"
FT   REPEAT          111..114
FT                   /note="GLFG 6"
FT   REPEAT          127..130
FT                   /note="GLFG 7"
FT   REPEAT          142..145
FT                   /note="GLFG 8"
FT   REPEAT          156..159
FT                   /note="GLFG 9"
FT   REPEAT          168..171
FT                   /note="GLFG 10"
FT   REPEAT          181..184
FT                   /note="GLFG 11"
FT   REPEAT          193..197
FT                   /note="GLFG 12"
FT   REGION          1..92
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          196..221
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          239..270
FT                   /evidence="ECO:0000255"
FT   COILED          375..401
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   470 AA;  48589 MW;  5904EB28F07DBA16 CRC64;
     MSLFGTNTTS QTPAGGGLFG TTTSQSAQTG SLFGTATSQP QQTGGLFGST ATQTPSSQLQ
     STGLFGSTTA TSQPQQTGGL FGSTTTTTSQ PQQTGGLFGA TATSQPQSTG GLFGNTTTTS
     QPAQTVGLFG TTTQPQPAQS GGLFGSATQQ KPATGGLFGS TTTNTGAGLF GNTSNTIGGG
     GLFGQTAKPA TGGLFGQSTT QPQQQQNATP GLTMGQSTNT QQQVVPGVRI DLSNIKSTTR
     FNDLTEALQQ EIAKIDEEIQ KCIRDKEAVD AFLPAHGEQL AAIPTDVNFV TRKSEGAHNA
     LSSDILAIDQ LRELVKQDAD NARLSFKAID NLKLPMQYHQ AGLWSKQMGG AGTAGASGAS
     ADADGQSNAD LISYFSKTAD EMEEMMKKFE KTITEIEAHL TGVEAHAMAM QNVAAQSRNA
     AQGGVDERVY ELAAVLREFE ESILKVAGVV GGVKEGVTEL QLRDFMGHGS