NUP50_DROME
ID NUP50_DROME Reviewed; 564 AA.
AC Q7K0D8;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Nuclear pore complex protein Nup50 {ECO:0000305};
DE AltName: Full=50 kDa nucleoporin {ECO:0000312|FlyBase:FBgn0033264};
GN Name=Nup50 {ECO:0000312|FlyBase:FBgn0033264};
GN ORFNames=CG2158 {ECO:0000303|PubMed:20144760,
GN ECO:0000312|FlyBase:FBgn0033264};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL48017.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL48017.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAL48017.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=20144760; DOI=10.1016/j.cell.2010.01.011;
RA Kalverda B., Pickersgill H., Shloma V.V., Fornerod M.;
RT "Nucleoporins directly stimulate expression of developmental and cell-cycle
RT genes inside the nucleoplasm.";
RL Cell 140:360-371(2010).
CC -!- FUNCTION: Component of the nuclear pore complex that has a direct role
CC in nuclear protein import (By similarity). Actively displaces NLSs from
CC importin-alpha, and facilitates disassembly of the importin-alpha:beta-
CC cargo complex and importin recycling (By similarity). Binds to
CC transcriptionally active chromatin sites when located in the
CC nucleoplasm and is involved in transcriptional activation
CC (PubMed:20144760). {ECO:0000250|UniProtKB:Q9JIH2,
CC ECO:0000250|UniProtKB:Q9UKX7, ECO:0000269|PubMed:20144760}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:20144760}. Nucleus envelope
CC {ECO:0000269|PubMed:20144760}. Chromosome
CC {ECO:0000269|PubMed:20144760}. Nucleus, nuclear pore complex
CC {ECO:0000250|UniProtKB:O08587}. Nucleus membrane; Peripheral membrane
CC protein; Nucleoplasmic side {ECO:0000250|UniProtKB:O08587}.
CC Note=Associates with chromatin (PubMed:20144760).
CC -!- DOMAIN: Contains FG repeats. FG repeats are interaction sites for
CC karyopherins (importins, exportins) and form probably an affinity
CC gradient, guiding the transport proteins unidirectionally with their
CC cargo through the NPC. FG repeat regions are highly flexible and lack
CC ordered secondary structure. The overall conservation of FG repeats
CC regarding exact sequence, spacing, and repeat unit length is limited.
CC {ECO:0000305}.
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DR EMBL; AE013599; AAF59131.1; -; Genomic_DNA.
DR EMBL; AY070546; AAL48017.1; -; mRNA.
DR RefSeq; NP_610355.1; NM_136511.3.
DR AlphaFoldDB; Q7K0D8; -.
DR SMR; Q7K0D8; -.
DR DIP; DIP-21255N; -.
DR IntAct; Q7K0D8; 5.
DR MINT; Q7K0D8; -.
DR STRING; 7227.FBpp0087861; -.
DR PaxDb; Q7K0D8; -.
DR PRIDE; Q7K0D8; -.
DR DNASU; 35784; -.
DR EnsemblMetazoa; FBtr0088783; FBpp0087861; FBgn0033264.
DR GeneID; 35784; -.
DR KEGG; dme:Dmel_CG2158; -.
DR UCSC; CG2158-RA; d. melanogaster.
DR CTD; 10762; -.
DR FlyBase; FBgn0033264; Nup50.
DR VEuPathDB; VectorBase:FBgn0033264; -.
DR eggNOG; KOG2724; Eukaryota.
DR GeneTree; ENSGT00440000035348; -.
DR HOGENOM; CLU_032593_0_0_1; -.
DR InParanoid; Q7K0D8; -.
DR OMA; GIPCQRM; -.
DR OrthoDB; 793797at2759; -.
DR PhylomeDB; Q7K0D8; -.
DR Reactome; R-DME-159227; Transport of the SLBP independent Mature mRNA.
DR Reactome; R-DME-159230; Transport of the SLBP Dependant Mature mRNA.
DR Reactome; R-DME-159231; Transport of Mature mRNA Derived from an Intronless Transcript.
DR Reactome; R-DME-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DME-3108214; SUMOylation of DNA damage response and repair proteins.
DR Reactome; R-DME-3301854; Nuclear Pore Complex (NPC) Disassembly.
DR Reactome; R-DME-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-DME-4085377; SUMOylation of SUMOylation proteins.
DR Reactome; R-DME-4551638; SUMOylation of chromatin organization proteins.
DR Reactome; R-DME-4615885; SUMOylation of DNA replication proteins.
DR Reactome; R-DME-5578749; Transcriptional regulation by small RNAs.
DR SignaLink; Q7K0D8; -.
DR BioGRID-ORCS; 35784; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35784; -.
DR PRO; PR:Q7K0D8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033264; Expressed in eye disc (Drosophila) and 50 other tissues.
DR GO; GO:0000785; C:chromatin; IMP:UniProtKB.
DR GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005643; C:nuclear pore; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005704; C:polytene chromosome band; IDA:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; IMP:UniProtKB.
DR GO; GO:0035080; P:heat shock-mediated polytene chromosome puffing; IDA:UniProtKB.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006606; P:protein import into nucleus; IMP:FlyBase.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR015007; NUP2/50/61.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR000156; Ran_bind_dom.
DR InterPro; IPR045255; RanBP1-like.
DR PANTHER; PTHR23138; PTHR23138; 2.
DR Pfam; PF08911; NUP50; 1.
DR Pfam; PF00638; Ran_BP1; 1.
DR SMART; SM00160; RanBD; 1.
PE 2: Evidence at transcript level;
KW Activator; Chromosome; Membrane; mRNA transport; Nuclear pore complex;
KW Nucleus; Protein transport; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Translocation; Transport.
FT CHAIN 1..564
FT /note="Nuclear pore complex protein Nup50"
FT /id="PRO_0000439235"
FT REPEAT 77..78
FT /note="1"
FT /evidence="ECO:0000305"
FT REPEAT 114..115
FT /note="2"
FT /evidence="ECO:0000305"
FT REPEAT 164..165
FT /note="3"
FT /evidence="ECO:0000305"
FT REPEAT 278..279
FT /note="4"
FT /evidence="ECO:0000305"
FT REPEAT 316..317
FT /note="5"
FT /evidence="ECO:0000305"
FT REPEAT 356..357
FT /note="6"
FT /evidence="ECO:0000305"
FT REPEAT 380..381
FT /note="7"
FT /evidence="ECO:0000305"
FT REPEAT 398..399
FT /note="8"
FT /evidence="ECO:0000305"
FT DOMAIN 455..564
FT /note="RanBD1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00164"
FT REPEAT 474..475
FT /note="9"
FT /evidence="ECO:0000305"
FT REGION 1..197
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..475
FT /note="9 X 2 AA repeats of F-G"
FT /evidence="ECO:0000305"
FT REGION 237..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 339..383
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 425..447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..65
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..196
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 246..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 368..383
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 564 AA; 59434 MW; DC81FD1663EF89F5 CRC64;
MAGKRQATSN LNHENWDLEE EPEERGTFRT ATEEELKTRV IKKARRKIAG GSSAAEEDGA
EEKTAEPKSV FSGFSGFGKP AASPAAGSPF SFLANVTAPA TTSSSEPKKS AFSFGFSSSS
SSADRPVSTS ICGTASTSST APSPLPAKES TSTVDGAKPT TSIFGNISAA KKESSEAKTS
SSSTSLTSTM ETSEYRESVA DLNRSVIKFL QDQMGKSPYC ILTPVFKNYD EHLKDLQDEE
SARTNSTKSK TAQARSQEPV AKVSRASSPP KAATTFTFGK PSAPIGASVS PLAKKPNCTI
TSGGTTTTTA TPLVSFGSTA SFTAPVPSSS SIFSLTAKPT GEAKSDDTPK SSIFSFGAKD
TTTKKDEPNF SAPKTNGFSF GLKSNNDDKP STSLFAGFGK APGGAGDGAK GFSFTNSATP
FSLGNIHPPA AAAAPAEEEK EEDTPPKVEF KQVVEDDAIY SKRCKVFIKK DKDFGDRGVG
TLYLKPVKDS EKIQLLVRAD TNLGNILVNL ILSKGIPCQR MGKNNVLMVC VPTPEDSKAT
SLLLRVKTGD EADDLLEKIK EHIK
